CXB6_MOUSE
ID CXB6_MOUSE Reviewed; 261 AA.
AC P70689;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1997, sequence version 1.
DT 03-AUG-2022, entry version 156.
DE RecName: Full=Gap junction beta-6 protein;
DE AltName: Full=Connexin-30;
DE Short=Cx30;
GN Name=Gjb6; Synonyms=Cxn-30;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=8663509; DOI=10.1074/jbc.271.30.17903;
RA Dahl E., Manthey D., Chen Y., Schwarz H.-J., Chang Y.S., Lalley P.A.,
RA Nicholson B.J., Willecke K.;
RT "Molecular cloning and functional expression of mouse connexin-30, a gap
RT junction gene highly expressed in adult brain and skin.";
RL J. Biol. Chem. 271:17903-17910(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Bone, and Mammary gland;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP INTERACTION WITH CNST.
RX PubMed=19864490; DOI=10.1093/hmg/ddp490;
RA del Castillo F.J., Cohen-Salmon M., Charollais A., Caille D., Lampe P.D.,
RA Chavrier P., Meda P., Petit C.;
RT "Consortin, a trans-Golgi network cargo receptor for the plasma membrane
RT targeting and recycling of connexins.";
RL Hum. Mol. Genet. 19:262-275(2010).
CC -!- FUNCTION: One gap junction consists of a cluster of closely packed
CC pairs of transmembrane channels, the connexons, through which materials
CC of low MW diffuse from one cell to a neighboring cell.
CC -!- SUBUNIT: A connexon is composed of a hexamer of connexins. Interacts
CC with CNST. {ECO:0000269|PubMed:19864490}.
CC -!- INTERACTION:
CC P70689; P39447: Tjp1; NbExp=3; IntAct=EBI-2615416, EBI-79508;
CC -!- SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. Cell
CC junction, gap junction.
CC -!- TISSUE SPECIFICITY: Highly expressed in adult brain and skin. Less in
CC uterus, lung and eye. Very low in testis and sciatic nerve. No
CC expression before birth.
CC -!- SIMILARITY: Belongs to the connexin family. Beta-type (group I)
CC subfamily. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; Z70023; CAA93845.1; -; Genomic_DNA.
DR EMBL; BC013811; AAH13811.1; -; mRNA.
DR EMBL; BC016507; AAH16507.1; -; mRNA.
DR EMBL; BC063762; AAH63762.1; -; mRNA.
DR CCDS; CCDS27154.1; -.
DR RefSeq; NP_001010937.1; NM_001010937.2.
DR RefSeq; NP_001258592.1; NM_001271663.1.
DR RefSeq; XP_006518623.1; XM_006518560.2.
DR RefSeq; XP_006518624.1; XM_006518561.3.
DR AlphaFoldDB; P70689; -.
DR SMR; P70689; -.
DR BioGRID; 199936; 4.
DR IntAct; P70689; 2.
DR MINT; P70689; -.
DR STRING; 10090.ENSMUSP00000035630; -.
DR iPTMnet; P70689; -.
DR PhosphoSitePlus; P70689; -.
DR PaxDb; P70689; -.
DR PRIDE; P70689; -.
DR ProteomicsDB; 279219; -.
DR Antibodypedia; 6442; 249 antibodies from 29 providers.
DR DNASU; 14623; -.
DR Ensembl; ENSMUST00000039380; ENSMUSP00000035630; ENSMUSG00000040055.
DR GeneID; 14623; -.
DR KEGG; mmu:14623; -.
DR UCSC; uc007ucy.2; mouse.
DR CTD; 10804; -.
DR MGI; MGI:107588; Gjb6.
DR VEuPathDB; HostDB:ENSMUSG00000040055; -.
DR eggNOG; ENOG502QWM8; Eukaryota.
DR GeneTree; ENSGT01030000234513; -.
DR HOGENOM; CLU_037388_4_1_1; -.
DR InParanoid; P70689; -.
DR OMA; PNHAIKE; -.
DR OrthoDB; 1043502at2759; -.
DR PhylomeDB; P70689; -.
DR TreeFam; TF329606; -.
DR Reactome; R-MMU-190861; Gap junction assembly.
DR BioGRID-ORCS; 14623; 0 hits in 73 CRISPR screens.
