CXR32_DANRE
ID CXR32_DANRE Reviewed; 378 AA.
AC E9QJ73; B0S589; Q5U387;
DT 05-OCT-2016, integrated into UniProtKB/Swiss-Prot.
DT 11-MAY-2016, sequence version 2.
DT 03-AUG-2022, entry version 67.
DE RecName: Full=C-X-C chemokine receptor type 3-2 {ECO:0000305};
GN Name=cxcr3.2 {ECO:0000312|ZFIN:ZDB-GENE-041114-186};
OS Danio rerio (Zebrafish) (Brachydanio rerio).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC Danionidae; Danioninae; Danio.
OX NCBI_TaxID=7955 {ECO:0000312|Proteomes:UP000000437};
RN [1] {ECO:0000312|Proteomes:UP000000437}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Tuebingen {ECO:0000312|Proteomes:UP000000437};
RX PubMed=23594743; DOI=10.1038/nature12111;
RA Howe K., Clark M.D., Torroja C.F., Torrance J., Berthelot C., Muffato M.,
RA Collins J.E., Humphray S., McLaren K., Matthews L., McLaren S., Sealy I.,
RA Caccamo M., Churcher C., Scott C., Barrett J.C., Koch R., Rauch G.J.,
RA White S., Chow W., Kilian B., Quintais L.T., Guerra-Assuncao J.A., Zhou Y.,
RA Gu Y., Yen J., Vogel J.H., Eyre T., Redmond S., Banerjee R., Chi J., Fu B.,
RA Langley E., Maguire S.F., Laird G.K., Lloyd D., Kenyon E., Donaldson S.,
RA Sehra H., Almeida-King J., Loveland J., Trevanion S., Jones M., Quail M.,
RA Willey D., Hunt A., Burton J., Sims S., McLay K., Plumb B., Davis J.,
RA Clee C., Oliver K., Clark R., Riddle C., Elliot D., Threadgold G.,
RA Harden G., Ware D., Begum S., Mortimore B., Kerry G., Heath P.,
RA Phillimore B., Tracey A., Corby N., Dunn M., Johnson C., Wood J., Clark S.,
RA Pelan S., Griffiths G., Smith M., Glithero R., Howden P., Barker N.,
RA Lloyd C., Stevens C., Harley J., Holt K., Panagiotidis G., Lovell J.,
RA Beasley H., Henderson C., Gordon D., Auger K., Wright D., Collins J.,
RA Raisen C., Dyer L., Leung K., Robertson L., Ambridge K., Leongamornlert D.,
RA McGuire S., Gilderthorp R., Griffiths C., Manthravadi D., Nichol S.,
RA Barker G., Whitehead S., Kay M., Brown J., Murnane C., Gray E.,
RA Humphries M., Sycamore N., Barker D., Saunders D., Wallis J., Babbage A.,
RA Hammond S., Mashreghi-Mohammadi M., Barr L., Martin S., Wray P.,
RA Ellington A., Matthews N., Ellwood M., Woodmansey R., Clark G., Cooper J.,
RA Tromans A., Grafham D., Skuce C., Pandian R., Andrews R., Harrison E.,
RA Kimberley A., Garnett J., Fosker N., Hall R., Garner P., Kelly D., Bird C.,
RA Palmer S., Gehring I., Berger A., Dooley C.M., Ersan-Urun Z., Eser C.,
RA Geiger H., Geisler M., Karotki L., Kirn A., Konantz J., Konantz M.,
RA Oberlander M., Rudolph-Geiger S., Teucke M., Lanz C., Raddatz G.,
RA Osoegawa K., Zhu B., Rapp A., Widaa S., Langford C., Yang F.,
RA Schuster S.C., Carter N.P., Harrow J., Ning Z., Herrero J., Searle S.M.,
RA Enright A., Geisler R., Plasterk R.H., Lee C., Westerfield M.,
RA de Jong P.J., Zon L.I., Postlethwait J.H., Nusslein-Volhard C.,
RA Hubbard T.J., Roest Crollius H., Rogers J., Stemple D.L.;
RT "The zebrafish reference genome sequence and its relationship to the human
RT genome.";
RL Nature 496:498-503(2013).
RN [2] {ECO:0000312|EMBL:AAH85659.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RG NIH - Zebrafish Gene Collection (ZGC) project;
RL Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases.
