CXG1_MOUSE
ID CXG1_MOUSE Reviewed; 396 AA.
AC P28229;
DT 01-DEC-1992, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-1992, sequence version 1.
DT 03-AUG-2022, entry version 179.
DE RecName: Full=Gap junction gamma-1 protein;
DE AltName: Full=Connexin-45;
DE Short=Cx45;
DE AltName: Full=Gap junction alpha-7 protein;
GN Name=Gjc1; Synonyms=Cxn-45, Gja7;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=1322300;
RA Hennemann H., Schwarz H.J., Willecke K.;
RT "Characterization of gap junction genes expressed in F9 embryonic carcinoma
RT cells: molecular cloning of mouse connexin31 and -45 cDNAs.";
RL Eur. J. Cell Biol. 57:51-58(1992).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=12168774; DOI=10.1023/a:1014777111259;
RA Baldridge D., Lecanda F., Shin C.S., Stains J., Civitelli R.;
RT "Sequence and structure of the mouse connexin45 gene.";
RL Biosci. Rep. 21:683-689(2001).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Embryo;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP INTERACTION WITH CNST.
RX PubMed=19864490; DOI=10.1093/hmg/ddp490;
RA del Castillo F.J., Cohen-Salmon M., Charollais A., Caille D., Lampe P.D.,
RA Chavrier P., Meda P., Petit C.;
RT "Consortin, a trans-Golgi network cargo receptor for the plasma membrane
RT targeting and recycling of connexins.";
RL Hum. Mol. Genet. 19:262-275(2010).
CC -!- FUNCTION: One gap junction consists of a cluster of closely packed
CC pairs of transmembrane channels, the connexons, through which materials
CC of low MW diffuse from one cell to a neighboring cell.
CC -!- SUBUNIT: A connexon is composed of a hexamer of connexins. Interacts
CC with CNST. {ECO:0000269|PubMed:19864490}.
CC -!- INTERACTION:
CC P28229; P23242: Gja1; NbExp=2; IntAct=EBI-1767271, EBI-298630;
CC -!- SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. Cell
CC junction, gap junction.
CC -!- SIMILARITY: Belongs to the connexin family. Gamma-type subfamily.
CC {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; X63100; CAA44814.1; -; mRNA.
DR EMBL; AJ300716; CAC21557.1; -; Genomic_DNA.
DR EMBL; BC050840; AAH50840.1; -; mRNA.
DR EMBL; BC071230; AAH71230.1; -; mRNA.
DR CCDS; CCDS25504.1; -.
DR PIR; S23589; S23589.
DR RefSeq; NP_001152854.1; NM_001159382.1.
DR RefSeq; NP_001152855.1; NM_001159383.1.
DR RefSeq; NP_032148.1; NM_008122.3.
DR RefSeq; XP_006532289.1; XM_006532226.3.
DR RefSeq; XP_006532290.1; XM_006532227.3.
DR RefSeq; XP_011247038.1; XM_011248736.2.
DR AlphaFoldDB; P28229; -.
DR BMRB; P28229; -.
DR SMR; P28229; -.
DR BioGRID; 199928; 1.
DR IntAct; P28229; 5.
DR MINT; P28229; -.
DR STRING; 10090.ENSMUSP00000102690; -.
DR iPTMnet; P28229; -.
DR PhosphoSitePlus; P28229; -.
DR PaxDb; P28229; -.
DR PeptideAtlas; P28229; -.
DR PRIDE; P28229; -.
DR ProteomicsDB; 279225; -.
DR Antibodypedia; 29882; 359 antibodies from 33 providers.
DR DNASU; 14615; -.
DR Ensembl; ENSMUST00000068933; ENSMUSP00000066901; ENSMUSG00000034520.
DR Ensembl; ENSMUST00000092567; ENSMUSP00000090228; ENSMUSG00000034520.
DR Ensembl; ENSMUST00000107075; ENSMUSP00000102690; ENSMUSG00000034520.
DR GeneID; 14615; -.
DR KEGG; mmu:14615; -.
DR UCSC; uc007lsk.2; mouse.
DR CTD; 10052; -.
DR MGI; MGI:95718; Gjc1.
DR VEuPathDB; HostDB:ENSMUSG00000034520; -.
DR eggNOG; ENOG502QV2G; Eukaryota.
DR GeneTree; ENSGT01050000244833; -.
DR HOGENOM; CLU_037388_0_0_1; -.
DR InParanoid; P28229; -.
