CXE7_ARATH
ID CXE7_ARATH Reviewed; 312 AA.
AC Q9ZQ91; Q6DBI8; Q84WE2;
DT 11-JAN-2011, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1999, sequence version 1.
DT 03-AUG-2022, entry version 119.
DE RecName: Full=Probable carboxylesterase 7;
DE AltName: Full=AtCXE7;
DE EC=3.1.1.1;
GN Name=CXE7; OrderedLocusNames=At2g03550; ORFNames=T4M8.1;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 5-312.
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 12-312.
RC STRAIN=cv. Columbia;
RA Kim C.J., Chen H., Cheuk R.F., Shinn P., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (JUL-2004) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP TISSUE SPECIFICITY, GENE FAMILY, AND NOMENCLATURE.
RX PubMed=14738307; DOI=10.1007/s00239-003-2492-8;
RA Marshall S.D., Putterill J.J., Plummer K.M., Newcomb R.D.;
RT "The carboxylesterase gene family from Arabidopsis thaliana.";
RL J. Mol. Evol. 57:487-500(2003).
CC -!- FUNCTION: Carboxylesterase acting on esters with varying acyl chain
CC length. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a carboxylic ester + H2O = a carboxylate + an alcohol + H(+);
CC Xref=Rhea:RHEA:21164, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:29067, ChEBI:CHEBI:30879, ChEBI:CHEBI:33308; EC=3.1.1.1;
CC -!- TISSUE SPECIFICITY: Expressed in leaves, stems, flowers and siliques.
CC {ECO:0000269|PubMed:14738307}.
CC -!- SIMILARITY: Belongs to the 'GDXG' lipolytic enzyme family.
CC {ECO:0000305}.
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DR EMBL; AC006284; AAD17422.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC05713.1; -; Genomic_DNA.
DR EMBL; BT003917; AAO41964.1; -; mRNA.
DR EMBL; BT015034; AAT70485.1; -; mRNA.
DR PIR; G84449; G84449.
DR RefSeq; NP_178453.1; NM_126405.2.
DR AlphaFoldDB; Q9ZQ91; -.
DR SMR; Q9ZQ91; -.
DR STRING; 3702.AT2G03550.1; -.
DR ChEMBL; CHEMBL1932909; -.
DR ESTHER; arath-AT2G03550; Plant_carboxylesterase.
DR iPTMnet; Q9ZQ91; -.
DR PaxDb; Q9ZQ91; -.
DR PRIDE; Q9ZQ91; -.
DR ProteomicsDB; 220328; -.
DR EnsemblPlants; AT2G03550.1; AT2G03550.1; AT2G03550.
DR GeneID; 814884; -.
DR Gramene; AT2G03550.1; AT2G03550.1; AT2G03550.
DR KEGG; ath:AT2G03550; -.
DR Araport; AT2G03550; -.
DR TAIR; locus:2063751; AT2G03550.
DR eggNOG; KOG1515; Eukaryota.
DR HOGENOM; CLU_012494_22_0_1; -.
DR InParanoid; Q9ZQ91; -.
DR OMA; QWINKHA; -.
DR OrthoDB; 1263520at2759; -.
DR PhylomeDB; Q9ZQ91; -.
DR BioCyc; ARA:AT2G03550-MON; -.
DR PRO; PR:Q9ZQ91; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q9ZQ91; baseline and differential.
DR Genevisible; Q9ZQ91; AT.
DR GO; GO:0016787; F:hydrolase activity; IBA:GO_Central.
DR GO; GO:0080030; F:methyl indole-3-acetate esterase activity; IEA:UniProtKB-EC.
DR Gene3D; 3.40.50.1820; -; 1.
DR InterPro; IPR029058; AB_hydrolase.
DR InterPro; IPR013094; AB_hydrolase_3.
DR Pfam; PF07859; Abhydrolase_3; 1.
DR SUPFAM; SSF53474; SSF53474; 1.
PE 2: Evidence at transcript level;
KW Acetylation; Hydrolase; Reference proteome; Serine esterase.
FT CHAIN 1..312
FT /note="Probable carboxylesterase 7"
FT /id="PRO_0000402553"
FT MOTIF 75..77
FT /note="Involved in the stabilization of the negatively
FT charged intermediate by the formation of the oxyanion hole"
FT /evidence="ECO:0000250|UniProtKB:Q5NUF3"
FT ACT_SITE 159
FT /evidence="ECO:0000250|UniProtKB:Q5NUF3"
FT ACT_SITE 255
FT /evidence="ECO:0000250|UniProtKB:Q5NUF3"
FT ACT_SITE 287
FT /evidence="ECO:0000250|UniProtKB:Q5NUF3"
FT MOD_RES 1
FT /note="N-acetylmethionine"
FT /evidence="ECO:0000250|UniProtKB:Q9SMN0"
SQ SEQUENCE 312 AA; 34750 MW; 1D9B933F2BE9DD78 CRC64;
MDSVIAFDRS PMFRVYKSGR IERLLGETTV PPSLTPQNGV VSKDIIHSPE KNLSLRIYLP
EKVTVKKLPI LIYFHGGGFI IETAFSPPYH TFLTSAVAAA NCLAISVNYR RAPEFPVPIP
YEDSWDSLKW VLTHITGTGP ETWINKHGDF GKVFLAGDSA GGNISHHLTM RAKKEKLCDS
LISGIILIHP YFWSKTPIDE FEVRDVGKTK GVEGSWRVAS PNSKQGVDDP WLNVVGSDPS
GLGCGRVLVM VAGDDLFVRQ GWCYAEKLKK SGWEGEVEVM ETKNEGHVFH LKNPNSDNAR
QVVKKLEEFI NK
