CXA6_MOUSE
ID CXA6_MOUSE Reviewed; 283 AA.
AC Q6S5G4; Q3TFY9; Q8BLZ9;
DT 10-MAY-2005, integrated into UniProtKB/Swiss-Prot.
DT 03-OCT-2012, sequence version 2.
DT 03-AUG-2022, entry version 115.
DE RecName: Full=Gap junction alpha-6 protein;
DE AltName: Full=Connexin-33;
DE Short=Cx33;
GN Name=Gja6;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=C57BL/6 X DBA/2; TISSUE=Testis;
RA Gillot I., Ranc F., Martin L.;
RT "A critical role of the mouse connexin 33 in Sertoli-germ cells
RT interactions.";
RL Submitted (NOV-2003) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Aorta, Liver, and Vein;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: One gap junction consists of a cluster of closely packed
CC pairs of transmembrane channels, the connexons, through which materials
CC of low MW diffuse from one cell to a neighboring cell.
CC -!- SUBUNIT: A connexon is composed of a hexamer of connexins.
CC -!- SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. Cell
CC junction, gap junction.
CC -!- SIMILARITY: Belongs to the connexin family. Alpha-type (group II)
CC subfamily. {ECO:0000305}.
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DR EMBL; AY465183; AAR28037.1; -; Genomic_DNA.
DR EMBL; AK040693; BAC30669.1; -; mRNA.
DR EMBL; AK146838; BAE27472.1; -; mRNA.
DR EMBL; AK168953; BAE40759.1; -; mRNA.
DR EMBL; AL669939; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AL670462; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CR478402; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC117760; AAI17761.1; -; mRNA.
DR EMBL; CH466571; EDL40800.1; -; Genomic_DNA.
DR CCDS; CCDS30506.1; -.
DR RefSeq; NP_001001496.1; NM_001001496.2.
DR AlphaFoldDB; Q6S5G4; -.
DR SMR; Q6S5G4; -.
DR STRING; 10090.ENSMUSP00000069187; -.
DR PaxDb; Q6S5G4; -.
DR PRIDE; Q6S5G4; -.
DR Antibodypedia; 4382; 1230 antibodies from 49 providers.
DR Ensembl; ENSMUST00000069417; ENSMUSP00000069187; ENSMUSG00000055691.
DR GeneID; 414089; -.
DR KEGG; mmu:414089; -.
DR UCSC; uc009utt.2; mouse.
DR CTD; 414089; -.
DR MGI; MGI:95717; Gja6.
DR VEuPathDB; HostDB:ENSMUSG00000055691; -.
DR eggNOG; ENOG502QRAE; Eukaryota.
DR GeneTree; ENSGT01050000244914; -.
DR HOGENOM; CLU_037388_4_1_1; -.
DR InParanoid; Q6S5G4; -.
DR OrthoDB; 1172752at2759; -.
DR PhylomeDB; Q6S5G4; -.
DR TreeFam; TF329606; -.
DR BioGRID-ORCS; 414089; 1 hit in 70 CRISPR screens.
DR PRO; PR:Q6S5G4; -.
DR Proteomes; UP000000589; Chromosome X.
DR RNAct; Q6S5G4; protein.
DR Bgee; ENSMUSG00000055691; Expressed in spermatocyte and 13 other tissues.
DR ExpressionAtlas; Q6S5G4; baseline and differential.
DR GO; GO:0030054; C:cell junction; ISO:MGI.
DR GO; GO:0044291; C:cell-cell contact zone; ISO:MGI.
DR GO; GO:0005922; C:connexin complex; IEA:InterPro.
DR GO; GO:0005737; C:cytoplasm; ISO:MGI.
DR GO; GO:0005921; C:gap junction; ISO:MGI.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0014704; C:intercalated disc; ISO:MGI.
DR GO; GO:0043231; C:intracellular membrane-bounded organelle; ISO:MGI.
DR GO; GO:0005739; C:mitochondrion; ISO:MGI.
DR GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR GO; GO:0005634; C:nucleus; ISO:MGI.
DR GO; GO:0005886; C:plasma membrane; ISO:MGI.
DR GO; GO:0070160; C:tight junction; ISO:MGI.
DR GO; GO:0043014; F:alpha-tubulin binding; ISO:MGI.
DR GO; GO:0008013; F:beta-catenin binding; ISO:MGI.
DR GO; GO:0005243; F:gap junction channel activity; ISO:MGI.
DR GO; GO:1903763; F:gap junction channel activity involved in cell communication by electrical coupling; ISO:MGI.
DR GO; GO:0055077; F:gap junction hemi-channel activity; ISO:MGI.
DR GO; GO:0015075; F:ion transmembrane transporter activity; ISO:MGI.
DR GO; GO:0015631; F:tubulin binding; ISO:MGI.
DR GO; GO:0007267; P:cell-cell signaling; IBA:GO_Central.
DR Gene3D; 1.20.1440.80; -; 1.
DR InterPro; IPR000500; Connexin.
DR InterPro; IPR019570; Connexin_CCC.
DR InterPro; IPR017990; Connexin_CS.
DR InterPro; IPR013092; Connexin_N.
DR InterPro; IPR038359; Connexin_N_sf.
DR PANTHER; PTHR11984; PTHR11984; 1.
DR Pfam; PF00029; Connexin; 1.
DR PRINTS; PR00206; CONNEXIN.
DR SMART; SM00037; CNX; 1.
DR SMART; SM01089; Connexin_CCC; 1.
DR PROSITE; PS00407; CONNEXINS_1; 1.
DR PROSITE; PS00408; CONNEXINS_2; 1.
PE 2: Evidence at transcript level;
KW Cell junction; Cell membrane; Gap junction; Membrane; Reference proteome;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..283
FT /note="Gap junction alpha-6 protein"
FT /id="PRO_0000057823"
FT TOPO_DOM 1..23
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 24..41
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 42..76
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 77..99
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 100..150
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 151..173
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 174..208
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 209..231
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 232..283
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT CONFLICT 96
FT /note="V -> A (in Ref. 1; AAR28037)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 283 AA; 32660 MW; C5CF01833AF02B4F CRC64;
MSDWSALHQL LEKVQPYSTA GGKVWIKVLF IFRILLLGTA IESAWSDEQF EFHCNTQQPG
CENVCYDHAF PISHVRLWVL QVIFVSVPIL LYLAHVYYVV RQNKKLNKQE EELEAAHFNE
ASVERHLETI AGEQFKCGSE EQSKVKMRGR LLLTYMASIF FKSVFEMAFL LIQWYIYGFT
LSALYICEQS PCPRRVDCFL SRPTEKTIFI LFMFVVSVVS FVLDIIELFY VLFKAIKNRM
RKAEDEVYCD ELPCPSHVSS STVLTTIDSS EQAVPVELSS VCI
