ID   CXAT1_CONMS             Reviewed;          93 AA.
AC   C4PWC3;
DT   02-MAR-2010, integrated into UniProtKB/Swiss-Prot.
DT   07-JUL-2009, sequence version 1.
DT   25-MAY-2022, entry version 31.
DE   RecName: Full=Alpha-conotoxin-like Ms20.1;
DE   AltName: Full=MsXXA;
DE   Flags: Precursor;
OS   Conus mustelinus (Weasel cone).
OC   Eukaryota; Metazoa; Spiralia; Lophotrochozoa; Mollusca; Gastropoda;
OC   Caenogastropoda; Neogastropoda; Conoidea; Conidae; Conus; Rhizoconus.
OX   NCBI_TaxID=101309;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], IDENTIFICATION BY MASS SPECTROMETRY, AND
RP   SUBUNIT.
RC   TISSUE=Venom, and Venom duct;
RX   PubMed=19275168; DOI=10.1021/bi9000326;
RA   Loughnan M.L., Nicke A., Lawrence N., Lewis R.J.;
RT   "Novel alpha D-conopeptides and their precursors identified by cDNA cloning
RT   define the D-conotoxin superfamily.";
RL   Biochemistry 48:3717-3729(2009).
CC   -!- FUNCTION: Alpha-conotoxins act on postsynaptic membranes, they bind to
CC       the nicotinic acetylcholine receptors (nAChR) and thus inhibit them.
CC       This toxin specifically blocks mammalian neuronal nAChR of the alpha-
CC       7/CHRNA7, alpha-3-beta-2/CHRNA3-CHRNB2 and alpha-4-beta-2/CHRNA4-CHRNB2
CC       subtypes (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Hetero-, homo- or pseudo-homodimers (identical sequence,
CC       different post-translational modifications) (By similarity).
CC       Heterodimer of [carboxyGlu-48, hydroxyPro-54]Ms20.1 and [carboxy'Glu-
CC       50', hydroxy'Pro-56']Ms20.4 may exist. {ECO:0000250,
CC       ECO:0000269|PubMed:19275168}.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom duct.
CC   -!- DOMAIN: The cysteine framework is XX (C-CC-C-CC-C-C-C-C).
CC   -!- PTM: Contains 5 disulfide bonds. {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the conotoxin D superfamily. {ECO:0000305}.
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DR   EMBL; FN178635; CAX51121.1; -; mRNA.
DR   AlphaFoldDB; C4PWC3; -.
DR   SMR; C4PWC3; -.
DR   ConoServer; 3596; Ms20.1 precursor.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0035792; C:host cell postsynaptic membrane; IEA:UniProtKB-KW.
DR   GO; GO:0030550; F:acetylcholine receptor inhibitor activity; IEA:UniProtKB-KW.
DR   GO; GO:0099106; F:ion channel regulator activity; IEA:UniProtKB-KW.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Acetylcholine receptor inhibiting toxin; Disulfide bond;
KW   Gamma-carboxyglutamic acid; Hydroxylation; Ion channel impairing toxin;
KW   Neurotoxin; Postsynaptic neurotoxin; Secreted; Signal; Toxin.
FT   SIGNAL          1..23
FT                   /evidence="ECO:0000255"
FT   PROPEP          24..45
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_5000467220"
FT   CHAIN           46..93
FT                   /note="Alpha-conotoxin-like Ms20.1"
FT                   /id="PRO_5000467221"
FT   MOD_RES         48
FT                   /note="4-carboxyglutamate"
FT                   /evidence="ECO:0000250"
FT   MOD_RES         54
FT                   /note="4-hydroxyproline"
FT                   /evidence="ECO:0000250"
FT   VARIANT         2
FT                   /note="L -> P"
SQ   SEQUENCE   93 AA;  10507 MW;  0A3EF707DD046DA6 CRC64;
     MLKLEMMLVV LLILPLFYFD AGGQVVQRDW RSDGLARYLQ RGDRDVRECN INTPGSSWGK
     CCLTRMCGPM CCARSGCTCV YHWRRGHGCS CPG