CXCL2_RAT
ID CXCL2_RAT Reviewed; 100 AA.
AC P30348; Q5WA60;
DT 01-APR-1993, integrated into UniProtKB/Swiss-Prot.
DT 01-APR-1993, sequence version 1.
DT 03-AUG-2022, entry version 145.
DE RecName: Full=C-X-C motif chemokine 2;
DE AltName: Full=Cytokine-induced neutrophil chemoattractant 3;
DE Short=CINC-3;
DE AltName: Full=Macrophage inflammatory protein 2;
DE Short=MIP2;
DE Flags: Precursor;
GN Name=Cxcl2; Synonyms=Cinc3, Mip-2, Mip2, Scyb2;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=Fischer 344; TISSUE=Lung;
RX PubMed=7665992; DOI=10.1002/jlb.58.3.359;
RA Driscoll K.E., Hassenbein D.G., Howard B.W., Isfort R.J., Cody D.,
RA Tindal M.H., Suchanek M., Carter J.M.;
RT "Cloning, expression, and functional characterization of rat MIP-2: a
RT neutrophil chemoattractant and epithelial cell mitogen.";
RL J. Leukoc. Biol. 58:359-364(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=Fischer;
RX PubMed=7883948; DOI=10.1172/jci117745;
RA Feng L., Xia Y., Yoshimura T., Wilson C.B.;
RT "Modulation of neutrophil influx in glomerulonephritis in the rat with
RT anti-macrophage inflammatory protein-2 (MIP-2) antibody.";
RL J. Clin. Invest. 95:1009-1017(1995).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=CD Charles River; TISSUE=Lung;
RA Farone A., Farone M., Shi M.M., Kobzik L., Paulauskis J.D.;
RT "Molecular cloning and mRNA expression of rat macrophage inflammatory
RT protein-2.";
RL Submitted (MAR-1996) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=Sprague-Dawley;
RX PubMed=14624457; DOI=10.1002/jcb.10710;
RA Numahata K., Komagata T., Hirasawa N., Someya K., Xiao Y.Q., Ohuchi K.;
RT "Analysis of the mechanism regulating the stability of rat macrophage
RT inflammatory protein-2 mRNA in RBL-2H3 cells.";
RL J. Cell. Biochem. 90:976-986(2003).
RN [5]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 39-91.
RC TISSUE=Lung;
RX PubMed=1415488;
RA Huang S., Paulauskis J.D., Godleski J.J., Kobzik L.;
RT "Expression of macrophage inflammatory protein-2 and KC mRNA in pulmonary
RT inflammation.";
RL Am. J. Pathol. 141:981-988(1992).
RN [6]
RP PROTEIN SEQUENCE OF 32-100.
RC STRAIN=Wistar;
RX PubMed=8043001; DOI=10.1042/bj3010545;
RA Nakagawa H., Komorita N., Shibata F., Ikesue A., Konishi K., Fujioka M.,
RA Kato H.;
RT "Identification of cytokine-induced neutrophil chemoattractants (CINC), rat
RT GRO/CINC-2 alpha and CINC-2 beta, produced by granulation tissue in
RT culture: purification, complete amino acid sequences and
RT characterization.";
RL Biochem. J. 301:545-550(1994).
RN [7]
RP PROTEIN SEQUENCE OF 32-59.
RC STRAIN=Wistar;
RX PubMed=8607872; DOI=10.1006/bbrc.1996.0511;
RA Nakagawa H., Shiota S., Takano K., Shibata F., Kato H.;
RT "Cytokine-induced neutrophil chemoattractant (CINC)-2 alpha, a novel member
RT of rat GRO/CINCs, is a predominant chemokine produced by
RT lipopolysaccharide-stimulated rat macrophages in culture.";
RL Biochem. Biophys. Res. Commun. 220:945-948(1996).
RN [8]
RP PROTEIN SEQUENCE OF 32-45.
RX PubMed=8471066; DOI=10.1016/0006-2952(93)90041-t;
RA Nakagawa H., Ikesue A., Hatakeyama S., Kato H., Gotoda T., Komorita N.,
RA Watanabe K., Miyai H.;
RT "Production of an interleukin-8-like chemokine by cytokine-stimulated rat
RT NRK-49F fibroblasts and its suppression by anti-inflammatory steroids.";
RL Biochem. Pharmacol. 45:1425-1430(1993).
CC -!- FUNCTION: Chemotactic for human polymorphonuclear leukocytes but does
CC not induce chemokinesis or an oxidative burst. Contributes to
CC neutrophil activation during inflammation.
CC -!- SUBUNIT: Homotetramer.
CC -!- SUBCELLULAR LOCATION: Secreted.
CC -!- TISSUE SPECIFICITY: At least expressed in the lung and trachea.
CC -!- INDUCTION: By lipopolysaccharide (LPS) and inflammation; in lung.
CC -!- SIMILARITY: Belongs to the intercrine alpha (chemokine CxC) family.
CC {ECO:0000305}.
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DR EMBL; X65647; CAA46599.1; -; mRNA.
DR EMBL; S77604; AAB33749.1; -; mRNA.
