CXE15_ARATH
ID CXE15_ARATH Reviewed; 329 AA.
AC Q9FG13;
DT 11-JAN-2011, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 121.
DE RecName: Full=Probable carboxylesterase 15;
DE AltName: Full=AtCXE15;
DE EC=3.1.1.1;
GN Name=CXE15; OrderedLocusNames=At5g06570; ORFNames=F15M7.10;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RA Kaneko T., Katoh T., Asamizu E., Sato S., Nakamura Y., Kotani H.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. XI.";
RL Submitted (JUL-2000) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=11910074; DOI=10.1126/science.1071006;
RA Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T.,
RA Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y.,
RA Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K.,
RA Shinagawa A., Shinozaki K.;
RT "Functional annotation of a full-length Arabidopsis cDNA collection.";
RL Science 296:141-145(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP TISSUE SPECIFICITY, GENE FAMILY, AND NOMENCLATURE.
RX PubMed=14738307; DOI=10.1007/s00239-003-2492-8;
RA Marshall S.D., Putterill J.J., Plummer K.M., Newcomb R.D.;
RT "The carboxylesterase gene family from Arabidopsis thaliana.";
RL J. Mol. Evol. 57:487-500(2003).
CC -!- FUNCTION: Carboxylesterase acting on esters with varying acyl chain
CC length. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a carboxylic ester + H2O = a carboxylate + an alcohol + H(+);
CC Xref=Rhea:RHEA:21164, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:29067, ChEBI:CHEBI:30879, ChEBI:CHEBI:33308; EC=3.1.1.1;
CC -!- TISSUE SPECIFICITY: Expressed in roots, leaves, stems, flowers and
CC siliques. {ECO:0000269|PubMed:14738307}.
CC -!- SIMILARITY: Belongs to the 'GDXG' lipolytic enzyme family.
CC {ECO:0000305}.
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DR EMBL; AP002543; BAB11406.1; -; Genomic_DNA.
DR EMBL; CP002688; AED91035.1; -; Genomic_DNA.
DR EMBL; CP002688; AED91036.1; -; Genomic_DNA.
DR EMBL; AK118580; BAC43180.1; -; mRNA.
DR EMBL; BT008350; AAP37709.1; -; mRNA.
DR RefSeq; NP_196275.1; NM_120740.3.
DR RefSeq; NP_850782.1; NM_180451.2.
DR AlphaFoldDB; Q9FG13; -.
DR SMR; Q9FG13; -.
DR BioGRID; 15824; 1.
DR IntAct; Q9FG13; 1.
DR STRING; 3702.AT5G06570.2; -.
DR ESTHER; arath-CXE15; Plant_carboxylesterase.
DR PaxDb; Q9FG13; -.
DR PRIDE; Q9FG13; -.
DR ProteomicsDB; 220433; -.
DR EnsemblPlants; AT5G06570.1; AT5G06570.1; AT5G06570.
DR EnsemblPlants; AT5G06570.2; AT5G06570.2; AT5G06570.
DR GeneID; 830545; -.
DR Gramene; AT5G06570.1; AT5G06570.1; AT5G06570.
DR Gramene; AT5G06570.2; AT5G06570.2; AT5G06570.
DR KEGG; ath:AT5G06570; -.
DR Araport; AT5G06570; -.
DR TAIR; locus:2144083; AT5G06570.
DR eggNOG; KOG1515; Eukaryota.
DR HOGENOM; CLU_012494_22_1_1; -.
DR InParanoid; Q9FG13; -.
DR OMA; KLWEHGV; -.
DR PhylomeDB; Q9FG13; -.
DR PRO; PR:Q9FG13; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9FG13; baseline and differential.
DR Genevisible; Q9FG13; AT.
DR GO; GO:0080030; F:methyl indole-3-acetate esterase activity; IEA:UniProtKB-EC.
DR Gene3D; 3.40.50.1820; -; 1.
DR InterPro; IPR029058; AB_hydrolase.
DR InterPro; IPR013094; AB_hydrolase_3.
DR Pfam; PF07859; Abhydrolase_3; 1.
DR SUPFAM; SSF53474; SSF53474; 1.
PE 2: Evidence at transcript level;
KW Hydrolase; Reference proteome; Serine esterase.
FT CHAIN 1..329
FT /note="Probable carboxylesterase 15"
FT /id="PRO_0000402559"
FT MOTIF 83..85
FT /note="Involved in the stabilization of the negatively
FT charged intermediate by the formation of the oxyanion hole"
FT /evidence="ECO:0000250|UniProtKB:Q5NUF3"
FT ACT_SITE 169
FT /evidence="ECO:0000250|UniProtKB:Q5NUF3"
FT ACT_SITE 271
FT /evidence="ECO:0000250|UniProtKB:Q5NUF3"
FT ACT_SITE 302
FT /evidence="ECO:0000250|UniProtKB:Q5NUF3"
SQ SEQUENCE 329 AA; 36597 MW; 000140D8BB91126A CRC64;
MGSLGEEPQV AEDCMGLLQL LSNGTVLRSE SIDLITQQIP FKNNQTVLFK DSIYHKPNNL
HLRLYKPISA SNRTALPVVV FFHGGGFCFG SRSWPHFHNF CLTLASSLNA LVVSPDYRLA
PEHRLPAAFE DAEAVLTWLW DQAVSDGVNH WFEDGTDVDF DRVFVVGDSS GGNIAHQLAV
RFGSGSIELT PVRVRGYVLM GPFFGGEERT NSENGPSEAL LSLDLLDKFW RLSLPNGATR
DHHMANPFGP TSPTLESISL EPMLVIVGGS ELLRDRAKEY AYKLKKMGGK RVDYIEFENK
EHGFYSNYPS SEAAEQVLRI IGDFMNNLS
