ID   CXCL6_BOVIN             Reviewed;         112 AA.
AC   P80221; A5PJH0; Q9GKP5;
DT   01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT   04-MAY-2001, sequence version 2.
DT   03-AUG-2022, entry version 142.
DE   RecName: Full=C-X-C motif chemokine 6;
DE   AltName: Full=Granulocyte chemotactic protein 2;
DE            Short=GCP-2;
DE   AltName: Full=Small-inducible cytokine B6;
DE   Flags: Precursor;
GN   Name=CXCL6; Synonyms=GCP2, SCYB6;
OS   Bos taurus (Bovine).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC   Bovinae; Bos.
OX   NCBI_TaxID=9913;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Endometrium;
RA   Austin K.J., Perry D.J., Hansen T.R.;
RT   "The bovine granulocyte chemotactic protein-2 cDNA.";
RL   Submitted (MAY-1999) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=Hereford; TISSUE=Thymus;
RG   NIH - Mammalian Gene Collection (MGC) project;
RL   Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   PROTEIN SEQUENCE OF 37-111, FUNCTION, AND PROTEOLYTIC PROCESSING OF
RP   N-TERMINAL.
RC   TISSUE=Kidney;
RX   PubMed=8399143; DOI=10.1021/bi00089a037;
RA   Proost P., Wuyts A., Conings R., Lenaerts J.-P., Billiau A., Opdenakker G.,
RA   van Damme J.;
RT   "Human and bovine granulocyte chemotactic protein-2: complete amino acid
RT   sequence and functional characterization as chemokines.";
RL   Biochemistry 32:10170-10177(1993).
CC   -!- FUNCTION: Chemotactic for neutrophil granulocytes. Signals through
CC       binding and activation of its receptors (CXCR1 and CXCR2). In addition
CC       to its chemotactic and angiogenic properties, it has strong
CC       antibacterial activity against Gram-positive and Gram-negative bacteria
CC       (90-fold-higher when compared to CXCL5 and CXCL7) (By similarity).
CC       {ECO:0000250, ECO:0000269|PubMed:8399143}.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- DEVELOPMENTAL STAGE: Expressed in high amounts from endometria of day
CC       18-21 pregnant cows.
CC   -!- INDUCTION: By interferon tau and phorbol ester.
CC   -!- SIMILARITY: Belongs to the intercrine alpha (chemokine CxC) family.
CC       {ECO:0000305}.
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DR   EMBL; AF149249; AAG35212.1; -; mRNA.
DR   EMBL; BC142108; AAI42109.1; -; mRNA.
DR   PIR; B54188; B54188.
DR   RefSeq; NP_776725.1; NM_174300.2.
DR   AlphaFoldDB; P80221; -.
DR   SMR; P80221; -.
DR   STRING; 9913.ENSBTAP00000012939; -.
DR   PaxDb; P80221; -.
DR   PRIDE; P80221; -.
DR   Ensembl; ENSBTAT00000012939; ENSBTAP00000012939; ENSBTAG00000009812.
DR   GeneID; 281735; -.
DR   KEGG; bta:281735; -.
DR   CTD; 6374; -.
DR   VEuPathDB; HostDB:ENSBTAG00000009812; -.
DR   eggNOG; ENOG502S7MM; Eukaryota.
DR   GeneTree; ENSGT00940000162749; -.
DR   HOGENOM; CLU_143902_1_0_1; -.
DR   InParanoid; P80221; -.
DR   OMA; CVCLQTT; -.
DR   OrthoDB; 1556509at2759; -.
DR   TreeFam; TF333433; -.
DR   Proteomes; UP000009136; Chromosome 6.
DR   Bgee; ENSBTAG00000009812; Expressed in milk and 87 other tissues.
DR   GO; GO:0005615; C:extracellular space; IBA:GO_Central.
DR   GO; GO:0008009; F:chemokine activity; IDA:UniProtKB.
DR   GO; GO:0045236; F:CXCR chemokine receptor binding; IBA:GO_Central.
DR   GO; GO:0008201; F:heparin binding; IEA:UniProtKB-KW.
DR   GO; GO:0061844; P:antimicrobial humoral immune response mediated by antimicrobial peptide; IDA:UniProtKB.
DR   GO; GO:0071222; P:cellular response to lipopolysaccharide; IDA:UniProtKB.
DR   GO; GO:0070098; P:chemokine-mediated signaling pathway; IBA:GO_Central.
DR   GO; GO:0042742; P:defense response to bacterium; IEA:UniProtKB-KW.
DR   GO; GO:0006954; P:inflammatory response; IBA:GO_Central.
DR   GO; GO:0042119; P:neutrophil activation; IDA:UniProtKB.
DR   GO; GO:0030593; P:neutrophil chemotaxis; IDA:UniProtKB.
DR   CDD; cd00273; Chemokine_CXC; 1.
DR   InterPro; IPR001089; Chemokine_CXC.
DR   InterPro; IPR018048; Chemokine_CXC_CS.
DR   InterPro; IPR001811; Chemokine_IL8-like_dom.
DR   InterPro; IPR033899; CXC_Chemokine_domain.
DR   InterPro; IPR036048; Interleukin_8-like_sf.
DR   PANTHER; PTHR10179; PTHR10179; 1.
DR   Pfam; PF00048; IL8; 1.
DR   PRINTS; PR00437; SMALLCYTKCXC.
DR   SMART; SM00199; SCY; 1.
DR   SUPFAM; SSF54117; SSF54117; 1.
DR   PROSITE; PS00471; SMALL_CYTOKINES_CXC; 1.
PE   1: Evidence at protein level;
KW   Antibiotic; Antimicrobial; Chemotaxis; Cytokine; Direct protein sequencing;
KW   Disulfide bond; Heparin-binding; Reference proteome; Secreted; Signal.
FT   SIGNAL          1..36
FT                   /evidence="ECO:0000269|PubMed:8399143"
FT   CHAIN           37..112
FT                   /note="C-X-C motif chemokine 6"
FT                   /id="PRO_0000005082"
FT   DISULFID        48..74
FT                   /evidence="ECO:0000250"
FT   DISULFID        50..90
FT                   /evidence="ECO:0000250"
FT   VARIANT         37..45
FT                   /note="Missing (in N-terminal processing variant)"
FT   VARIANT         37..44
FT                   /note="Missing (in N-terminal processing variant)"
FT   VARIANT         37..43
FT                   /note="Missing (in N-terminal processing variant)"
FT   VARIANT         37..38
FT                   /note="Missing (in N-terminal processing variant)"
SQ   SEQUENCE   112 AA;  11589 MW;  6ED8148B96A7C082 CRC64;
     MRLLSSRAAR VSGPSGSLCA LLALLLLTPP GPLASAGPVA AVVRELRCVC LTTTPGIHPK
     TVSDLQVIAA GPQCSKVEVI ATLKNGREVC LDPEAPLIKK IVQKILDSGK NN