ID   CXPA_CONCF              Reviewed;          23 AA.
AC   P0DM45;
DT   26-JUN-2013, integrated into UniProtKB/Swiss-Prot.
DT   26-JUN-2013, sequence version 1.
DT   25-MAY-2022, entry version 14.
DE   RecName: Full=Conotoxin as25a {ECO:0000303|PubMed:23474143};
DE   AltName: Full=As25b {ECO:0000303|PubMed:23474143};
OS   Conus cancellatus (Cancellate cone) (Conus austini).
OC   Eukaryota; Metazoa; Spiralia; Lophotrochozoa; Mollusca; Gastropoda;
OC   Caenogastropoda; Neogastropoda; Conoidea; Conidae; Conus; Dauciconus.
OX   NCBI_TaxID=289020;
RN   [1]
RP   PROTEIN SEQUENCE, FUNCTION, BIOASSAY, HYDROXYLATION AT PRO-4 AND PRO-23,
RP   AMIDATION AT PRO-23, MASS SPECTROMETRY, AND SUBCELLULAR LOCATION.
RC   TISSUE=Venom;
RX   PubMed=23474143; DOI=10.1016/j.peptides.2013.02.016;
RA   Aguilar M.B., Zugasti-Cruz A., Falcon A., Batista C.V., Olivera B.M.,
RA   Heimer de la Cotera E.P.;
RT   "A novel arrangement of Cys residues in a paralytic peptide of Conus
RT   cancellatus (jr. syn.: Conus austini), a worm-hunting snail from the Gulf
RT   of Mexico.";
RL   Peptides 41:38-44(2013).
CC   -!- FUNCTION: Upon intracranial injection in mice, as25a (the toxin without
CC       the two 4-hydroxyprolines) provokes paralysis of the hind limbs and
CC       death with a dose of 240 pmol. {ECO:0000269|PubMed:23474143}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:23474143}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom duct.
CC       {ECO:0000305|PubMed:23474143}.
CC   -!- DOMAIN: The cysteine framework is XXV (C-C-C-C-CC). {ECO:0000305}.
CC   -!- PTM: The name as25b given in PubMed:23474143 corresponds to the
CC       hydroxylated peptide (PubMed:23474143). The amidation of the C-terminus
CC       of this hydroxylated peptide is not directly confirmed
CC       (PubMed:23474143). {ECO:0000269|PubMed:23474143}.
CC   -!- PTM: Contains 3 disulfide bonds. {ECO:0000269|PubMed:23474143}.
CC   -!- MASS SPECTROMETRY: Mass=2644.12; Method=MALDI; Note=Monoisotopic mass.;
CC       Evidence={ECO:0000269|PubMed:23474143};
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DR   AlphaFoldDB; P0DM45; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Amidation; Direct protein sequencing; Disulfide bond; Hydroxylation;
KW   Neurotoxin; Secreted; Toxin.
FT   PEPTIDE         1..23
FT                   /note="Conotoxin as25a"
FT                   /evidence="ECO:0000269|PubMed:23474143"
FT                   /id="PRO_0000422883"
FT   MOD_RES         4
FT                   /note="4-hydroxyproline; partial"
FT                   /evidence="ECO:0000269|PubMed:23474143"
FT   MOD_RES         23
FT                   /note="4-hydroxyproline; partial; alternate"
FT                   /evidence="ECO:0000269|PubMed:23474143"
FT   MOD_RES         23
FT                   /note="Proline amide; alternate"
FT                   /evidence="ECO:0000269|PubMed:23474143"
SQ   SEQUENCE   23 AA;  2653 MW;  4A2D1FA5094F38B2 CRC64;
     CKCPSCNFND VTENCKCCIF RQP