CXA5_RAT
ID CXA5_RAT Reviewed; 356 AA.
AC P28234;
DT 01-DEC-1992, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 2.
DT 03-AUG-2022, entry version 155.
DE RecName: Full=Gap junction alpha-5 protein;
DE AltName: Full=Connexin-40;
DE Short=Cx40;
GN Name=Gja5; Synonyms=Cxn-40;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=Sprague-Dawley;
RX PubMed=1370487; DOI=10.1016/s0021-9258(18)46052-8;
RA Haefliger J.-A., Bruzzone R., Jenkins N.A., Gilbert D.J., Copeland N.G.,
RA Paul D.L.;
RT "Four novel members of the connexin family of gap junction proteins.
RT Molecular cloning, expression, and chromosome mapping.";
RL J. Biol. Chem. 267:2057-2064(1992).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=1328644; DOI=10.1007/bf00232759;
RA Beyer E.C., Reed K.E., Westphale E.M., Kanter H.L., Larson D.M.;
RT "Molecular cloning and expression of rat connexin40, a gap junction protein
RT expressed in vascular smooth muscle.";
RL J. Membr. Biol. 127:69-76(1992).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
RX PubMed=9403066; DOI=10.1006/geno.1997.5025;
RA Seul K.H., Tadros P.N., Beyer E.C.;
RT "Mouse connexin40: gene structure and promoter analysis.";
RL Genomics 46:120-126(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Lung;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-351 AND SER-355, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=22673903; DOI=10.1038/ncomms1871;
RA Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C.,
RA Olsen J.V.;
RT "Quantitative maps of protein phosphorylation sites across 14 different rat
RT organs and tissues.";
RL Nat. Commun. 3:876-876(2012).
CC -!- FUNCTION: One gap junction consists of a cluster of closely packed
CC pairs of transmembrane channels, the connexons, through which materials
CC of low MW diffuse from one cell to a neighboring cell.
CC -!- SUBUNIT: A connexon is composed of a hexamer of connexins.
CC -!- SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. Cell
CC junction, gap junction.
CC -!- TISSUE SPECIFICITY: Highly expressed in lung.
CC -!- SIMILARITY: Belongs to the connexin family. Alpha-type (group II)
CC subfamily. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; M76535; AAA41000.1; -; Genomic_DNA.
DR EMBL; M83092; AAA41194.1; -; mRNA.
DR EMBL; AF021806; AAC53502.1; -; mRNA.
DR EMBL; AF022136; AAC53503.1; -; Genomic_DNA.
DR EMBL; BC070935; AAH70935.1; -; mRNA.
DR PIR; A42053; A42053.
DR RefSeq; NP_062153.1; NM_019280.1.
DR PDB; 2K7M; NMR; -; A=252-356.
DR PDBsum; 2K7M; -.
DR AlphaFoldDB; P28234; -.
DR BMRB; P28234; -.
DR SMR; P28234; -.
DR STRING; 10116.ENSRNOP00000023488; -.
DR iPTMnet; P28234; -.
DR PhosphoSitePlus; P28234; -.
DR PaxDb; P28234; -.
DR Ensembl; ENSRNOT00000023488; ENSRNOP00000023488; ENSRNOG00000017484.
DR Ensembl; ENSRNOT00000108116; ENSRNOP00000076921; ENSRNOG00000017484.
DR Ensembl; ENSRNOT00000115241; ENSRNOP00000080221; ENSRNOG00000017484.
DR GeneID; 50563; -.
DR KEGG; rno:50563; -.
DR UCSC; RGD:2692; rat.
DR CTD; 2702; -.
DR RGD; 2692; Gja5.
DR eggNOG; ENOG502QW11; Eukaryota.
DR GeneTree; ENSGT01050000244864; -.
DR HOGENOM; CLU_037388_0_0_1; -.
DR InParanoid; P28234; -.
DR OMA; FIHIHYG; -.
DR OrthoDB; 992551at2759; -.
DR PhylomeDB; P28234; -.
DR TreeFam; TF329606; -.
DR Reactome; R-RNO-190861; Gap junction assembly.
DR EvolutionaryTrace; P28234; -.
DR PRO; PR:P28234; -.
DR Proteomes; UP000002494; Chromosome 2.
DR Bgee; ENSRNOG00000017484; Expressed in lung and 16 other tissues.
DR Genevisible; P28234; RN.
DR GO; GO:0042995; C:cell projection; IDA:RGD.
DR GO; GO:0005922; C:connexin complex; IDA:RGD.
DR GO; GO:0005921; C:gap junction; ISO:RGD.
DR GO; GO:0005887; C:integral component of plasma membrane; ISO:RGD.
