CX7A2_RAT
ID CX7A2_RAT Reviewed; 83 AA.
AC P35171;
DT 01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1994, sequence version 1.
DT 03-AUG-2022, entry version 148.
DE RecName: Full=Cytochrome c oxidase subunit 7A2, mitochondrial;
DE AltName: Full=Cytochrome c oxidase subunit VIIa-liver/heart;
DE Short=Cytochrome c oxidase subunit VIIa-L;
DE Flags: Precursor;
GN Name=Cox7a2; Synonyms=Cox7a3, Cox7al;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=Wistar; TISSUE=Liver;
RX PubMed=2175887; DOI=10.1093/nar/18.23.7143;
RA Enders C., Schlerf A., Mell O., Grossman L.I., Kadenbach B.;
RT "Nucleotide sequence of cDNA encoding subunit VIIa of rat liver cytochrome
RT c oxidase.";
RL Nucleic Acids Res. 18:7143-7143(1990).
RN [2]
RP PROTEIN SEQUENCE OF 24-47.
RC TISSUE=Heart;
RX PubMed=2175025; DOI=10.1203/00006450-199011000-00024;
RA Kennaway N.G., Carrero-Valenzuela R.D., Ewart G., Balan V.K.,
RA Lightowlers R.N., Zhang Y.-Z., Powell B.R., Capaldi R.A., Buist N.R.M.;
RT "Isoforms of mammalian cytochrome c oxidase: correlation with human
RT cytochrome c oxidase deficiency.";
RL Pediatr. Res. 28:529-535(1990).
RN [3]
RP PROTEIN SEQUENCE OF 24-33.
RC STRAIN=Wistar; TISSUE=Heart, and Liver;
RX PubMed=7601105; DOI=10.1111/j.1432-1033.1995.tb20556.x;
RA Schaegger H., Noack H., Halangk W., Brandt U., von Jagow G.;
RT "Cytochrome-c oxidase in developing rat heart. Enzymic properties and
RT amino-terminal sequences suggest identity of the fetal heart and the adult
RT liver isoform.";
RL Eur. J. Biochem. 230:235-241(1995).
RN [4]
RP PROTEIN SEQUENCE OF 47-56, AND IDENTIFICATION BY MASS SPECTROMETRY.
RC STRAIN=Sprague-Dawley; TISSUE=Brain;
RA Lubec G., Kang S.U.;
RL Submitted (JUL-2007) to UniProtKB.
CC -!- FUNCTION: Component of the cytochrome c oxidase, the last enzyme in the
CC mitochondrial electron transport chain which drives oxidative
CC phosphorylation. The respiratory chain contains 3 multisubunit
CC complexes succinate dehydrogenase (complex II, CII), ubiquinol-
CC cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III,
CC CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to
CC transfer electrons derived from NADH and succinate to molecular oxygen,
CC creating an electrochemical gradient over the inner membrane that
CC drives transmembrane transport and the ATP synthase. Cytochrome c
CC oxidase is the component of the respiratory chain that catalyzes the
CC reduction of oxygen to water. Electrons originating from reduced
CC cytochrome c in the intermembrane space (IMS) are transferred via the
CC dinuclear copper A center (CU(A)) of subunit 2 and heme A of subunit 1
CC to the active site in subunit 1, a binuclear center (BNC) formed by
CC heme A3 and copper B (CU(B)). The BNC reduces molecular oxygen to 2
CC water molecules using 4 electrons from cytochrome c in the IMS and 4
CC protons from the mitochondrial matrix. {ECO:0000250|UniProtKB:P10174}.
CC -!- PATHWAY: Energy metabolism; oxidative phosphorylation.
CC {ECO:0000250|UniProtKB:P10174}.
CC -!- SUBUNIT: Component of the cytochrome c oxidase (complex IV, CIV), a
CC multisubunit enzyme composed of 14 subunits. The complex is composed of
CC a catalytic core of 3 subunits MT-CO1, MT-CO2 and MT-CO3, encoded in
CC the mitochondrial DNA, and 11 supernumerary subunits COX4I, COX5A,
CC COX5B, COX6A, COX6B, COX6C, COX7A, COX7B, COX7C, COX8 and NDUFA4, which
CC are encoded in the nuclear genome. The complex exists as a monomer or a
CC dimer and forms supercomplexes (SCs) in the inner mitochondrial
CC membrane with NADH-ubiquinone oxidoreductase (complex I, CI) and
CC ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex
CC III, CIII), resulting in different assemblies (supercomplex
CC SCI(1)III(2)IV(1) and megacomplex MCI(2)III(2)IV(2)). Interacts with
CC PET100. {ECO:0000250|UniProtKB:P14406}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane
CC {ECO:0000250|UniProtKB:P14406}; Single-pass membrane protein
CC {ECO:0000250|UniProtKB:P14406}.
