CXCL5_MOUSE
ID CXCL5_MOUSE Reviewed; 132 AA.
AC P50228; Q8K3I3;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 03-OCT-2012, sequence version 2.
DT 03-AUG-2022, entry version 156.
DE RecName: Full=C-X-C motif chemokine 5;
DE AltName: Full=Cytokine LIX;
DE AltName: Full=Small-inducible cytokine B5;
DE Contains:
DE RecName: Full=GCP-2(1-78);
DE Contains:
DE RecName: Full=GCP-2(9-78);
DE Flags: Precursor;
GN Name=Cxcl5; Synonyms=Scyb5;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=7622488; DOI=10.1074/jbc.270.28.16756;
RA Smith J.B., Herschman H.R.;
RT "Glucocorticoid-attenuated response genes encode intercellular mediators,
RT including a new C-X-C chemokine.";
RL J. Biol. Chem. 270:16756-16765(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=129/Sv;
RX PubMed=12439624; DOI=10.1007/s00251-002-0501-5;
RA Smith J.B., Wadleigh D.J., Xia Y.-R., Mar R.A., Herschman H.R., Lusis A.J.;
RT "Cloning and genomic localization of the murine LPS-induced CXC chemokine
RT (LIX) gene, Scyb5.";
RL Immunogenetics 54:599-603(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=FVB/N; TISSUE=Mammary gland;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP PROTEIN SEQUENCE OF 41-118, IDENTIFICATION OF GCP-2(1-78), AND PROTEOLYTIC
RP PROCESSING OF N-TERMINUS.
RX PubMed=8759763;
RA Wuyts A., Haelens A., Proost P., Lenaerts J.-P., Conings R., Opdenakker G.,
RA Van Damme J.;
RT "Identification of mouse granulocyte chemotactic protein-2 from fibroblasts
RT and epithelial cells. Functional comparison with natural KC and macrophage
RT inflammatory protein-2.";
RL J. Immunol. 157:1736-1743(1996).
RN [6]
RP PROTEIN SEQUENCE OF 49-55, IDENTIFICATION OF GCP-2(9-78) BY MASS
RP SPECTROMETRY, FUNCTION, AND PROTEOLYTIC PROCESSING OF N-TERMINUS AND
RP C-TERMINAL.
RC TISSUE=Fibroblast;
RX PubMed=10570306;
RA Wuyts A., D'Haese A., Cremers V., Menten P., Lenaerts J.-P., De Loof A.,
RA Heremans H., Proost P., Van Damme J.;
RT "NH2- and COOH-terminal truncations of murine granulocyte chemotactic
RT protein-2 augment the in vitro and in vivo neutrophil chemotactic
RT potency.";
RL J. Immunol. 163:6155-6163(1999).
CC -!- FUNCTION: May participate in the recruitment of inflammatory cells by
CC injured or infected tissue. GCP-2(1-78) and, more potent, GCP-2(9-78)
CC attract neutrophils and are involved in neutrophil activation.
CC {ECO:0000269|PubMed:10570306}.
CC -!- SUBUNIT: Monomer. Homodimer. {ECO:0000250|UniProtKB:P42830}.
CC -!- SUBCELLULAR LOCATION: Secreted.
CC -!- INDUCTION: By lipopolysaccharide (LPS).
CC -!- PTM: GCP-2(1-78) and GCP-2(9-78) are produced by proteolytic cleavage
CC after secretion from fibroblasts and epithelial cells. GCP-2(9-78) is
CC the most prominent form. A number of additional N-terminal (processed
CC between pos. 41 and 48) and C-terminal (processed between pos. 118 and
CC 132) processed forms have been identified, probably also representing
CC intermediate states. {ECO:0000269|PubMed:10570306,
CC ECO:0000269|PubMed:8759763}.
CC -!- SIMILARITY: Belongs to the intercrine alpha (chemokine CxC) family.
CC {ECO:0000305}.
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DR EMBL; U27267; AAC52238.1; -; mRNA.
DR EMBL; AY100019; AAM48589.1; -; Genomic_DNA.
DR EMBL; AC105995; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC024392; AAH24392.1; -; mRNA.
DR CCDS; CCDS19414.1; -.
DR PIR; A57325; A57325.
DR RefSeq; NP_033167.2; NM_009141.3.
DR AlphaFoldDB; P50228; -.
DR SMR; P50228; -.
DR STRING; 10090.ENSMUSP00000031318; -.
DR iPTMnet; P50228; -.
DR PhosphoSitePlus; P50228; -.
DR PaxDb; P50228; -.
DR PRIDE; P50228; -.
DR ProteomicsDB; 279221; -.
DR ABCD; P50228; 2 sequenced antibodies.
DR Antibodypedia; 13323; 277 antibodies from 22 providers.
DR DNASU; 20311; -.
DR Ensembl; ENSMUST00000031318; ENSMUSP00000031318; ENSMUSG00000029371.
DR GeneID; 20311; -.
DR KEGG; mmu:20311; -.
DR UCSC; uc008ybg.2; mouse.
