CXE11_ARATH
ID CXE11_ARATH Reviewed; 460 AA.
AC Q9LK21; Q6NKX4;
DT 11-JAN-2011, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 126.
DE RecName: Full=Probable carboxylesterase 11;
DE AltName: Full=AtCXE11;
DE EC=3.1.1.1;
GN Name=CXE11; OrderedLocusNames=At3g27320; ORFNames=K17E12.14;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10907853; DOI=10.1093/dnares/7.3.217;
RA Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence
RT features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC
RT clones.";
RL DNA Res. 7:217-221(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=cv. Columbia;
RA Shinn P., Chen H., Cheuk R.F., Kim C.J., Carninci P., Hayashizaki Y.,
RA Ishida J., Kamiya A., Kawai J., Narusaka M., Sakurai T., Satou M., Seki M.,
RA Shinozaki K., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (DEC-2004) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RX PubMed=14993207; DOI=10.1101/gr.1515604;
RA Castelli V., Aury J.-M., Jaillon O., Wincker P., Clepet C., Menard M.,
RA Cruaud C., Quetier F., Scarpelli C., Schaechter V., Temple G., Caboche M.,
RA Weissenbach J., Salanoubat M.;
RT "Whole genome sequence comparisons and 'full-length' cDNA sequences: a
RT combined approach to evaluate and improve Arabidopsis genome annotation.";
RL Genome Res. 14:406-413(2004).
RN [6]
RP TISSUE SPECIFICITY, GENE FAMILY, AND NOMENCLATURE.
RX PubMed=14738307; DOI=10.1007/s00239-003-2492-8;
RA Marshall S.D., Putterill J.J., Plummer K.M., Newcomb R.D.;
RT "The carboxylesterase gene family from Arabidopsis thaliana.";
RL J. Mol. Evol. 57:487-500(2003).
CC -!- FUNCTION: Carboxylesterase acting on esters with varying acyl chain
CC length. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a carboxylic ester + H2O = a carboxylate + an alcohol + H(+);
CC Xref=Rhea:RHEA:21164, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:29067, ChEBI:CHEBI:30879, ChEBI:CHEBI:33308; EC=3.1.1.1;
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q9LK21-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9LK21-2; Sequence=VSP_040307;
CC -!- TISSUE SPECIFICITY: Expressed in roots, leaves, stems, flowers and
CC siliques. {ECO:0000269|PubMed:14738307}.
CC -!- SIMILARITY: Belongs to the 'GDXG' lipolytic enzyme family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BX824353; Type=Miscellaneous discrepancy; Note=Sequencing errors.; Evidence={ECO:0000305};
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DR EMBL; AP000381; BAB02127.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE77294.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE77295.1; -; Genomic_DNA.
DR EMBL; BT012569; AAS99713.1; -; mRNA.
DR EMBL; AK176881; BAD44644.1; -; mRNA.
DR EMBL; BX824353; -; NOT_ANNOTATED_CDS; mRNA.
DR RefSeq; NP_001030781.1; NM_001035704.2. [Q9LK21-2]
DR RefSeq; NP_189367.1; NM_113646.4. [Q9LK21-1]
DR AlphaFoldDB; Q9LK21; -.
DR SMR; Q9LK21; -.
DR STRING; 3702.AT3G27320.1; -.
DR ESTHER; arath-CXE11; Plant_carboxylesterase.
DR MEROPS; S09.A05; -.
DR iPTMnet; Q9LK21; -.
DR PaxDb; Q9LK21; -.
DR PRIDE; Q9LK21; -.
DR ProteomicsDB; 220511; -. [Q9LK21-1]
DR EnsemblPlants; AT3G27320.1; AT3G27320.1; AT3G27320. [Q9LK21-1]
DR EnsemblPlants; AT3G27320.2; AT3G27320.2; AT3G27320. [Q9LK21-2]
DR GeneID; 822351; -.
DR Gramene; AT3G27320.1; AT3G27320.1; AT3G27320. [Q9LK21-1]
DR Gramene; AT3G27320.2; AT3G27320.2; AT3G27320. [Q9LK21-2]
DR KEGG; ath:AT3G27320; -.
DR Araport; AT3G27320; -.
DR TAIR; locus:2086503; AT3G27320.
DR eggNOG; KOG1515; Eukaryota.
DR InParanoid; Q9LK21; -.
DR OMA; SMCILAW; -.
DR PhylomeDB; Q9LK21; -.
DR PRO; PR:Q9LK21; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q9LK21; baseline and differential.
DR Genevisible; Q9LK21; AT.
DR GO; GO:0080030; F:methyl indole-3-acetate esterase activity; IEA:UniProtKB-EC.
DR Gene3D; 3.40.50.1820; -; 1.
DR InterPro; IPR029058; AB_hydrolase.
DR InterPro; IPR013094; AB_hydrolase_3.
DR Pfam; PF07859; Abhydrolase_3; 1.
DR SUPFAM; SSF53474; SSF53474; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Hydrolase; Reference proteome; Serine esterase.
FT CHAIN 1..460
FT /note="Probable carboxylesterase 11"
FT /id="PRO_0000402556"
FT REGION 26..52
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 132..161
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 173..175
FT /note="Involved in the stabilization of the negatively
FT charged intermediate by the formation of the oxyanion hole"
FT /evidence="ECO:0000250|UniProtKB:Q5NUF3"
FT COMPBIAS 26..40
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 289
FT /evidence="ECO:0000250|UniProtKB:Q5NUF3"
FT ACT_SITE 392
FT /evidence="ECO:0000250|UniProtKB:Q5NUF3"
FT ACT_SITE 422
FT /evidence="ECO:0000250|UniProtKB:Q5NUF3"
FT VAR_SEQ 96..127
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|Ref.3, ECO:0000303|Ref.4"
FT /id="VSP_040307"
FT CONFLICT 218
FT /note="A -> G (in Ref. 3; AAS99713 and 4; BAD44644)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 460 AA; 50531 MW; F6E0637960621478 CRC64;
MPSVGVKLYS VFFKFLLKHR LQNRIQSSGD ESSSDPFGVT TRPEESVAAP NPLFTDGVAT
KDIHIDPLTS LSVRIFLPES ALTPLEPSTS ACVYSGKART LNNIAGSDLL SRRNSLGSSN
SLLSHKVESR RNSYGYTTGS SSPEAGSSDV YRGYAPSSSG GNSRKLPVML QFHGGGWVSG
SNDSVANDFF CRRMAKHCDI IVLAVGYRLA PENRYPAACE DGFKVLKWLG KQANLAECNK
SMGNSRRPGG EVKKSEVNKH IVDAFGASLV EPWLANHADP SRCVLLGVSC GANIADYVAR
KAIEVGQNLD PVKVVAQVLM YPFFIGSVPT QSEIKQANSY FYDKPMCILA WKLFLPEEEF
SLDHQAANPL VPGRSPPLKF MPPTLTIVAE HDWMRDRAIA YSEELRKVNV DAPVLEYKDA
VHEFATLDML LRTPQAQACA EDIAIWAKKY ISLRGHEFSY
