CX6A1_RAT
ID CX6A1_RAT Reviewed; 111 AA.
AC P10818;
DT 01-JUL-1989, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1996, sequence version 2.
DT 03-AUG-2022, entry version 154.
DE RecName: Full=Cytochrome c oxidase subunit 6A1, mitochondrial;
DE AltName: Full=Cytochrome c oxidase polypeptide VIa-liver;
DE Flags: Precursor;
GN Name=Cox6a1; Synonyms=Cox6al;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=Fischer; TISSUE=Liver;
RX PubMed=8144025; DOI=10.1016/0378-1119(94)90542-8;
RA Mell O.C., Seibel P., Kadenbach B.;
RT "Structural organisation of the rat genes encoding liver- and heart-type of
RT cytochrome c oxidase subunit VIa and a pseudogene related to the COXVIa-L
RT cDNA.";
RL Gene 140:179-186(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE OF 27-111.
RC TISSUE=Liver;
RX PubMed=2461293; DOI=10.1002/j.1460-2075.1988.tb03083.x;
RA Schlerf A., Droste M., Winter M., Kadenbach B.;
RT "Characterization of two different genes (cDNA) for cytochrome c oxidase
RT subunit VIa from heart and liver of the rat.";
RL EMBO J. 7:2387-2391(1988).
RN [3]
RP PROTEIN SEQUENCE OF 27-48.
RC TISSUE=Brown adipose tissue, and Liver;
RX PubMed=2153407; DOI=10.1016/0005-2728(90)90042-3;
RA Kadenbach B., Stroh A., Becker A., Eckersorn C., Lottspeich F.;
RT "Tissue- and species-specific expression of cytochrome c oxidase isozymes
RT in vertebrates.";
RL Biochim. Biophys. Acta 1015:368-372(1990).
RN [4]
RP PROTEIN SEQUENCE OF 27-49.
RC STRAIN=Wistar; TISSUE=Liver;
RX PubMed=7601105; DOI=10.1111/j.1432-1033.1995.tb20556.x;
RA Schaegger H., Noack H., Halangk W., Brandt U., von Jagow G.;
RT "Cytochrome-c oxidase in developing rat heart. Enzymic properties and
RT amino-terminal sequences suggest identity of the fetal heart and the adult
RT liver isoform.";
RL Eur. J. Biochem. 230:235-241(1995).
RN [5]
RP PROTEIN SEQUENCE OF 65-80, AND IDENTIFICATION BY MASS SPECTROMETRY.
RC STRAIN=Sprague-Dawley; TISSUE=Hippocampus;
RA Lubec G., Diao W.;
RL Submitted (APR-2007) to UniProtKB.
CC -!- FUNCTION: Component of the cytochrome c oxidase, the last enzyme in the
CC mitochondrial electron transport chain which drives oxidative
CC phosphorylation. The respiratory chain contains 3 multisubunit
CC complexes succinate dehydrogenase (complex II, CII), ubiquinol-
CC cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III,
CC CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to
CC transfer electrons derived from NADH and succinate to molecular oxygen,
CC creating an electrochemical gradient over the inner membrane that
CC drives transmembrane transport and the ATP synthase. Cytochrome c
CC oxidase is the component of the respiratory chain that catalyzes the
CC reduction of oxygen to water. Electrons originating from reduced
CC cytochrome c in the intermembrane space (IMS) are transferred via the
CC dinuclear copper A center (CU(A)) of subunit 2 and heme A of subunit 1
CC to the active site in subunit 1, a binuclear center (BNC) formed by
CC heme A3 and copper B (CU(B)). The BNC reduces molecular oxygen to 2
CC water molecules unsing 4 electrons from cytochrome c in the IMS and 4
CC protons from the mitochondrial matrix. {ECO:0000250|UniProtKB:P32799}.
CC -!- PATHWAY: Energy metabolism; oxidative phosphorylation.
CC {ECO:0000250|UniProtKB:P32799}.
CC -!- SUBUNIT: Component of the cytochrome c oxidase (complex IV, CIV), a
CC multisubunit enzyme composed of 14 subunits. The complex is composed of
CC a catalytic core of 3 subunits MT-CO1, MT-CO2 and MT-CO3, encoded in
CC the mitochondrial DNA, and 11 supernumerary subunits COX4I, COX5A,
CC COX5B, COX6A, COX6B, COX6C, COX7A, COX7B, COX7C, COX8 and NDUFA4, which
CC are encoded in the nuclear genome. The complex exists as a monomer or a
CC dimer and forms supercomplexes (SCs) in the inner mitochondrial
CC membrane with NADH-ubiquinone oxidoreductase (complex I, CI) and
CC ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex
CC III, CIII), resulting in different assemblies (supercomplex
CC SCI(1)III(2)IV(1) and megacomplex MCI(2)III(2)IV(2)).