DR CTD; 6374; -.
DR MGI; MGI:1096868; Cxcl5.
DR VEuPathDB; HostDB:ENSMUSG00000029371; -.
DR eggNOG; ENOG502S7MM; Eukaryota.
DR GeneTree; ENSGT00940000162749; -.
DR HOGENOM; CLU_143902_1_0_1; -.
DR InParanoid; P50228; -.
DR OMA; KELRCTC; -.
DR OrthoDB; 1556509at2759; -.
DR PhylomeDB; P50228; -.
DR TreeFam; TF333433; -.
DR Reactome; R-MMU-380108; Chemokine receptors bind chemokines.
DR Reactome; R-MMU-418594; G alpha (i) signalling events.
DR BioGRID-ORCS; 20311; 2 hits in 75 CRISPR screens.
DR PRO; PR:P50228; -.
DR Proteomes; UP000000589; Chromosome 5.
DR RNAct; P50228; protein.
DR Bgee; ENSMUSG00000029371; Expressed in right lobe of liver and 59 other tissues.
DR Genevisible; P50228; MM.
DR GO; GO:0005615; C:extracellular space; ISO:MGI.
DR GO; GO:0008009; F:chemokine activity; ISO:MGI.
DR GO; GO:0045236; F:CXCR chemokine receptor binding; IBA:GO_Central.
DR GO; GO:0042802; F:identical protein binding; ISO:MGI.
DR GO; GO:0031100; P:animal organ regeneration; ISO:MGI.
DR GO; GO:0061844; P:antimicrobial humoral immune response mediated by antimicrobial peptide; IBA:GO_Central.
DR GO; GO:0071222; P:cellular response to lipopolysaccharide; IBA:GO_Central.
DR GO; GO:0070098; P:chemokine-mediated signaling pathway; IBA:GO_Central.
DR GO; GO:0006954; P:inflammatory response; ISO:MGI.
DR GO; GO:0001776; P:leukocyte homeostasis; IMP:MGI.
DR GO; GO:0042119; P:neutrophil activation; IEA:Ensembl.
DR GO; GO:0030593; P:neutrophil chemotaxis; IBA:GO_Central.
DR GO; GO:0010976; P:positive regulation of neuron projection development; ISO:MGI.
DR GO; GO:0046427; P:positive regulation of receptor signaling pathway via JAK-STAT; ISO:MGI.
DR GO; GO:0032642; P:regulation of chemokine production; IMP:MGI.
DR GO; GO:0070951; P:regulation of neutrophil mediated killing of gram-negative bacterium; IMP:MGI.
DR GO; GO:0032496; P:response to lipopolysaccharide; IMP:MGI.
DR CDD; cd00273; Chemokine_CXC; 1.
DR InterPro; IPR001089; Chemokine_CXC.
DR InterPro; IPR018048; Chemokine_CXC_CS.
DR InterPro; IPR001811; Chemokine_IL8-like_dom.
DR InterPro; IPR033899; CXC_Chemokine_domain.
DR InterPro; IPR036048; Interleukin_8-like_sf.
DR PANTHER; PTHR10179; PTHR10179; 1.
DR Pfam; PF00048; IL8; 1.
DR PRINTS; PR00437; SMALLCYTKCXC.
DR SMART; SM00199; SCY; 1.
DR SUPFAM; SSF54117; SSF54117; 1.
DR PROSITE; PS00471; SMALL_CYTOKINES_CXC; 1.
PE 1: Evidence at protein level;
KW Chemotaxis; Cytokine; Direct protein sequencing; Disulfide bond;
KW Inflammatory response; Reference proteome; Secreted; Signal.
FT SIGNAL 1..40
FT /evidence="ECO:0000269|PubMed:8759763"
FT CHAIN 41..132
FT /note="C-X-C motif chemokine 5"
FT /id="PRO_0000005078"
FT CHAIN 41..118
FT /note="GCP-2(1-78)"
FT /id="PRO_0000005079"
FT CHAIN 49..118
FT /note="GCP-2(9-78)"
FT /id="PRO_0000005080"
FT DISULFID 53..79
FT /evidence="ECO:0000250|UniProtKB:P42830"
FT DISULFID 55..95
FT /evidence="ECO:0000250|UniProtKB:P42830"
FT CONFLICT 10
FT /note="R -> H (in Ref. 1; AAC52238 and 4; AAH24392)"
FT /evidence="ECO:0000305"
FT CONFLICT 16
FT /note="I -> S (in Ref. 1; AAC52238 and 4; AAH24392)"
FT /evidence="ECO:0000305"
FT CONFLICT 81..82
FT /note="TV -> N (in Ref. 5; AA sequence)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 132 AA; 14235 MW; B7E9C4E6094DE365 CRC64;
MSLQLRSSAR IPSGSISPFM RMAPLAFLLL FTLPQHLAEA APSSVIAATE LRCVCLTVTP
KINPKLIANL EVIPAGPQCP TVEVIAKLKN QKEVCLDPEA PVIKKIIQKI LGSDKKKAKR
NALAVERTAS VQ