DR EMBL; U45965; AAA92438.1; -; mRNA.
DR EMBL; AB060092; BAB41105.1; -; mRNA.
DR EMBL; S45855; -; NOT_ANNOTATED_CDS; mRNA.
DR PIR; S21467; S21467.
DR RefSeq; NP_446099.1; NM_053647.1.
DR AlphaFoldDB; P30348; -.
DR SMR; P30348; -.
DR BioGRID; 250284; 1.
DR STRING; 10116.ENSRNOP00000003745; -.
DR PaxDb; P30348; -.
DR Ensembl; ENSRNOT00000003745; ENSRNOP00000003745; ENSRNOG00000002792.
DR GeneID; 114105; -.
DR KEGG; rno:114105; -.
DR UCSC; RGD:70069; rat.
DR CTD; 2920; -.
DR RGD; 70069; Cxcl2.
DR eggNOG; ENOG502S7MM; Eukaryota.
DR GeneTree; ENSGT00940000155233; -.
DR HOGENOM; CLU_143902_1_0_1; -.
DR InParanoid; P30348; -.
DR OMA; QPEVIAM; -.
DR OrthoDB; 1617719at2759; -.
DR PhylomeDB; P30348; -.
DR TreeFam; TF333433; -.
DR Reactome; R-RNO-380108; Chemokine receptors bind chemokines.
DR Reactome; R-RNO-418594; G alpha (i) signalling events.
DR Reactome; R-RNO-6798695; Neutrophil degranulation.
DR PRO; PR:P30348; -.
DR Proteomes; UP000002494; Chromosome 14.
DR Bgee; ENSRNOG00000002792; Expressed in stomach and 5 other tissues.
DR Genevisible; P30348; RN.
DR GO; GO:0005615; C:extracellular space; IDA:RGD.
DR GO; GO:0008009; F:chemokine activity; IDA:RGD.
DR GO; GO:0045236; F:CXCR chemokine receptor binding; IBA:GO_Central.
DR GO; GO:0061844; P:antimicrobial humoral immune response mediated by antimicrobial peptide; IBA:GO_Central.
DR GO; GO:0071347; P:cellular response to interleukin-1; IEP:RGD.
DR GO; GO:0071222; P:cellular response to lipopolysaccharide; IEP:RGD.
DR GO; GO:0070098; P:chemokine-mediated signaling pathway; IBA:GO_Central.
DR GO; GO:0006954; P:inflammatory response; IBA:GO_Central.
DR GO; GO:0030595; P:leukocyte chemotaxis; IMP:RGD.
DR GO; GO:0070997; P:neuron death; IDA:RGD.
DR GO; GO:0030593; P:neutrophil chemotaxis; IMP:RGD.
DR GO; GO:0007204; P:positive regulation of cytosolic calcium ion concentration; IDA:RGD.
DR GO; GO:0001975; P:response to amphetamine; IEP:RGD.
DR GO; GO:0032355; P:response to estradiol; IEP:RGD.
DR GO; GO:0010332; P:response to gamma radiation; IEP:RGD.
DR GO; GO:0051384; P:response to glucocorticoid; IEP:RGD.
DR GO; GO:0009408; P:response to heat; IEP:RGD.
DR GO; GO:0002237; P:response to molecule of bacterial origin; ISO:RGD.
DR GO; GO:0042060; P:wound healing; IEP:RGD.
DR CDD; cd00273; Chemokine_CXC; 1.
DR InterPro; IPR001089; Chemokine_CXC.
DR InterPro; IPR018048; Chemokine_CXC_CS.
DR InterPro; IPR001811; Chemokine_IL8-like_dom.
DR InterPro; IPR033899; CXC_Chemokine_domain.
DR InterPro; IPR036048; Interleukin_8-like_sf.
DR PANTHER; PTHR10179; PTHR10179; 1.
DR Pfam; PF00048; IL8; 1.
DR PRINTS; PR00437; SMALLCYTKCXC.
DR SMART; SM00199; SCY; 1.
DR SUPFAM; SSF54117; SSF54117; 1.
DR PROSITE; PS00471; SMALL_CYTOKINES_CXC; 1.
PE 1: Evidence at protein level;
KW Chemotaxis; Cytokine; Direct protein sequencing; Disulfide bond;
KW Inflammatory response; Reference proteome; Secreted; Signal.
FT SIGNAL 1..31
FT /evidence="ECO:0000269|PubMed:8043001,
FT ECO:0000269|PubMed:8471066, ECO:0000269|PubMed:8607872"
FT CHAIN 32..100
FT /note="C-X-C motif chemokine 2"
FT /id="PRO_0000005062"
FT DISULFID 36..62
FT /evidence="ECO:0000250"
FT DISULFID 38..78
FT /evidence="ECO:0000250"
SQ SEQUENCE 100 AA; 10783 MW; 4299DFE68FF37A9B CRC64;
MAPPTRQLLN AVLVLLLLLA TNHQGTGVVV ASELRCQCLT TLPRVDFKNI QSLTVTPPGP
HCAQTEVIAT LKDGHEVCLN PEAPLVQRIV QKILNKGKAN
