CX6B1_ARATH
ID CX6B1_ARATH Reviewed; 191 AA.
AC Q9S7L9; Q8LCP1;
DT 21-SEP-2011, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 25-MAY-2022, entry version 127.
DE RecName: Full=Cytochrome c oxidase subunit 6b-1;
DE Short=AtCOX6b-1;
GN Name=COX6B-1; OrderedLocusNames=At1g22450; ORFNames=F12K8.20;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], GENE FAMILY, NOMENCLATURE, AND TISSUE
RP SPECIFICITY.
RX PubMed=11250078; DOI=10.1016/s0378-1119(01)00334-1;
RA Ohtsu K., Nakazono M., Tsutsumi N., Hirai A.;
RT "Characterization and expression of the genes for cytochrome c oxidase
RT subunit VIb (COX6b) from rice and Arabidopsis thaliana.";
RL Gene 264:233-239(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP DEVELOPMENTAL STAGE.
RX PubMed=11434463; DOI=10.1266/ggs.76.89;
RA Saisho D., Nakazono M., Lee K.-H., Tsutsumi N., Akita S., Hirai A.;
RT "The gene for alternative oxidase-2 (AOX2) from Arabidopsis thaliana
RT consists of five exons unlike other AOX genes and is transcribed at an
RT early stage during germination.";
RL Genes Genet. Syst. 76:89-97(2001).
RN [7]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, CLEAVAGE OF INITIATOR
RP METHIONINE [LARGE SCALE ANALYSIS], AND IDENTIFICATION BY MASS SPECTROMETRY
RP [LARGE SCALE ANALYSIS].
RX PubMed=22223895; DOI=10.1074/mcp.m111.015131;
RA Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T.,
RA Giglione C.;
RT "Comparative large-scale characterisation of plant vs. mammal proteins
RT reveals similar and idiosyncratic N-alpha acetylation features.";
RL Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012).
CC -!- FUNCTION: This protein is one of the nuclear-coded polypeptide chains
CC of cytochrome c oxidase, the terminal oxidase in mitochondrial electron
CC transport. This protein may be one of the heme-binding subunits of the
CC oxidase.
CC -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Expressed in the whole plant.
CC {ECO:0000269|PubMed:11250078}.
CC -!- DEVELOPMENTAL STAGE: Gradually expressed after germination.
CC {ECO:0000269|PubMed:11434463}.
CC -!- SIMILARITY: Belongs to the cytochrome c oxidase subunit 6B (TC 3.D.4.8)
CC family. {ECO:0000305}.
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DR EMBL; AB035444; BAA87883.1; -; mRNA.
DR EMBL; AC006551; AAF18532.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE30243.1; -; Genomic_DNA.
DR EMBL; AY063997; AAL36353.1; -; mRNA.
DR EMBL; AY096696; AAM20330.1; -; mRNA.
DR EMBL; AY086483; AAM63485.1; -; mRNA.
DR PIR; F86357; F86357.
DR RefSeq; NP_173661.1; NM_102094.4.
DR AlphaFoldDB; Q9S7L9; -.
DR SMR; Q9S7L9; -.
DR BioGRID; 24090; 1.
DR IntAct; Q9S7L9; 1.
DR STRING; 3702.AT1G22450.1; -.
DR iPTMnet; Q9S7L9; -.
DR PaxDb; Q9S7L9; -.
DR PRIDE; Q9S7L9; -.
DR ProteomicsDB; 220429; -.
DR EnsemblPlants; AT1G22450.1; AT1G22450.1; AT1G22450.
DR GeneID; 838851; -.
DR Gramene; AT1G22450.1; AT1G22450.1; AT1G22450.
DR KEGG; ath:AT1G22450; -.
DR Araport; AT1G22450; -.
DR TAIR; locus:2009497; AT1G22450.
DR eggNOG; KOG3057; Eukaryota.
DR HOGENOM; CLU_084057_0_0_1; -.
DR InParanoid; Q9S7L9; -.
DR OMA; SWNEQRE; -.
DR OrthoDB; 1586678at2759; -.
DR PRO; PR:Q9S7L9; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9S7L9; baseline and differential.
DR Genevisible; Q9S7L9; AT.
DR GO; GO:0009535; C:chloroplast thylakoid membrane; HDA:TAIR.
DR GO; GO:0005739; C:mitochondrion; HDA:TAIR.
DR GO; GO:0005634; C:nucleus; HDA:TAIR.
DR GO; GO:0045277; C:respiratory chain complex IV; IEA:InterPro.
DR GO; GO:0009579; C:thylakoid; HDA:TAIR.
DR GO; GO:0005507; F:copper ion binding; HDA:TAIR.
DR CDD; cd00926; Cyt_c_Oxidase_VIb; 1.
DR Gene3D; 1.10.10.140; -; 1.
DR InterPro; IPR003213; Cyt_c_oxidase_su6B.
DR InterPro; IPR036549; Cyt_c_oxidase_su6B_sf.
DR Pfam; PF02297; COX6B; 1.
DR SUPFAM; SSF47694; SSF47694; 1.
DR PROSITE; PS51808; CHCH; 1.
PE 1: Evidence at protein level;
KW Acetylation; Disulfide bond; Mitochondrion; Reference proteome.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0007744|PubMed:22223895"
FT CHAIN 2..191
FT /note="Cytochrome c oxidase subunit 6b-1"
FT /id="PRO_0000412233"
FT DOMAIN 134..177
FT /note="CHCH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01150"
FT REGION 1..126
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 137..147
FT /note="Cx9C motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01150"
FT MOTIF 158..169
FT /note="Cx10C motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01150"
FT COMPBIAS 1..15
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 16..32
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 46..74
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 92..110
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 2
FT /note="N-acetylalanine"
FT /evidence="ECO:0007744|PubMed:22223895"
FT DISULFID 137..169
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01150"
FT DISULFID 147..158
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01150"
FT CONFLICT 87
FT /note="E -> Q (in Ref. 5; AAM63485)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 191 AA; 21195 MW; 7592D7DBC94BC054 CRC64;
MADAVNAQTP SLSEQYHLEK EVKQDTSAKP VEVKEVAPEV TTQAEEVKTE QAKEESPVEE
AVSVVEEKSE SAPESTEVAS EAPAAAEDNA EETPAAAEEN NDENASEEVA EETPDEIKLE
TAPADFRFPT TNQTRHCFTR YVEYHRCVAA KGDDAPECDK FAKFYRSLCP SEWVDRWNEQ
RENGTFPGPL P