CC -!- SIMILARITY: Belongs to the cytochrome c oxidase VIIa family.
CC {ECO:0000305}.
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DR EMBL; X54080; CAA38017.1; -; mRNA.
DR PIR; S13099; S13099.
DR PIR; S65385; S65385.
DR RefSeq; NP_071948.1; NM_022503.2.
DR RefSeq; XP_003751415.1; XM_003751367.3.
DR RefSeq; XP_003752782.1; XM_003752734.3.
DR AlphaFoldDB; P35171; -.
DR SMR; P35171; -.
DR BioGRID; 248145; 1.
DR CORUM; P35171; -.
DR IntAct; P35171; 2.
DR MINT; P35171; -.
DR STRING; 10116.ENSRNOP00000037372; -.
DR CarbonylDB; P35171; -.
DR iPTMnet; P35171; -.
DR PhosphoSitePlus; P35171; -.
DR jPOST; P35171; -.
DR PaxDb; P35171; -.
DR PRIDE; P35171; -.
DR Ensembl; ENSRNOT00000038497; ENSRNOP00000094897; ENSRNOG00000027791.
DR GeneID; 29507; -.
DR GeneID; 688386; -.
DR KEGG; rno:29507; -.
DR KEGG; rno:688386; -.
DR UCSC; RGD:68365; rat.
DR CTD; 1347; -.
DR CTD; 688386; -.
DR RGD; 68365; Cox7a2.
DR VEuPathDB; HostDB:ENSRNOG00000042903; -.
DR eggNOG; ENOG502S4DT; Eukaryota.
DR GeneTree; ENSGT00940000154550; -.
DR HOGENOM; CLU_173437_0_0_1; -.
DR InParanoid; P35171; -.
DR OMA; MWRNVQA; -.
DR OrthoDB; 1548349at2759; -.
DR PhylomeDB; P35171; -.
DR TreeFam; TF105067; -.
DR UniPathway; UPA00705; -.
DR PRO; PR:P35171; -.
DR Proteomes; UP000002494; Chromosome 14.
DR Bgee; ENSRNOG00000042903; Expressed in heart and 19 other tissues.
DR ExpressionAtlas; P35171; baseline.
DR Genevisible; P35171; RN.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0031966; C:mitochondrial membrane; ISO:RGD.
DR GO; GO:0005746; C:mitochondrial respirasome; IBA:GO_Central.
DR GO; GO:0005751; C:mitochondrial respiratory chain complex IV; ISO:RGD.
DR GO; GO:0016491; F:oxidoreductase activity; IEA:UniProtKB-KW.
DR GO; GO:0006123; P:mitochondrial electron transport, cytochrome c to oxygen; IEA:InterPro.
DR GO; GO:0097250; P:mitochondrial respirasome assembly; IBA:GO_Central.
DR GO; GO:0002082; P:regulation of oxidative phosphorylation; IBA:GO_Central.
DR CDD; cd00928; Cyt_c_Oxidase_VIIa; 1.
DR Gene3D; 4.10.91.10; -; 1.
DR InterPro; IPR039297; COX7a.
DR InterPro; IPR036539; Cyt_c_oxidase_su7a_sf.
DR InterPro; IPR003177; Cytc_oxidase_su7a_met.
DR PANTHER; PTHR10510; PTHR10510; 1.
DR Pfam; PF02238; COX7a; 1.
DR SUPFAM; SSF81419; SSF81419; 1.
PE 1: Evidence at protein level;
KW Acetylation; Direct protein sequencing; Membrane; Mitochondrion;
KW Mitochondrion inner membrane; Oxidoreductase; Reference proteome;
KW Transit peptide; Transmembrane; Transmembrane helix.
FT TRANSIT 1..23
FT /note="Mitochondrion"
FT /evidence="ECO:0000269|PubMed:2175025,
FT ECO:0000269|PubMed:7601105"
FT CHAIN 24..83
FT /note="Cytochrome c oxidase subunit 7A2, mitochondrial"
FT /id="PRO_0000006147"
FT TOPO_DOM 24..48
FT /note="Mitochondrial matrix"
FT /evidence="ECO:0000250|UniProtKB:P14406"
FT TRANSMEM 49..77
FT /note="Helical"
FT /evidence="ECO:0000250|UniProtKB:P07470"
FT TOPO_DOM 78..83
FT /note="Mitochondrial intermembrane"
FT /evidence="ECO:0000250|UniProtKB:P14406"
FT MOD_RES 33
FT /note="N6-acetyllysine"
FT /evidence="ECO:0000250|UniProtKB:P48771"
SQ SEQUENCE 83 AA; 9353 MW; CC0EA163CD47150B CRC64;
MLRNVLALRQ IAQRTISTTS RRHFENKVPE KQKLFQEDNG MPVHLKGGTS DALLYRATML
LTVGGTAYAI YMLAMAAFPK KQN