DR GO; GO:0014704; C:intercalated disc; ISO:RGD.
DR GO; GO:0071253; F:connexin binding; IPI:RGD.
DR GO; GO:0097718; F:disordered domain specific binding; IPI:CAFA.
DR GO; GO:0005243; F:gap junction channel activity; IDA:RGD.
DR GO; GO:0086076; F:gap junction channel activity involved in atrial cardiac muscle cell-AV node cell electrical coupling; ISO:RGD.
DR GO; GO:0086077; F:gap junction channel activity involved in AV node cell-bundle of His cell electrical coupling; ISO:RGD.
DR GO; GO:0086078; F:gap junction channel activity involved in bundle of His cell-Purkinje myocyte electrical coupling; ISO:RGD.
DR GO; GO:0086075; F:gap junction channel activity involved in cardiac conduction electrical coupling; ISO:RGD.
DR GO; GO:0055077; F:gap junction hemi-channel activity; ISO:RGD.
DR GO; GO:0001525; P:angiogenesis; ISO:RGD.
DR GO; GO:0048844; P:artery morphogenesis; ISO:RGD.
DR GO; GO:0086044; P:atrial cardiac muscle cell to AV node cell communication by electrical coupling; ISO:RGD.
DR GO; GO:0003283; P:atrial septum development; ISO:RGD.
DR GO; GO:0060413; P:atrial septum morphogenesis; ISO:RGD.
DR GO; GO:0003294; P:atrial ventricular junction remodeling; ISO:RGD.
DR GO; GO:0086067; P:AV node cell to bundle of His cell communication; ISO:RGD.
DR GO; GO:0086053; P:AV node cell to bundle of His cell communication by electrical coupling; ISO:RGD.
DR GO; GO:0001568; P:blood vessel development; ISO:RGD.
DR GO; GO:0097746; P:blood vessel diameter maintenance; ISO:RGD.
DR GO; GO:0086054; P:bundle of His cell to Purkinje myocyte communication by electrical coupling; ISO:RGD.
DR GO; GO:0061337; P:cardiac conduction; ISO:RGD.
DR GO; GO:0003161; P:cardiac conduction system development; ISO:RGD.
DR GO; GO:0010643; P:cell communication by chemical coupling; IDA:RGD.
DR GO; GO:0010644; P:cell communication by electrical coupling; IDA:RGD.
DR GO; GO:0086064; P:cell communication by electrical coupling involved in cardiac conduction; ISO:RGD.
DR GO; GO:0086065; P:cell communication involved in cardiac conduction; ISO:RGD.
DR GO; GO:0007267; P:cell-cell signaling; IBA:GO_Central.
DR GO; GO:0035050; P:embryonic heart tube development; ISO:RGD.
DR GO; GO:0030326; P:embryonic limb morphogenesis; ISO:RGD.
DR GO; GO:0003158; P:endothelium development; IEP:RGD.
DR GO; GO:0035922; P:foramen ovale closure; ISO:RGD.
DR GO; GO:0016264; P:gap junction assembly; ISO:RGD.
DR GO; GO:0007507; P:heart development; IEP:RGD.
DR GO; GO:0003174; P:mitral valve development; ISO:RGD.
DR GO; GO:0045776; P:negative regulation of blood pressure; IMP:RGD.
DR GO; GO:0003105; P:negative regulation of glomerular filtration; IMP:RGD.
DR GO; GO:0003151; P:outflow tract morphogenesis; ISO:RGD.
DR GO; GO:0010652; P:positive regulation of cell communication by chemical coupling; IMP:RGD.
DR GO; GO:0045907; P:positive regulation of vasoconstriction; IMP:RGD.
DR GO; GO:0006813; P:potassium ion transport; IDA:RGD.
DR GO; GO:0003193; P:pulmonary valve formation; ISO:RGD.
DR GO; GO:0098910; P:regulation of atrial cardiac muscle cell action potential; ISO:RGD.
DR GO; GO:0060371; P:regulation of atrial cardiac muscle cell membrane depolarization; ISO:RGD.
DR GO; GO:0098904; P:regulation of AV node cell action potential; ISO:RGD.
DR GO; GO:0098905; P:regulation of bundle of His cell action potential; ISO:RGD.
DR GO; GO:0055117; P:regulation of cardiac muscle contraction; ISO:RGD.
DR GO; GO:0010649; P:regulation of cell communication by electrical coupling; IDA:RGD.
DR GO; GO:0086091; P:regulation of heart rate by cardiac conduction; ISO:RGD.
DR GO; GO:1900825; P:regulation of membrane depolarization during cardiac muscle cell action potential; ISO:RGD.
