CXCL3_RAT
ID CXCL3_RAT Reviewed; 101 AA.
AC Q10746; Q10747; Q5CZ55; Q9EP62;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 25-NOV-2008, sequence version 2.
DT 03-AUG-2022, entry version 126.
DE RecName: Full=C-X-C motif chemokine 3;
DE AltName: Full=Cytokine-induced neutrophil chemoattractant 2;
DE Short=CINC-2;
DE AltName: Full=Macrophage inflammatory protein 2-alpha/beta;
DE Short=MIP2-alpha/beta;
DE Flags: Precursor;
GN Name=Cxcl3; Synonyms=Cinc2;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), PROTEIN SEQUENCE OF 33-100 (ISOFORM
RP 1), PROTEIN SEQUENCE OF 33-67 (ISOFORM 2), AND FUNCTION.
RC STRAIN=Wistar; TISSUE=Peritoneal cavity;
RX PubMed=8043001; DOI=10.1042/bj3010545;
RA Nakagawa H., Komorita N., Shibata F., Ikesue A., Konishi K., Fujioka M.,
RA Kato H.;
RT "Identification of cytokine-induced neutrophil chemoattractants (CINC), rat
RT GRO/CINC-2 alpha and CINC-2 beta, produced by granulation tissue in
RT culture: purification, complete amino acid sequences and
RT characterization.";
RL Biochem. J. 301:545-550(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORM 1), NUCLEOTIDE SEQUENCE
RP [GENOMIC DNA] (ISOFORM 2), AND INDUCTION.
RC STRAIN=Wistar; TISSUE=Peritoneal cavity;
RX PubMed=9576061; DOI=10.1006/cyto.1997.0271;
RA Shibata F., Konishi K., Nakagawa H.;
RT "Gene structure, cDNA cloning, and expression of the rat cytokine-induced
RT neutrophil chemoattractant-2 (CINC-2) gene.";
RL Cytokine 10:169-174(1998).
RN [3]
RP PROTEIN SEQUENCE OF 33-56.
RC TISSUE=Fibroblast;
RX PubMed=8471066; DOI=10.1016/0006-2952(93)90041-t;
RA Nakagawa H., Ikesue A., Hatakeyama S., Kato H., Gotoda T., Komorita N.,
RA Watanabe K., Miyai H.;
RT "Production of an interleukin-8-like chemokine by cytokine-stimulated rat
RT NRK-49F fibroblasts and its suppression by anti-inflammatory steroids.";
RL Biochem. Pharmacol. 45:1425-1430(1993).
RN [4]
RP PROTEIN SEQUENCE OF 33-61, AND FUNCTION.
RC STRAIN=Wistar;
RX PubMed=8607872; DOI=10.1006/bbrc.1996.0511;
RA Nakagawa H., Shiota S., Takano K., Shibata F., Kato H.;
RT "Cytokine-induced neutrophil chemoattractant (CINC)-2 alpha, a novel member
RT of rat GRO/CINCs, is a predominant chemokine produced by
RT lipopolysaccharide-stimulated rat macrophages in culture.";
RL Biochem. Biophys. Res. Commun. 220:945-948(1996).
CC -!- FUNCTION: Ligand for CXCR2 (By similarity). Has chemotactic activity
CC for neutrophils. May play a role in inflammation and exert its effects
CC on endothelial cells in an autocrine fashion. {ECO:0000250,
CC ECO:0000269|PubMed:8043001, ECO:0000269|PubMed:8607872}.
CC -!- SUBCELLULAR LOCATION: Secreted.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1; Synonyms=CINC-2A;
CC IsoId=Q10746-1; Sequence=Displayed;
CC Name=2; Synonyms=CINC-2B;
CC IsoId=Q10746-2; Sequence=VSP_035735;
CC -!- INDUCTION: By lipopolysaccharide. Expression increases until 4 hours
CC after treatment and then gradually decreases, remaining high 24 hours
CC after stimulation. {ECO:0000269|PubMed:9576061}.
CC -!- SIMILARITY: Belongs to the intercrine alpha (chemokine CxC) family.
CC {ECO:0000305}.
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DR EMBL; D21095; BAA04657.1; -; mRNA.
DR EMBL; D87926; BAB12336.1; -; Genomic_DNA.
DR EMBL; D87926; BAB12279.1; -; Genomic_DNA.
DR EMBL; D87927; BAB12280.1; -; mRNA.
DR PIR; S46198; S46198.
