ID   34KD_MYCTU              Reviewed;         303 AA.
AC   P9WIR9; L0T875; P65637; P71556;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2014, sequence version 1.
DT   03-AUG-2022, entry version 33.
DE   RecName: Full=34 kDa antigenic protein homolog;
GN   OrderedLocusNames=Rv0954; ORFNames=MTCY10D7.20c;
OS   Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83332;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=9634230; DOI=10.1038/31159;
RA   Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA   Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA   Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA   Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA   Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA   Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA   Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA   Barrell B.G.;
RT   "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT   genome sequence.";
RL   Nature 393:537-544(1998).
RN   [2]
RP   ACETYLATION [LARGE SCALE ANALYSIS] AT THR-2, CLEAVAGE OF INITIATOR
RP   METHIONINE [LARGE SCALE ANALYSIS], AND IDENTIFICATION BY MASS SPECTROMETRY
RP   [LARGE SCALE ANALYSIS].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=21969609; DOI=10.1074/mcp.m111.011627;
RA   Kelkar D.S., Kumar D., Kumar P., Balakrishnan L., Muthusamy B., Yadav A.K.,
RA   Shrivastava P., Marimuthu A., Anand S., Sundaram H., Kingsbury R.,
RA   Harsha H.C., Nair B., Prasad T.S., Chauhan D.S., Katoch K., Katoch V.M.,
RA   Kumar P., Chaerkady R., Ramachandran S., Dash D., Pandey A.;
RT   "Proteogenomic analysis of Mycobacterium tuberculosis by high resolution
RT   mass spectrometry.";
RL   Mol. Cell. Proteomics 10:M111.011627-M111.011627(2011).
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000305}.
CC   -!- SIMILARITY: To M.paratuberculosis 34 kDa antigenic protein.
CC       {ECO:0000305}.
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DR   EMBL; AL123456; CCP43702.1; -; Genomic_DNA.
DR   PIR; H70716; H70716.
DR   RefSeq; NP_215469.1; NC_000962.3.
DR   RefSeq; WP_003404875.1; NZ_NVQJ01000001.1.
DR   AlphaFoldDB; P9WIR9; -.
DR   STRING; 83332.Rv0954; -.
DR   iPTMnet; P9WIR9; -.
DR   PaxDb; P9WIR9; -.
DR   DNASU; 885411; -.
DR   GeneID; 885411; -.
DR   KEGG; mtu:Rv0954; -.
DR   TubercuList; Rv0954; -.
DR   eggNOG; ENOG5033AAF; Bacteria.
DR   OMA; PRPKYDP; -.
DR   Proteomes; UP000001584; Chromosome.
DR   GO; GO:0005887; C:integral component of plasma membrane; HDA:MTBBASE.
DR   GO; GO:0009274; C:peptidoglycan-based cell wall; HDA:MTBBASE.
DR   InterPro; IPR035166; DUF5336.
DR   Pfam; PF17270; DUF5336; 1.
PE   1: Evidence at protein level;
KW   Acetylation; Cell membrane; Membrane; Reference proteome; Transmembrane;
KW   Transmembrane helix.
FT   INIT_MET        1
FT                   /note="Removed"
FT                   /evidence="ECO:0007744|PubMed:21969609"
FT   CHAIN           2..303
FT                   /note="34 kDa antigenic protein homolog"
FT                   /id="PRO_0000064364"
FT   TRANSMEM        42..62
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        77..97
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        102..122
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        134..154
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   REGION          194..303
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        213..259
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        271..297
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         2
FT                   /note="N-acetylthreonine"
FT                   /evidence="ECO:0007744|PubMed:21969609"
SQ   SEQUENCE   303 AA;  30204 MW;  4FE18A077FDFD8B5 CRC64;
     MTYSPGNPGY PQAQPAGSYG GVTPSFAHAD EGASKLPMYL NIAVAVLGLA AYFASFGPMF
     TLSTELGGGD GAVSGDTGLP VGVALLAALL AGVALVPKAK SHVTVVAVLG VLGVFLMVSA
     TFNKPSAYST GWALWVVLAF IVFQAVAAVL ALLVETGAIT APAPRPKFDP YGQYGRYGQY
     GQYGVQPGGY YGQQGAQQAA GLQSPGPQQS PQPPGYGSQY GGYSSSPSQS GSGYTAQPPA
     QPPAQSGSQQ SHQGPSTPPT GFPSFSPPPP VSAGTGSQAG SAPVNYSNPS GGEQSSSPGG
     APV