RN [3] {ECO:0000305}
RP FUNCTION, DEVELOPMENTAL STAGE, AND DISRUPTION PHENOTYPE.
RX PubMed=20424185; DOI=10.1182/blood-2010-01-262873;
RA Zakrzewska A., Cui C., Stockhammer O.W., Benard E.L., Spaink H.P.,
RA Meijer A.H.;
RT "Macrophage-specific gene functions in Spi1-directed innate immunity.";
RL Blood 116:E1-E11(2010).
RN [4] {ECO:0000305}
RP FUNCTION, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, AND DISRUPTION
RP PHENOTYPE.
RX PubMed=25573892; DOI=10.1242/dmm.017756;
RA Torraca V., Cui C., Boland R., Bebelman J.P., van der Sar A.M., Smit M.J.,
RA Siderius M., Spaink H.P., Meijer A.H.;
RT "The CXCR3-CXCL11 signaling axis mediates macrophage recruitment and
RT dissemination of mycobacterial infection.";
RL Dis. Model. Mech. 8:253-269(2015).
CC -!- FUNCTION: Receptor for the C-X-C chemokines cxcl11.1 and cxcl11.6
CC (PubMed:25573892). Promotes macrophage chemotaxis to sites of bacterial
CC infection (PubMed:20424185, PubMed:25573892).
CC {ECO:0000269|PubMed:20424185, ECO:0000269|PubMed:25573892}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305|PubMed:25573892};
CC Multi-pass membrane protein {ECO:0000255}.
CC -!- DEVELOPMENTAL STAGE: Detected in developing macrophages at 28 hours
CC post-fertilization (PubMed:20424185). Continues to be expressed in
CC macrophages at 2 and 6 days post-fertilization (dpf) (PubMed:25573892).
CC Also detected in neutrophils at 2 and 6 dpf (PubMed:25573892).
CC {ECO:0000269|PubMed:20424185, ECO:0000269|PubMed:25573892}.
CC -!- DISRUPTION PHENOTYPE: Viable with no significant effect on survival
CC (PubMed:25573892). Macrophage and neutrophil counts are normal
CC (PubMed:25573892). Macrophage migration in response to cxcr3.2-
CC independent stimuli is not affected, although the basal migration rate
CC is reduced (PubMed:25573892). Macrophage migration to sites of
CC bacterial infection by S.typhimurium or M.marinum is reduced
CC (PubMed:20424185, PubMed:25573892). Dissemination of bacterial
CC infection to distant sites and granuloma formation is also reduced
CC (PubMed:25573892). {ECO:0000269|PubMed:20424185,
CC ECO:0000269|PubMed:25573892}.
CC -!- SIMILARITY: Belongs to the G-protein coupled receptor 1 family.
CC {ECO:0000255|PROSITE-ProRule:PRU00521}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; BX005342; CAQ13805.1; -; Genomic_DNA.
DR EMBL; CR848719; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC085659; AAH85659.1; -; mRNA.
DR RefSeq; NP_001007315.1; NM_001007314.1.
DR AlphaFoldDB; E9QJ73; -.
DR SMR; E9QJ73; -.
DR STRING; 7955.ENSDARP00000122984; -.
DR PaxDb; E9QJ73; -.
DR Ensembl; ENSDART00000145727; ENSDARP00000122984; ENSDARG00000041041.
DR GeneID; 791973; -.
DR KEGG; dre:791973; -.
DR CTD; 791973; -.
DR ZFIN; ZDB-GENE-041114-186; cxcr3.2.
DR eggNOG; KOG3656; Eukaryota.
DR GeneTree; ENSGT01050000244848; -.
DR HOGENOM; CLU_1264104_0_0_1; -.
DR OMA; STCHAQF; -.
DR OrthoDB; 794531at2759; -.
DR PhylomeDB; E9QJ73; -.
DR PRO; PR:E9QJ73; -.
DR Proteomes; UP000000437; Genome assembly.
DR Proteomes; UP000814640; Chromosome 16.
DR Bgee; ENSDARG00000041041; Expressed in granulocyte and 16 other tissues.
DR GO; GO:0009897; C:external side of plasma membrane; IBA:GO_Central.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0019957; F:C-C chemokine binding; IBA:GO_Central.
