ID   CXE3_ARATH              Reviewed;         315 AA.
AC   Q9FX92; Q6E298;
DT   11-JAN-2011, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   03-AUG-2022, entry version 113.
DE   RecName: Full=Probable carboxylesterase 3;
DE   AltName: Full=AtCXE3;
DE            EC=3.1.1.1;
GN   Name=CXE3; OrderedLocusNames=At1g49640; ORFNames=F14J22.12;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=11130712; DOI=10.1038/35048500;
RA   Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA   Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA   Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA   Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA   Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA   Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA   Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA   Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA   Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA   Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA   Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA   Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA   Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA   Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA   Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT   "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL   Nature 408:816-820(2000).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RA   Xiao Y.-L., Underwood B.A., Moskal W.A. Jr., Torian U., Redman J.C.,
RA   Wu H.C., Utterback T., Town C.D.;
RT   "Reconstruction of cDNA sequences for hypothetical genes in Arabidopsis
RT   thaliana from 5' and 3' RACE products.";
RL   Submitted (APR-2004) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RA   Underwood B.A., Xiao Y.-L., Moskal W.A. Jr., Monaghan E.L., Wang W.,
RA   Redman J.C., Wu H.C., Utterback T., Town C.D.;
RL   Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
RN   [5]
RP   TISSUE SPECIFICITY, GENE FAMILY, AND NOMENCLATURE.
RX   PubMed=14738307; DOI=10.1007/s00239-003-2492-8;
RA   Marshall S.D., Putterill J.J., Plummer K.M., Newcomb R.D.;
RT   "The carboxylesterase gene family from Arabidopsis thaliana.";
RL   J. Mol. Evol. 57:487-500(2003).
CC   -!- FUNCTION: Carboxylesterase acting on esters with varying acyl chain
CC       length. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a carboxylic ester + H2O = a carboxylate + an alcohol + H(+);
CC         Xref=Rhea:RHEA:21164, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:29067, ChEBI:CHEBI:30879, ChEBI:CHEBI:33308; EC=3.1.1.1;
CC   -!- TISSUE SPECIFICITY: Expressed in flowers and siliques.
CC       {ECO:0000269|PubMed:14738307}.
CC   -!- SIMILARITY: Belongs to the 'GDXG' lipolytic enzyme family.
CC       {ECO:0000305}.
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DR   EMBL; AC011807; AAG13050.1; -; Genomic_DNA.
DR   EMBL; CP002684; AEE32454.1; -; Genomic_DNA.
DR   EMBL; AY600525; AAT68324.1; -; mRNA.
DR   EMBL; AY649310; AAT69227.1; -; mRNA.
DR   PIR; B96533; B96533.
DR   RefSeq; NP_175387.1; NM_103852.3.
DR   AlphaFoldDB; Q9FX92; -.
DR   SMR; Q9FX92; -.
DR   STRING; 3702.AT1G49640.1; -.
DR   ESTHER; arath-F14J22.12; Plant_carboxylesterase.
DR   PaxDb; Q9FX92; -.
DR   PRIDE; Q9FX92; -.
DR   ProteomicsDB; 220378; -.
DR   EnsemblPlants; AT1G49640.1; AT1G49640.1; AT1G49640.
DR   GeneID; 841388; -.
DR   Gramene; AT1G49640.1; AT1G49640.1; AT1G49640.
DR   KEGG; ath:AT1G49640; -.
DR   Araport; AT1G49640; -.
DR   TAIR; locus:2012196; AT1G49640.
DR   eggNOG; KOG1515; Eukaryota.
DR   HOGENOM; CLU_012494_22_0_1; -.
DR   InParanoid; Q9FX92; -.
DR   OMA; SDDWINE; -.
DR   OrthoDB; 1263520at2759; -.
DR   PhylomeDB; Q9FX92; -.
DR   BioCyc; ARA:AT1G49640-MON; -.
DR   PRO; PR:Q9FX92; -.
DR   Proteomes; UP000006548; Chromosome 1.
DR   ExpressionAtlas; Q9FX92; baseline and differential.
DR   GO; GO:0016787; F:hydrolase activity; IBA:GO_Central.
DR   GO; GO:0080030; F:methyl indole-3-acetate esterase activity; IEA:UniProtKB-EC.
DR   Gene3D; 3.40.50.1820; -; 1.
DR   InterPro; IPR029058; AB_hydrolase.
DR   InterPro; IPR013094; AB_hydrolase_3.
DR   Pfam; PF07859; Abhydrolase_3; 1.
DR   SUPFAM; SSF53474; SSF53474; 1.
PE   2: Evidence at transcript level;
KW   Acetylation; Hydrolase; Reference proteome; Serine esterase.
FT   CHAIN           1..315
FT                   /note="Probable carboxylesterase 3"
FT                   /id="PRO_0000402549"
FT   MOTIF           81..83
FT                   /note="Involved in the stabilization of the negatively
FT                   charged intermediate by the formation of the oxyanion hole"
FT                   /evidence="ECO:0000250|UniProtKB:Q5NUF3"
FT   ACT_SITE        160
FT                   /evidence="ECO:0000250|UniProtKB:Q5NUF3"
FT   ACT_SITE        258
FT                   /evidence="ECO:0000250|UniProtKB:Q5NUF3"
FT   ACT_SITE        290
FT                   /evidence="ECO:0000250|UniProtKB:Q5NUF3"
FT   MOD_RES         1
FT                   /note="N-acetylmethionine"
FT                   /evidence="ECO:0000250|UniProtKB:Q9SMN0"
SQ   SEQUENCE   315 AA;  35429 MW;  4E5C7DE96D9F31B0 CRC64;
     MESDLTTEHH LPFIRIHKNG RVERLSGNDI KPTSLNPQND VVSKDVMYSS DHNLSVRMFL
     PNKSRKLDTA GNKIPLLIYF HGGAYIIQSP FSPVYHNYLT EVVITANCLA VSVQYRLAPE
     HPVPAAYDDS WSAIQWIFSH SDDWINEYAD FDRVFIAGDS AGANISHHMG IRAGKEKLSP
     TIKGIVMVHP GFWGKEPIDE HDVQDGEVRN KIAYIWENIV SPNSVDGVND PWFNVVGSGS
     DVSEMGCEKV LVAVAGKDVF WRQGLAYAAK LEKSQWKGSV EVIEEEEEGH CFHLHNHNSQ
     NASKLMQKFL EFIIS