DR RefSeq; NP_612531.1; NM_138522.1. [Q10746-2]
DR RefSeq; XP_006250783.1; XM_006250721.3. [Q10746-1]
DR AlphaFoldDB; Q10746; -.
DR SMR; Q10746; -.
DR STRING; 10116.ENSRNOP00000038520; -.
DR PhosphoSitePlus; Q10746; -.
DR PaxDb; Q10746; -.
DR Ensembl; ENSRNOT00000034090; ENSRNOP00000038520; ENSRNOG00000028043. [Q10746-2]
DR Ensembl; ENSRNOT00000098120; ENSRNOP00000077076; ENSRNOG00000028043. [Q10746-1]
DR GeneID; 171551; -.
DR KEGG; rno:171551; -.
DR UCSC; RGD:621812; rat. [Q10746-1]
DR CTD; 2921; -.
DR RGD; 621812; Cxcl3.
DR eggNOG; ENOG502S7MM; Eukaryota.
DR GeneTree; ENSGT00940000155233; -.
DR HOGENOM; CLU_143902_3_0_1; -.
DR InParanoid; Q10746; -.
DR OMA; PKFITNV; -.
DR OrthoDB; 1618797at2759; -.
DR PhylomeDB; Q10746; -.
DR TreeFam; TF333433; -.
DR Reactome; R-RNO-380108; Chemokine receptors bind chemokines.
DR Reactome; R-RNO-418594; G alpha (i) signalling events.
DR Reactome; R-RNO-6798695; Neutrophil degranulation.
DR PRO; PR:Q10746; -.
DR Proteomes; UP000002494; Chromosome 14.
DR Bgee; ENSRNOG00000028043; Expressed in lung and 6 other tissues.
DR Genevisible; Q10746; RN.
DR GO; GO:0005615; C:extracellular space; IDA:RGD.
DR GO; GO:0008009; F:chemokine activity; IDA:UniProtKB.
DR GO; GO:0045236; F:CXCR chemokine receptor binding; IBA:GO_Central.
DR GO; GO:0061844; P:antimicrobial humoral immune response mediated by antimicrobial peptide; IBA:GO_Central.
DR GO; GO:0071222; P:cellular response to lipopolysaccharide; IEP:RGD.
DR GO; GO:0070098; P:chemokine-mediated signaling pathway; IBA:GO_Central.
DR GO; GO:0006954; P:inflammatory response; IMP:RGD.
DR GO; GO:0030593; P:neutrophil chemotaxis; IDA:UniProtKB.
DR GO; GO:0007204; P:positive regulation of cytosolic calcium ion concentration; IDA:RGD.
DR GO; GO:0032496; P:response to lipopolysaccharide; IEP:RGD.
DR CDD; cd00273; Chemokine_CXC; 1.
DR InterPro; IPR001089; Chemokine_CXC.
DR InterPro; IPR018048; Chemokine_CXC_CS.
DR InterPro; IPR001811; Chemokine_IL8-like_dom.
DR InterPro; IPR033899; CXC_Chemokine_domain.
DR InterPro; IPR036048; Interleukin_8-like_sf.
DR PANTHER; PTHR10179; PTHR10179; 1.
DR Pfam; PF00048; IL8; 1.
DR PRINTS; PR00437; SMALLCYTKCXC.
DR SMART; SM00199; SCY; 1.
DR SUPFAM; SSF54117; SSF54117; 1.
DR PROSITE; PS00471; SMALL_CYTOKINES_CXC; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Chemotaxis; Cytokine; Direct protein sequencing;
KW Disulfide bond; Inflammatory response; Reference proteome; Secreted;
KW Signal.
FT SIGNAL 1..32
FT /evidence="ECO:0000269|PubMed:8471066,
FT ECO:0000269|PubMed:8607872"
FT CHAIN 33..101
FT /note="C-X-C motif chemokine 3"
FT /id="PRO_0000144297"
FT DISULFID 37..63
FT /evidence="ECO:0000250"
FT DISULFID 39..79
FT /evidence="ECO:0000250"
FT VAR_SEQ 98..101
FT /note="DKSS -> PSL (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:8043001"
FT /id="VSP_035735"
SQ SEQUENCE 101 AA; 11109 MW; D949D5712FE30909 CRC64;
MAPPTRRLLN AALLLLLLLM ATSHQPSGTV VARELRCQCL KTLPRVDFEN IQSLTVTPPG
PHCTQTEVIA TLKDGQEVCL NPQAPRLQKI IQKLLKSDKS S
