ID   CXE9_ARATH              Reviewed;         324 AA.
AC   O64641;
DT   11-JAN-2011, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1998, sequence version 1.
DT   03-AUG-2022, entry version 117.
DE   RecName: Full=Probable carboxylesterase 9;
DE   AltName: Full=AtCXE9;
DE            EC=3.1.1.1;
GN   Name=CXE9; OrderedLocusNames=At2g45610; ORFNames=F17K2.14;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=10617197; DOI=10.1038/45471;
RA   Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA   Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA   Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA   Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA   Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA   Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA   Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT   "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL   Nature 402:761-768(1999).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   TISSUE SPECIFICITY, GENE FAMILY, AND NOMENCLATURE.
RX   PubMed=14738307; DOI=10.1007/s00239-003-2492-8;
RA   Marshall S.D., Putterill J.J., Plummer K.M., Newcomb R.D.;
RT   "The carboxylesterase gene family from Arabidopsis thaliana.";
RL   J. Mol. Evol. 57:487-500(2003).
CC   -!- FUNCTION: Carboxylesterase acting on esters with varying acyl chain
CC       length. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a carboxylic ester + H2O = a carboxylate + an alcohol + H(+);
CC         Xref=Rhea:RHEA:21164, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:29067, ChEBI:CHEBI:30879, ChEBI:CHEBI:33308; EC=3.1.1.1;
CC   -!- TISSUE SPECIFICITY: Expressed in flowers.
CC       {ECO:0000269|PubMed:14738307}.
CC   -!- SIMILARITY: Belongs to the 'GDXG' lipolytic enzyme family.
CC       {ECO:0000305}.
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DR   EMBL; AC003680; AAC06165.1; -; Genomic_DNA.
DR   EMBL; CP002685; AEC10576.1; -; Genomic_DNA.
DR   PIR; T00874; T00874.
DR   RefSeq; NP_182085.1; NM_130123.2.
DR   AlphaFoldDB; O64641; -.
DR   SMR; O64641; -.
DR   STRING; 3702.AT2G45610.1; -.
DR   ESTHER; arath-At2g45610; Plant_carboxylesterase.
DR   MEROPS; S09.A11; -.
DR   PaxDb; O64641; -.
DR   PRIDE; O64641; -.
DR   ProteomicsDB; 220517; -.
DR   EnsemblPlants; AT2G45610.1; AT2G45610.1; AT2G45610.
DR   GeneID; 819169; -.
DR   Gramene; AT2G45610.1; AT2G45610.1; AT2G45610.
DR   KEGG; ath:AT2G45610; -.
DR   Araport; AT2G45610; -.
DR   TAIR; locus:2043654; AT2G45610.
DR   eggNOG; KOG1515; Eukaryota.
DR   HOGENOM; CLU_012494_22_1_1; -.
DR   InParanoid; O64641; -.
DR   OMA; FRPTKLP; -.
DR   OrthoDB; 1263520at2759; -.
DR   PhylomeDB; O64641; -.
DR   BioCyc; ARA:AT2G45610-MON; -.
DR   PRO; PR:O64641; -.
DR   Proteomes; UP000006548; Chromosome 2.
DR   ExpressionAtlas; O64641; baseline and differential.
DR   Genevisible; O64641; AT.
DR   GO; GO:0080030; F:methyl indole-3-acetate esterase activity; IEA:UniProtKB-EC.
DR   Gene3D; 3.40.50.1820; -; 1.
DR   InterPro; IPR029058; AB_hydrolase.
DR   InterPro; IPR013094; AB_hydrolase_3.
DR   Pfam; PF07859; Abhydrolase_3; 1.
DR   SUPFAM; SSF53474; SSF53474; 1.
PE   2: Evidence at transcript level;
KW   Hydrolase; Reference proteome; Serine esterase.
FT   CHAIN           1..324
FT                   /note="Probable carboxylesterase 9"
FT                   /id="PRO_0000402555"
FT   MOTIF           86..88
FT                   /note="Involved in the stabilization of the negatively
FT                   charged intermediate by the formation of the oxyanion hole"
FT                   /evidence="ECO:0000250|UniProtKB:Q5NUF3"
FT   ACT_SITE        171
FT                   /evidence="ECO:0000250|UniProtKB:Q0ZPV7"
FT   ACT_SITE        272
FT                   /evidence="ECO:0000250|UniProtKB:Q0ZPV7"
FT   ACT_SITE        302
FT                   /evidence="ECO:0000250|UniProtKB:Q0ZPV7"
SQ   SEQUENCE   324 AA;  36018 MW;  531DF08C7E8B1539 CRC64;
     MSRDSPPAFD PYKHLNITIN PNGSCTRHFV WPRVEPDPDP CPGKLAASKD VTINHETGVS
     VRIFRPTNLP SNDNAVARLP IIIHLHGSGW ILYPANSAAN DRCCSQMASE LTVIVVSVHY
     RLPPEHRLPA QYDDALDALL WVKQQVVDST NGEPWLKDYA DFSRCYICGS SNGANIAFQL
     ALRSLDHDLT PLQIDGCVFY QPLFGGKTRT KSELKNFADP VMPVPAVDAM WELSLPVGVD
     RDHRYCNPLG YLPQKEKVGR LGRCLVIGYG GDTSLDRQQD FVNLLVAAGV RVEARFDDAG
     FHSIELVDPR RAVALLNMIR DFIS