DR GO; GO:0016493; F:C-C chemokine receptor activity; IBA:GO_Central.
DR GO; GO:0019722; P:calcium-mediated signaling; IBA:GO_Central.
DR GO; GO:0060326; P:cell chemotaxis; IBA:GO_Central.
DR GO; GO:0070098; P:chemokine-mediated signaling pathway; IGI:ZFIN.
DR GO; GO:0006955; P:immune response; IBA:GO_Central.
DR GO; GO:0045087; P:innate immune response; IEA:UniProtKB-KW.
DR GO; GO:0002522; P:leukocyte migration involved in immune response; IMP:ZFIN.
DR GO; GO:0002281; P:macrophage activation involved in immune response; IMP:ZFIN.
DR GO; GO:0048246; P:macrophage chemotaxis; IGI:ZFIN.
DR GO; GO:0007204; P:positive regulation of cytosolic calcium ion concentration; IBA:GO_Central.
DR GO; GO:0009617; P:response to bacterium; IMP:ZFIN.
DR InterPro; IPR000248; ATII_rcpt.
DR InterPro; IPR000276; GPCR_Rhodpsn.
DR InterPro; IPR017452; GPCR_Rhodpsn_7TM.
DR Pfam; PF00001; 7tm_1; 1.
DR PRINTS; PR00241; ANGIOTENSINR.
DR PRINTS; PR00237; GPCRRHODOPSN.
DR PROSITE; PS00237; G_PROTEIN_RECEP_F1_1; 1.
DR PROSITE; PS50262; G_PROTEIN_RECEP_F1_2; 1.
PE 2: Evidence at transcript level;
KW Cell membrane; Chemotaxis; Disulfide bond; G-protein coupled receptor;
KW Glycoprotein; Immunity; Innate immunity; Membrane; Receptor;
KW Reference proteome; Transducer; Transmembrane; Transmembrane helix.
FT CHAIN 1..378
FT /note="C-X-C chemokine receptor type 3-2"
FT /id="PRO_0000437521"
FT TOPO_DOM 1..47
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 48..68
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 69..86
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 87..107
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 108..121
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 122..142
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 143..164
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 165..185
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 186..212
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 213..233
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 234..253
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 254..274
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 275..304
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 305..325
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 326..378
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT CARBOHYD 3
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 19
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT DISULFID 120..199
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00521"
FT CONFLICT 8
FT /note="A -> T (in Ref. 2; AAH85659)"
FT /evidence="ECO:0000305"
FT CONFLICT 19..21
FT /note="NST -> SSA (in Ref. 2; AAH85659)"
FT /evidence="ECO:0000305"
FT CONFLICT 29
FT /note="Y -> N (in Ref. 2; AAH85659)"
FT /evidence="ECO:0000305"
FT CONFLICT 36
FT /note="T -> R (in Ref. 2; AAH85659)"
FT /evidence="ECO:0000305"
FT CONFLICT 52
FT /note="L -> M (in Ref. 2; AAH85659)"
FT /evidence="ECO:0000305"
FT CONFLICT 93
FT /note="V -> I (in Ref. 1; CAQ13805)"
FT /evidence="ECO:0000305"
FT CONFLICT 190
FT /note="E -> Q (in Ref. 2; AAH85659)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 378 AA; 42263 MW; 45B3A05C18E7923B CRC64;
MDNSTTAAEV SAPTDYDYNS TSYDDDNPYA APCSLTETWN FLGRFAPVAY ILVFILALVG
NILVLCVIRR YRQSRHSPCS FSLTDTFLLH LAVSDLLLAA TLPFFAVEWI SEWVFGKVMC
KITGALFSLN VYCGVLFLAC ISFDRYLAIV HAINISWRRK TCHAQLACAF IWVICLGLSM
VDMHFRDLVE IPGMNRMVCQ IVYSEQYSKQ WQIGMQLVSM VLGFILPLLV MLYCYLHIFK
ALCHATRRQK RRSLRLIISL VIVFVISWAP YNALRMTDSL QMLGVIVKSC ALNNVLDVGI
LVTESLGLAH CALNPLLYGL VGVKFRRELA QMCKAALGPQ GCLGLVGWAN GRGSSTRRPT
GSFSSVETEN TSYFSVMA