DR GO; GO:0098906; P:regulation of Purkinje myocyte action potential; ISO:RGD.
DR GO; GO:1900133; P:regulation of renin secretion into blood stream; ISO:RGD.
DR GO; GO:0003073; P:regulation of systemic arterial blood pressure; ISO:RGD.
DR GO; GO:0060373; P:regulation of ventricular cardiac muscle cell membrane depolarization; ISO:RGD.
DR GO; GO:0060307; P:regulation of ventricular cardiac muscle cell membrane repolarization; ISO:RGD.
DR GO; GO:0003071; P:renal system process involved in regulation of systemic arterial blood pressure; ISO:RGD.
DR GO; GO:0086015; P:SA node cell action potential; ISO:RGD.
DR GO; GO:0003284; P:septum primum development; ISO:RGD.
DR GO; GO:0001501; P:skeletal system development; ISO:RGD.
DR GO; GO:0042311; P:vasodilation; IMP:RGD.
DR GO; GO:1990029; P:vasomotion; IMP:RGD.
DR GO; GO:0086005; P:ventricular cardiac muscle cell action potential; ISO:RGD.
DR GO; GO:0003281; P:ventricular septum development; ISO:RGD.
DR GO; GO:0060412; P:ventricular septum morphogenesis; ISO:RGD.
DR Gene3D; 1.20.1440.80; -; 1.
DR InterPro; IPR000500; Connexin.
DR InterPro; IPR002264; Connexin40.
DR InterPro; IPR034634; Connexin_C.
DR InterPro; IPR019570; Connexin_CCC.
DR InterPro; IPR017990; Connexin_CS.
DR InterPro; IPR013092; Connexin_N.
DR InterPro; IPR038359; Connexin_N_sf.
DR InterPro; IPR031862; Cx40_C.
DR PANTHER; PTHR11984; PTHR11984; 1.
DR PANTHER; PTHR11984:SF13; PTHR11984:SF13; 1.
DR Pfam; PF00029; Connexin; 1.
DR Pfam; PF16791; Connexin40_C; 1.
DR PRINTS; PR00206; CONNEXIN.
DR PRINTS; PR01135; CONNEXINA5.
DR SMART; SM00037; CNX; 1.
DR SMART; SM01089; Connexin_CCC; 1.
DR SUPFAM; SSF118220; SSF118220; 1.
DR PROSITE; PS00407; CONNEXINS_1; 1.
DR PROSITE; PS00408; CONNEXINS_2; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cell junction; Cell membrane; Gap junction; Membrane;
KW Phosphoprotein; Reference proteome; Transmembrane; Transmembrane helix.
FT CHAIN 1..356
FT /note="Gap junction alpha-5 protein"
FT /id="PRO_0000057821"
FT TOPO_DOM 1..19
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 20..40
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 41..76
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 77..97
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 98..163
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 164..184
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 185..204
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 205..225
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 226..356
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 241..266
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 279..356
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 246..266
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 333..356
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 351
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:22673903"
FT MOD_RES 355
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:22673903"
FT STRAND 261..263
FT /evidence="ECO:0007829|PDB:2K7M"
FT TURN 266..269
FT /evidence="ECO:0007829|PDB:2K7M"
FT STRAND 293..297
FT /evidence="ECO:0007829|PDB:2K7M"
FT STRAND 300..303
FT /evidence="ECO:0007829|PDB:2K7M"
FT STRAND 323..325
FT /evidence="ECO:0007829|PDB:2K7M"
FT STRAND 327..329
FT /evidence="ECO:0007829|PDB:2K7M"
FT TURN 332..334
FT /evidence="ECO:0007829|PDB:2K7M"
SQ SEQUENCE 356 AA; 40233 MW; 7D459FE69D9ACF0C CRC64;
MGDWSFLGEF LEEVHKHSTV IGKVWLTVLF IFRMLVLGTA AESSWGDEQA DFRCDTIQPG
CQNVCYDQAF PISHIRYWVL QIIFVSTPSL VYMGHAMHTV RMQEKQKLRE AEKAKEAGGT
GTYEYLAEKA ELSCWKEVNG KIVLQGTLLN TYVCTILIRT AMEVAFMVGQ YLLYGIFLDT
LHVCRRSPCP HPVNCYVSRP TEKNVFIVFM MAVAGLSLFL SLAELYHLGW KKIRQRLAKS
RQGDKHQLLG PSTSLVQGLT PPPDFNQCLK NSPDEKFFSD FSNNMGSRKN PDPLATEEVP
NQEQIPEEGF IHTQYGQKPE QPSGASAGHR FPQGYHSDKR RLSKASSKAR SDDLSV