DR PRO; PR:P70689; -.
DR Proteomes; UP000000589; Chromosome 14.
DR RNAct; P70689; protein.
DR Bgee; ENSMUSG00000040055; Expressed in utricle of membranous labyrinth and 136 other tissues.
DR ExpressionAtlas; P70689; baseline and differential.
DR Genevisible; P70689; MM.
DR GO; GO:0005884; C:actin filament; ISO:MGI.
DR GO; GO:0016324; C:apical plasma membrane; ISO:MGI.
DR GO; GO:0030054; C:cell junction; ISO:MGI.
DR GO; GO:0005922; C:connexin complex; IEA:InterPro.
DR GO; GO:0005829; C:cytosol; ISO:MGI.
DR GO; GO:0005921; C:gap junction; IDA:MGI.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0051015; F:actin filament binding; ISO:MGI.
DR GO; GO:0048487; F:beta-tubulin binding; ISO:MGI.
DR GO; GO:0005243; F:gap junction channel activity; ISO:MGI.
DR GO; GO:1903763; F:gap junction channel activity involved in cell communication by electrical coupling; ISO:MGI.
DR GO; GO:0008017; F:microtubule binding; ISO:MGI.
DR GO; GO:0007568; P:aging; IEA:Ensembl.
DR GO; GO:0006915; P:apoptotic process; IEA:Ensembl.
DR GO; GO:0007267; P:cell-cell signaling; IBA:GO_Central.
DR GO; GO:0071333; P:cellular response to glucose stimulus; IEA:Ensembl.
DR GO; GO:0042471; P:ear morphogenesis; IMP:MGI.
DR GO; GO:0016264; P:gap junction assembly; ISO:MGI.
DR GO; GO:1990349; P:gap junction-mediated intercellular transport; ISO:MGI.
DR GO; GO:0048839; P:inner ear development; IEA:Ensembl.
DR GO; GO:0008285; P:negative regulation of cell population proliferation; ISO:MGI.
DR GO; GO:0051602; P:response to electrical stimulus; IEA:Ensembl.
DR GO; GO:0032496; P:response to lipopolysaccharide; IEA:Ensembl.
DR GO; GO:0007605; P:sensory perception of sound; IMP:MGI.
DR GO; GO:0055085; P:transmembrane transport; ISO:MGI.
DR Gene3D; 1.20.1440.80; -; 1.
DR InterPro; IPR000500; Connexin.
DR InterPro; IPR019570; Connexin_CCC.
DR InterPro; IPR017990; Connexin_CS.
DR InterPro; IPR013092; Connexin_N.
DR InterPro; IPR038359; Connexin_N_sf.
DR PANTHER; PTHR11984; PTHR11984; 1.
DR Pfam; PF00029; Connexin; 1.
DR PRINTS; PR00206; CONNEXIN.
DR SMART; SM00037; CNX; 1.
DR SMART; SM01089; Connexin_CCC; 1.
DR PROSITE; PS00407; CONNEXINS_1; 1.
DR PROSITE; PS00408; CONNEXINS_2; 1.
PE 1: Evidence at protein level;
KW Cell junction; Cell membrane; Gap junction; Hearing; Membrane;
KW Reference proteome; Transmembrane; Transmembrane helix.
FT CHAIN 1..261
FT /note="Gap junction beta-6 protein"
FT /id="PRO_0000057872"
FT TOPO_DOM 1..22
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 23..45
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 46..75
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 76..98
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 99..131
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 132..154
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 155..192
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 193..215
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 216..261
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
SQ SEQUENCE 261 AA; 30366 MW; AC6D8AA234AA47A6 CRC64;
MDWGTLHTVI GGVNKHSTSI GKVWITVIFI FRVMILVVAA QEVWGDEQED FVCNTLQPGC
KNVCYDHFFP VSHIRLWALQ LIFVSTPALL VAMHVAYYRH ETARKFIRGE KRNEFKDLED
IKRQKVRIEG SLWWTYTSSI FFRIIFEAAF MYVFYFLYNG YHLPWVLKCG IDPCPNLVDC
FISRPTEKTV FTVFMISASV ICMLLNVAEL CYLLLKLCFR RSKRTQAQRN HPNHALKESK
QNEMNELISD SGQNAITSFP S
