CXE2_ARATH
ID CXE2_ARATH Reviewed; 314 AA.
AC Q9SX78; Q8LG06;
DT 11-JAN-2011, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 121.
DE RecName: Full=Probable carboxylesterase 2;
DE AltName: Full=AtCXE2;
DE EC=3.1.1.1;
GN Name=CXE2; OrderedLocusNames=At1g47480; ORFNames=F16N3.25;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP TISSUE SPECIFICITY, GENE FAMILY, AND NOMENCLATURE.
RX PubMed=14738307; DOI=10.1007/s00239-003-2492-8;
RA Marshall S.D., Putterill J.J., Plummer K.M., Newcomb R.D.;
RT "The carboxylesterase gene family from Arabidopsis thaliana.";
RL J. Mol. Evol. 57:487-500(2003).
CC -!- FUNCTION: Carboxylesterase acting on esters with varying acyl chain
CC length. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a carboxylic ester + H2O = a carboxylate + an alcohol + H(+);
CC Xref=Rhea:RHEA:21164, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:29067, ChEBI:CHEBI:30879, ChEBI:CHEBI:33308; EC=3.1.1.1;
CC -!- TISSUE SPECIFICITY: Expressed in roots and flowers.
CC {ECO:0000269|PubMed:14738307}.
CC -!- SIMILARITY: Belongs to the 'GDXG' lipolytic enzyme family.
CC {ECO:0000305}.
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DR EMBL; AC007519; AAD46039.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE32171.1; -; Genomic_DNA.
DR EMBL; AY084535; AAM61103.1; -; mRNA.
DR PIR; A96515; A96515.
DR RefSeq; NP_564507.1; NM_103640.3.
DR AlphaFoldDB; Q9SX78; -.
DR SMR; Q9SX78; -.
DR STRING; 3702.AT1G47480.1; -.
DR ESTHER; arath-F16N3.25; Plant_carboxylesterase.
DR PaxDb; Q9SX78; -.
DR PRIDE; Q9SX78; -.
DR ProteomicsDB; 220377; -.
DR EnsemblPlants; AT1G47480.1; AT1G47480.1; AT1G47480.
DR GeneID; 841155; -.
DR Gramene; AT1G47480.1; AT1G47480.1; AT1G47480.
DR KEGG; ath:AT1G47480; -.
DR Araport; AT1G47480; -.
DR TAIR; locus:2015413; AT1G47480.
DR eggNOG; KOG1515; Eukaryota.
DR HOGENOM; CLU_012494_22_0_1; -.
DR InParanoid; Q9SX78; -.
DR OMA; NISHFMA; -.
DR OrthoDB; 1263520at2759; -.
DR PhylomeDB; Q9SX78; -.
DR BioCyc; ARA:AT1G47480-MON; -.
DR PRO; PR:Q9SX78; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9SX78; baseline and differential.
DR Genevisible; Q9SX78; AT.
DR GO; GO:0080030; F:methyl indole-3-acetate esterase activity; IEA:UniProtKB-EC.
DR Gene3D; 3.40.50.1820; -; 1.
DR InterPro; IPR029058; AB_hydrolase.
DR InterPro; IPR013094; AB_hydrolase_3.
DR InterPro; IPR002168; Lipase_GDXG_HIS_AS.
DR Pfam; PF07859; Abhydrolase_3; 1.
DR SUPFAM; SSF53474; SSF53474; 1.
DR PROSITE; PS01173; LIPASE_GDXG_HIS; 1.
PE 2: Evidence at transcript level;
KW Hydrolase; Reference proteome; Serine esterase.
FT CHAIN 1..314
FT /note="Probable carboxylesterase 2"
FT /id="PRO_0000402548"
FT MOTIF 79..81
FT /note="Involved in the stabilization of the negatively
FT charged intermediate by the formation of the oxyanion hole"
FT /evidence="ECO:0000250|UniProtKB:Q5NUF3"
FT ACT_SITE 158
FT /evidence="ECO:0000250|UniProtKB:Q5NUF3"
FT ACT_SITE 254
FT /evidence="ECO:0000250|UniProtKB:Q5NUF3"
FT ACT_SITE 286
FT /evidence="ECO:0000250|UniProtKB:Q5NUF3"
FT CONFLICT 133..134
FT /note="KN -> NT (in Ref. 3; AAM61103)"
FT /evidence="ECO:0000305"
FT CONFLICT 152
FT /note="L -> I (in Ref. 3; AAM61103)"
FT /evidence="ECO:0000305"
FT CONFLICT 178
FT /note="L -> V (in Ref. 3; AAM61103)"
FT /evidence="ECO:0000305"
FT CONFLICT 205
FT /note="R -> M (in Ref. 3; AAM61103)"
FT /evidence="ECO:0000305"
FT CONFLICT 220
FT /note="E -> K (in Ref. 3; AAM61103)"
FT /evidence="ECO:0000305"
FT CONFLICT 264
FT /note="Y -> F (in Ref. 3; AAM61103)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 314 AA; 35255 MW; F650CA0F5D2A4BA8 CRC64;
MESTKKQVSL ELLPWLVVHT DGTVERLAGT EVCPPGLDPI TGVFSKDIII EPKTGLSARI
YRPFSIQPGQ KIPLMLYFHG GAFLISSTSF PSYHTSLNKI VNQANVIAVS VNYRLAPEHP
LPTAYEDSWT ALKNIQAINE PWINDYADLD SLFLVGDSAG ANISHHLAFR AKQSDQTLKI
KGIGMIHPYF WGTQPIGAEI KDEARKQMVD GWWEFVCPSE KGSDDPWINP FADGSPDLGG
LGCERVMITV AEKDILNERG KMYYERLVKS EWKGKVEIME TKEKDHVFHI FEPDCDEAME
MVRCLALFIN QVEA
