CXE8_ARATH
ID CXE8_ARATH Reviewed; 329 AA.
AC O64640; Q8LAV3;
DT 11-JAN-2011, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1998, sequence version 1.
DT 03-AUG-2022, entry version 129.
DE RecName: Full=Probable carboxylesterase 8;
DE AltName: Full=AtCXE8;
DE EC=3.1.1.1;
GN Name=CXE8; OrderedLocusNames=At2g45600; ORFNames=17K2.13;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Shinn P., Chen H., Cheuk R.F., Kim C.J., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (DEC-2004) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP TISSUE SPECIFICITY, GENE FAMILY, AND NOMENCLATURE.
RX PubMed=14738307; DOI=10.1007/s00239-003-2492-8;
RA Marshall S.D., Putterill J.J., Plummer K.M., Newcomb R.D.;
RT "The carboxylesterase gene family from Arabidopsis thaliana.";
RL J. Mol. Evol. 57:487-500(2003).
CC -!- FUNCTION: Carboxylesterase acting on esters with varying acyl chain
CC length. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a carboxylic ester + H2O = a carboxylate + an alcohol + H(+);
CC Xref=Rhea:RHEA:21164, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:29067, ChEBI:CHEBI:30879, ChEBI:CHEBI:33308; EC=3.1.1.1;
CC -!- TISSUE SPECIFICITY: Expressed in leaves, stems, flowers and siliques.
CC {ECO:0000269|PubMed:14738307}.
CC -!- SIMILARITY: Belongs to the 'GDXG' lipolytic enzyme family.
CC {ECO:0000305}.
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DR EMBL; AC003680; AAC06164.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC10575.1; -; Genomic_DNA.
DR EMBL; AY058862; AAL24249.1; -; mRNA.
DR EMBL; BT020409; AAV97800.1; -; mRNA.
DR EMBL; AY087590; AAM65132.1; -; mRNA.
DR PIR; T00873; T00873.
DR RefSeq; NP_566047.1; NM_130122.3.
DR AlphaFoldDB; O64640; -.
DR SMR; O64640; -.
DR STRING; 3702.AT2G45600.1; -.
DR ESTHER; arath-CXE8; Plant_carboxylesterase.
DR MEROPS; S09.A07; -.
DR iPTMnet; O64640; -.
DR PaxDb; O64640; -.
DR PRIDE; O64640; -.
DR ProteomicsDB; 220329; -.
DR EnsemblPlants; AT2G45600.1; AT2G45600.1; AT2G45600.
DR GeneID; 819168; -.
DR Gramene; AT2G45600.1; AT2G45600.1; AT2G45600.
DR KEGG; ath:AT2G45600; -.
DR Araport; AT2G45600; -.
DR TAIR; locus:2043644; AT2G45600.
DR eggNOG; KOG1515; Eukaryota.
DR HOGENOM; CLU_012494_22_1_1; -.
DR InParanoid; O64640; -.
DR OMA; IQGLIMN; -.
DR OrthoDB; 1263520at2759; -.
DR PhylomeDB; O64640; -.
DR BioCyc; ARA:AT2G45600-MON; -.
DR PRO; PR:O64640; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; O64640; baseline and differential.
DR Genevisible; O64640; AT.
DR GO; GO:0080030; F:methyl indole-3-acetate esterase activity; IEA:UniProtKB-EC.
DR Gene3D; 3.40.50.1820; -; 1.
DR InterPro; IPR029058; AB_hydrolase.
DR InterPro; IPR013094; AB_hydrolase_3.
DR InterPro; IPR002168; Lipase_GDXG_HIS_AS.
DR Pfam; PF07859; Abhydrolase_3; 1.
DR SUPFAM; SSF53474; SSF53474; 1.
DR PROSITE; PS01173; LIPASE_GDXG_HIS; 1.
PE 2: Evidence at transcript level;
KW Hydrolase; Reference proteome; Serine esterase.
FT CHAIN 1..329
FT /note="Probable carboxylesterase 8"
FT /id="PRO_0000402554"
FT MOTIF 73..75
FT /note="Involved in the stabilization of the negatively
FT charged intermediate by the formation of the oxyanion hole"
FT /evidence="ECO:0000250|UniProtKB:Q5NUF3"
FT ACT_SITE 161
FT /evidence="ECO:0000250|UniProtKB:Q5NUF3"
FT ACT_SITE 264
FT /evidence="ECO:0000250|UniProtKB:Q5NUF3"
FT ACT_SITE 294
FT /evidence="ECO:0000250|UniProtKB:Q5NUF3"
FT CONFLICT 208
FT /note="D -> Y (in Ref. 5; AAM65132)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 329 AA; 36424 MW; 32E3334567EC8147 CRC64;
MEAPPPSSDP YKFLNITLNS DGSLTRHRDF PKLPPTEQSK DIPLNQTNNT FIRIFKPRNI
PPESKLPILV YFHGGGFILY SAASAPFHES CTKMADRLQT IILSVEYRLA PEHRLPAAYE
DAVEAILWLR DQARGPINGG DCDTWLKDGV DFSKCYVMGS SSGGNIVYNV ALRVVDTDLS
PVKIQGLIMN QAFFGGVEPS DSESRLKDDK ICPLPATHLL WSLCLPDGVD RDHVYSNPIK
SSGPQEKDKM GRFPSTLING YGGDPLVDRQ RHVAEMLKGR GVHVETRFDK DGFHACELFD
GNKAKALYET VEAFMKSCSS TGPSSNSNM