DR OMA; VRWKQHR; -.
DR OrthoDB; 692491at2759; -.
DR PhylomeDB; P28229; -.
DR TreeFam; TF329606; -.
DR Reactome; R-MMU-112303; Electric Transmission Across Gap Junctions.
DR Reactome; R-MMU-190861; Gap junction assembly.
DR BioGRID-ORCS; 14615; 3 hits in 77 CRISPR screens.
DR ChiTaRS; Gjc1; mouse.
DR PRO; PR:P28229; -.
DR Proteomes; UP000000589; Chromosome 11.
DR RNAct; P28229; protein.
DR Bgee; ENSMUSG00000034520; Expressed in ureter smooth muscle and 264 other tissues.
DR ExpressionAtlas; P28229; baseline and differential.
DR Genevisible; P28229; MM.
DR GO; GO:0005922; C:connexin complex; IGI:MGI.
DR GO; GO:0005921; C:gap junction; IDA:MGI.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0045202; C:synapse; IEA:GOC.
DR GO; GO:0005243; F:gap junction channel activity; ISO:MGI.
DR GO; GO:0086077; F:gap junction channel activity involved in AV node cell-bundle of His cell electrical coupling; IMP:BHF-UCL.
DR GO; GO:0005216; F:ion channel activity; IGI:MGI.
DR GO; GO:0086053; P:AV node cell to bundle of His cell communication by electrical coupling; IMP:BHF-UCL.
DR GO; GO:0048738; P:cardiac muscle tissue development; IMP:MGI.
DR GO; GO:0048468; P:cell development; IMP:MGI.
DR GO; GO:0007267; P:cell-cell signaling; IMP:MGI.
DR GO; GO:0007268; P:chemical synaptic transmission; IMP:MGI.
DR GO; GO:0016264; P:gap junction assembly; IMP:BHF-UCL.
DR GO; GO:0007507; P:heart development; IMP:MGI.
DR GO; GO:0001570; P:vasculogenesis; IMP:MGI.
DR GO; GO:0007601; P:visual perception; IMP:MGI.
DR DisProt; DP01464; -.
DR Gene3D; 1.20.1440.80; -; 1.
DR InterPro; IPR000500; Connexin.
DR InterPro; IPR002265; Connexin45.
DR InterPro; IPR019570; Connexin_CCC.
DR InterPro; IPR017990; Connexin_CS.
DR InterPro; IPR013092; Connexin_N.
DR InterPro; IPR038359; Connexin_N_sf.
DR PANTHER; PTHR11984; PTHR11984; 1.
DR Pfam; PF00029; Connexin; 1.
DR PRINTS; PR00206; CONNEXIN.
DR PRINTS; PR01136; CONNEXINA6.
DR SMART; SM00037; CNX; 1.
DR SMART; SM01089; Connexin_CCC; 1.
DR PROSITE; PS00407; CONNEXINS_1; 1.
DR PROSITE; PS00408; CONNEXINS_2; 1.
PE 1: Evidence at protein level;
KW Cell junction; Cell membrane; Gap junction; Membrane; Reference proteome;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..396
FT /note="Gap junction gamma-1 protein"
FT /id="PRO_0000057827"
FT TOPO_DOM 1..22
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 23..45
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 46..75
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 76..95
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 96..175
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 176..198
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 199..228
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 229..248
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 249..396
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 145..165
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 357..396
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 375..396
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 396 AA; 45666 MW; 9CD5721BB071B65C CRC64;
MSWSFLTRLL EEIHNHSTFV GKIWLTVLIV FRIVLTAVGG ESIYYDEQSK FVCNTEQPGC
ENVCYDAFAP LSHVRFWVFQ IILVATPSVM YLGYAIHKIA KMEHGEADKK AARSKPYAMR
WKQHRALEET EEDHEEDPMM YPEMELESEK ENKEQSQPKP KHDGRRRIRE DGLMKIYVLQ
LLARTVFEVG FLIGQYFLYG FQVHPFYVCS RLPCPHKIDC FISRPTEKTI FLLIMYGVTG
LCLLLNIWEM LHLGFGTIRD SLNSKRRELD DPGAYNYPFT WNTPSAPPGY NIAVKPDQIQ
YTELSNAKIA YKQNKANIAQ EQQYGSHEEH LPADLETLQR EIRMAQERLD LAIQAYHHQN
NPHGPREKKA KVGSKSGSNK SSISSKSGDG KTSVWI