CC {ECO:0000250|UniProtKB:P12074}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane
CC {ECO:0000250|UniProtKB:P12074}; Single-pass membrane protein
CC {ECO:0000250|UniProtKB:P12074}.
CC -!- SIMILARITY: Belongs to the cytochrome c oxidase subunit 6A family.
CC {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; X72757; CAA51286.1; ALT_SEQ; Genomic_DNA.
DR EMBL; X12553; CAA31067.1; -; mRNA.
DR PIR; S01156; S01156.
DR PIR; S65379; S65379.
DR RefSeq; NP_036946.1; NM_012814.1.
DR AlphaFoldDB; P10818; -.
DR SMR; P10818; -.
DR CORUM; P10818; -.
DR STRING; 10116.ENSRNOP00000001545; -.
DR jPOST; P10818; -.
DR PaxDb; P10818; -.
DR PRIDE; P10818; -.
DR DNASU; 25282; -.
DR GeneID; 25282; -.
DR KEGG; rno:25282; -.
DR UCSC; RGD:2384; rat.
DR CTD; 1337; -.
DR RGD; 2384; Cox6a1.
DR VEuPathDB; HostDB:ENSRNOG00000001170; -.
DR eggNOG; KOG3469; Eukaryota.
DR HOGENOM; CLU_122515_1_1_1; -.
DR InParanoid; P10818; -.
DR OMA; GYEGHDE; -.
DR OrthoDB; 1591077at2759; -.
DR PhylomeDB; P10818; -.
DR TreeFam; TF105064; -.
DR Reactome; R-RNO-5628897; TP53 Regulates Metabolic Genes.
DR Reactome; R-RNO-611105; Respiratory electron transport.
DR Reactome; R-RNO-9707564; Cytoprotection by HMOX1.
DR UniPathway; UPA00705; -.
DR PRO; PR:P10818; -.
DR Proteomes; UP000002494; Chromosome 12.
DR Bgee; ENSRNOG00000001170; Expressed in cerebellum and 20 other tissues.
DR Genevisible; P10818; RN.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0031966; C:mitochondrial membrane; ISO:RGD.
DR GO; GO:0005751; C:mitochondrial respiratory chain complex IV; ISO:RGD.
DR GO; GO:0004129; F:cytochrome-c oxidase activity; IEA:InterPro.
DR GO; GO:0030234; F:enzyme regulator activity; IBA:GO_Central.
DR GO; GO:0006123; P:mitochondrial electron transport, cytochrome c to oxygen; IBA:GO_Central.
DR CDD; cd00925; Cyt_c_Oxidase_VIa; 1.
DR Gene3D; 4.10.95.10; -; 1.
DR InterPro; IPR001349; Cyt_c_oxidase_su6a.
DR InterPro; IPR018507; Cyt_c_oxidase_su6a_CS.
DR InterPro; IPR036418; Cyt_c_oxidase_su6a_sf.
DR PANTHER; PTHR11504; PTHR11504; 1.
DR Pfam; PF02046; COX6A; 1.
DR PIRSF; PIRSF000277; COX6A1; 1.
DR SUPFAM; SSF81411; SSF81411; 1.
DR PROSITE; PS01329; COX6A; 1.
PE 1: Evidence at protein level;
KW Direct protein sequencing; Membrane; Mitochondrion;
KW Mitochondrion inner membrane; Oxidoreductase; Reference proteome;
KW Transit peptide; Transmembrane; Transmembrane helix.
FT TRANSIT 1..26
FT /note="Mitochondrion"
FT /evidence="ECO:0000269|PubMed:2153407,
FT ECO:0000269|PubMed:7601105"
FT CHAIN 27..111
FT /note="Cytochrome c oxidase subunit 6A1, mitochondrial"
FT /id="PRO_0000006122"
FT TOPO_DOM 27..36
FT /note="Mitochondrial matrix"
FT /evidence="ECO:0000250|UniProtKB:P07471"
FT TRANSMEM 37..61
FT /note="Helical"
FT /evidence="ECO:0000250|UniProtKB:P07471"
FT TOPO_DOM 62..111
FT /note="Mitochondrial intermembrane"
FT /evidence="ECO:0000250|UniProtKB:P07471"
SQ SEQUENCE 111 AA; 12301 MW; 71EA06E23ED7546D CRC64;
MASAVLSASR VSGLLGRALP RVGRPMSSGA HGEEGSARIW KALTYFVALP GVGVSMLNVF
LKSRHEEHER PEFVAYPHLR IRTKPFPWGD GNHTLFHNPH MNPLPTGYED E
