CXA4_MOUSE
ID CXA4_MOUSE Reviewed; 333 AA.
AC P28235;
DT 01-DEC-1992, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 2.
DT 03-AUG-2022, entry version 157.
DE RecName: Full=Gap junction alpha-4 protein;
DE AltName: Full=Connexin-37;
DE Short=Cx37;
GN Name=Gja4; Synonyms=Cxn-37;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=1651942; DOI=10.1083/jcb.114.5.1049;
RA Willecke K., Heynkes R., Dahl E., Stutenkemper R., Hennemann H.,
RA Jungbluth S., Suchyna T., Nicholson B.J.;
RT "Mouse connexin37: cloning and functional expression of a gap junction gene
RT highly expressed in lung.";
RL J. Cell Biol. 114:1049-1057(1991).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=129/SvJ; TISSUE=Lung;
RX PubMed=11004519; DOI=10.1016/s0167-4781(00)00122-6;
RA Seul K.H., Beyer E.C.;
RT "Mouse connexin37: gene structure and promoter analysis.";
RL Biochim. Biophys. Acta 1492:499-504(2000).
RN [3]
RP PROTEIN SEQUENCE OF 52-59, AND VARIANTS GLN-54 AND ALA-59.
RC TISSUE=Lung tumor;
RX PubMed=8164742; DOI=10.1038/369067a0;
RA Mandelboim O., Berke G., Fridkin M., Feldman M., Eisenstein M.,
RA Eisenbach L.;
RT "CTL induction by a tumour-associated antigen octapeptide derived from a
RT murine lung carcinoma.";
RL Nature 369:67-71(1994).
CC -!- FUNCTION: One gap junction consists of a cluster of closely packed
CC pairs of transmembrane channels, the connexons, through which materials
CC of low MW diffuse from one cell to a neighboring cell.
CC -!- SUBUNIT: A connexon is composed of a hexamer of connexins.
CC -!- SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. Cell
CC junction, gap junction.
CC -!- TISSUE SPECIFICITY: Highly expressed in lung.
CC -!- SIMILARITY: Belongs to the connexin family. Alpha-type (group II)
CC subfamily. {ECO:0000305}.
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DR EMBL; X57971; CAA41037.1; -; Genomic_DNA.
DR EMBL; AF216832; AAF91221.1; -; mRNA.
DR CCDS; CCDS18667.1; -.
DR PIR; A40548; A40548.
DR RefSeq; NP_032146.1; NM_008120.3.
DR AlphaFoldDB; P28235; -.
DR SMR; P28235; -.
DR STRING; 10090.ENSMUSP00000062320; -.
DR iPTMnet; P28235; -.
DR PhosphoSitePlus; P28235; -.
DR PaxDb; P28235; -.
DR PRIDE; P28235; -.
DR ProteomicsDB; 285406; -.
DR Antibodypedia; 4590; 309 antibodies from 31 providers.
DR DNASU; 14612; -.
DR Ensembl; ENSMUST00000053753; ENSMUSP00000062320; ENSMUSG00000050234.
DR GeneID; 14612; -.
DR KEGG; mmu:14612; -.
DR UCSC; uc008uur.2; mouse.
DR CTD; 2701; -.
DR MGI; MGI:95715; Gja4.
DR VEuPathDB; HostDB:ENSMUSG00000050234; -.
DR eggNOG; ENOG502QU20; Eukaryota.
DR GeneTree; ENSGT01050000244914; -.
DR HOGENOM; CLU_037388_4_2_1; -.
DR InParanoid; P28235; -.
DR OMA; KDPHVER; -.
DR OrthoDB; 903777at2759; -.
DR PhylomeDB; P28235; -.
DR TreeFam; TF329606; -.
DR Reactome; R-MMU-190861; Gap junction assembly.
DR BioGRID-ORCS; 14612; 4 hits in 72 CRISPR screens.
DR ChiTaRS; Gja4; mouse.
DR PRO; PR:P28235; -.
DR Proteomes; UP000000589; Chromosome 4.
DR RNAct; P28235; protein.
DR Bgee; ENSMUSG00000050234; Expressed in primary oocyte and 155 other tissues.
DR ExpressionAtlas; P28235; baseline and differential.
DR Genevisible; P28235; MM.
DR GO; GO:0005922; C:connexin complex; ISO:MGI.
DR GO; GO:0005921; C:gap junction; ISO:MGI.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005243; F:gap junction channel activity; IBA:GO_Central.
DR GO; GO:0001568; P:blood vessel development; IMP:MGI.
DR GO; GO:0006816; P:calcium ion transport; ISO:MGI.
DR GO; GO:0007267; P:cell-cell signaling; ISO:MGI.
DR GO; GO:0003158; P:endothelium development; IEA:Ensembl.
DR GO; GO:0048265; P:response to pain; IEA:Ensembl.
DR DisProt; DP01175; -.
DR Gene3D; 1.20.1440.80; -; 1.
DR InterPro; IPR000500; Connexin.
DR InterPro; IPR002263; Connexin37.
DR InterPro; IPR019570; Connexin_CCC.
DR InterPro; IPR017990; Connexin_CS.
DR InterPro; IPR013092; Connexin_N.
DR InterPro; IPR038359; Connexin_N_sf.
DR PANTHER; PTHR11984; PTHR11984; 1.
DR Pfam; PF00029; Connexin; 1.
DR PRINTS; PR00206; CONNEXIN.
DR PRINTS; PR01134; CONNEXINA4.
DR SMART; SM00037; CNX; 1.
DR SMART; SM01089; Connexin_CCC; 1.
DR PROSITE; PS00407; CONNEXINS_1; 1.
DR PROSITE; PS00408; CONNEXINS_2; 1.
PE 1: Evidence at protein level;
KW Cell junction; Cell membrane; Direct protein sequencing; Gap junction;
KW Membrane; Reference proteome; Transmembrane; Transmembrane helix.
FT CHAIN 1..333
FT /note="Gap junction alpha-4 protein"
FT /id="PRO_0000057815"
FT TOPO_DOM 1..20
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 21..40
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 41..76
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 77..99
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 100..148
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 149..171
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 172..208
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 209..231
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 232..333
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 292..333
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VARIANT 54
FT /note="C -> Q (requires 2 nucleotide substitutions)"
FT /evidence="ECO:0000269|PubMed:8164742"
FT VARIANT 59
FT /note="P -> A"
FT /evidence="ECO:0000269|PubMed:8164742"
SQ SEQUENCE 333 AA; 37596 MW; C73D2B2AC0D926C1 CRC64;
MGDWGFLEKL LDQVQEHSTV VGKIWLTVLF IFRILILGLA GESVWGDEQS DFECNTAQPG
CTNVCYDQAF PISHIRYWVL QFLFVSTPTL IYLGHVIYLS RREERLRQKE GELRALPSKD
LHVERALAAI EHQMAKISVA EDGRLRIRGA LMGTYVVSVL CKSVLEAGFL YGQWRLYGWT
MEPVFVCQRA PCPHIVDCYV SRPTEKTIFI IFMLVVGVIS LVLNLLELVH LLCRCVSREI
KARRDHDARP AQGSASDPYP EQVFFYLPMG EGPSSPPCPT YNGLSSTEQN WANLTTEERL
TSSRPPPFVN TAPQGGRKSP SRPNSSASKK QYV
