ID   CXE20_ARATH             Reviewed;         327 AA.
AC   Q9LVB8;
DT   11-JAN-2011, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2000, sequence version 1.
DT   03-AUG-2022, entry version 107.
DE   RecName: Full=Probable carboxylesterase 120;
DE   AltName: Full=AtCXE20;
DE            EC=3.1.1.1;
GN   Name=CXE20; OrderedLocusNames=At5g62180; ORFNames=MMI9.26;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=10718197; DOI=10.1093/dnares/7.1.31;
RA   Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Kotani H.,
RA   Tabata S.;
RT   "Structural analysis of Arabidopsis thaliana chromosome 5. X. Sequence
RT   features of the regions of 3,076,755 bp covered by sixty P1 and TAC
RT   clones.";
RL   DNA Res. 7:31-63(2000).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   TISSUE SPECIFICITY, GENE FAMILY, AND NOMENCLATURE.
RX   PubMed=14738307; DOI=10.1007/s00239-003-2492-8;
RA   Marshall S.D., Putterill J.J., Plummer K.M., Newcomb R.D.;
RT   "The carboxylesterase gene family from Arabidopsis thaliana.";
RL   J. Mol. Evol. 57:487-500(2003).
CC   -!- FUNCTION: Carboxylesterase acting on esters with varying acyl chain
CC       length. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a carboxylic ester + H2O = a carboxylate + an alcohol + H(+);
CC         Xref=Rhea:RHEA:21164, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:29067, ChEBI:CHEBI:30879, ChEBI:CHEBI:33308; EC=3.1.1.1;
CC   -!- TISSUE SPECIFICITY: Expressed in roots, stems, flowers and siliques.
CC       {ECO:0000269|PubMed:14738307}.
CC   -!- SIMILARITY: Belongs to the 'GDXG' lipolytic enzyme family.
CC       {ECO:0000305}.
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DR   EMBL; AB019235; BAA97182.1; -; Genomic_DNA.
DR   EMBL; CP002688; AED97578.1; -; Genomic_DNA.
DR   RefSeq; NP_201024.1; NM_125612.3.
DR   AlphaFoldDB; Q9LVB8; -.
DR   SMR; Q9LVB8; -.
DR   STRING; 3702.AT5G62180.1; -.
DR   ESTHER; arath-AT5G62180; Plant_carboxylesterase.
DR   MEROPS; S09.A14; -.
DR   PaxDb; Q9LVB8; -.
DR   PRIDE; Q9LVB8; -.
DR   ProteomicsDB; 220514; -.
DR   EnsemblPlants; AT5G62180.1; AT5G62180.1; AT5G62180.
DR   GeneID; 836339; -.
DR   Gramene; AT5G62180.1; AT5G62180.1; AT5G62180.
DR   KEGG; ath:AT5G62180; -.
DR   Araport; AT5G62180; -.
DR   TAIR; locus:2174033; AT5G62180.
DR   eggNOG; KOG1515; Eukaryota.
DR   HOGENOM; CLU_012494_22_1_1; -.
DR   InParanoid; Q9LVB8; -.
DR   OMA; SHFRMGD; -.
DR   OrthoDB; 1263520at2759; -.
DR   PhylomeDB; Q9LVB8; -.
DR   BioCyc; ARA:AT5G62180-MON; -.
DR   PRO; PR:Q9LVB8; -.
DR   Proteomes; UP000006548; Chromosome 5.
DR   ExpressionAtlas; Q9LVB8; baseline and differential.
DR   Genevisible; Q9LVB8; AT.
DR   GO; GO:0043722; F:4-hydroxyphenylacetate decarboxylase activity; IDA:TAIR.
DR   GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.50.1820; -; 1.
DR   InterPro; IPR029058; AB_hydrolase.
DR   InterPro; IPR013094; AB_hydrolase_3.
DR   Pfam; PF07859; Abhydrolase_3; 1.
DR   SUPFAM; SSF53474; SSF53474; 1.
PE   2: Evidence at transcript level;
KW   Hydrolase; Reference proteome; Serine esterase.
FT   CHAIN           1..327
FT                   /note="Probable carboxylesterase 120"
FT                   /id="PRO_0000402563"
FT   MOTIF           87..89
FT                   /note="Involved in the stabilization of the negatively
FT                   charged intermediate by the formation of the oxyanion hole"
FT                   /evidence="ECO:0000250|UniProtKB:Q5NUF3"
FT   ACT_SITE        166
FT                   /evidence="ECO:0000250|UniProtKB:Q0ZPV7"
FT   ACT_SITE        272
FT                   /evidence="ECO:0000250|UniProtKB:Q0ZPV7"
FT   ACT_SITE        302
FT                   /evidence="ECO:0000250|UniProtKB:Q0ZPV7"
SQ   SEQUENCE   327 AA;  36182 MW;  245C7253A92366F6 CRC64;
     MSEPSPIADP YAYLNIVNNP DGSITRDLSN FPCTAATPDP SPLNPAVSKD LPVNQLKSTW
     LRLYLPSSAV NEGNVSSQKL PIVVYYHGGG FILCSVDMQL FHDFCSEVAR DLNAIVVSPS
     YRLAPEHRLP AAYDDGVEAL DWIKTSDDEW IKSHADFSNV FLMGTSAGGN LAYNVGLRSV
     DSVSDLSPLQ IRGLILHHPF FGGEERSESE IRLMNDQVCP PIVTDVMWDL SLPVGVDRDH
     EYSNPTVGDG SEKLEKIGRL RWKVMMIGGE DDPMIDLQKD VAKLMKKKGV EVVEHYTGGH
     VHGAEIRDPS KRKTLFLSIK NFIFSVL