ID   CXX_CONTE               Reviewed;          82 AA.
AC   Q5I4E6;
DT   26-APR-2005, integrated into UniProtKB/Swiss-Prot.
DT   15-FEB-2005, sequence version 1.
DT   25-MAY-2022, entry version 34.
DE   RecName: Full=Conotoxin Gla-TxX;
DE   Flags: Precursor;
OS   Conus textile (Cloth-of-gold cone).
OC   Eukaryota; Metazoa; Spiralia; Lophotrochozoa; Mollusca; Gastropoda;
OC   Caenogastropoda; Neogastropoda; Conoidea; Conidae; Conus; Cylinder.
OX   NCBI_TaxID=6494;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 26-72,
RP   GAMMA-CARBOXYGLUTAMATION AT GLU-30; GLU-34; GLU-37; GLU-40 AND GLU-41,
RP   AMIDATION AT ASN-72, AND MASS SPECTROMETRY.
RC   TISSUE=Venom, and Venom duct;
RX   PubMed=15966739; DOI=10.1021/bi0503293;
RA   Brown M.A., Begley G.S., Czerwiec E., Stenberg L.M., Jacobs M.,
RA   Kalume D.E., Roepstorff P., Stenflo J., Furie B.C., Furie B.;
RT   "Precursors of novel Gla-containing conotoxins contain a carboxy-terminal
RT   recognition site that directs gamma-carboxylation.";
RL   Biochemistry 44:9150-9159(2005).
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom duct.
CC   -!- DOMAIN: The cysteine framework is XII (C-C-C-C-CC-C-C).
CC   -!- PTM: Contains 4 disulfide bonds.
CC   -!- MASS SPECTROMETRY: Mass=5603.3; Method=MALDI;
CC       Evidence={ECO:0000269|PubMed:15966739};
CC   -!- SIMILARITY: Belongs to the conotoxin I2 superfamily. {ECO:0000305}.
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DR   EMBL; AY856069; AAW50949.1; -; mRNA.
DR   AlphaFoldDB; Q5I4E6; -.
DR   ConoServer; 1056; TxX precursor.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Amidation; Cleavage on pair of basic residues; Direct protein sequencing;
KW   Disulfide bond; Gamma-carboxyglutamic acid; Secreted; Signal; Toxin.
FT   SIGNAL          1..25
FT                   /evidence="ECO:0000250"
FT   PEPTIDE         26..72
FT                   /note="Conotoxin Gla-TxX"
FT                   /id="PRO_0000035124"
FT   PROPEP          77..82
FT                   /id="PRO_0000035125"
FT   MOD_RES         30
FT                   /note="4-carboxyglutamate"
FT                   /evidence="ECO:0000269|PubMed:15966739"
FT   MOD_RES         34
FT                   /note="4-carboxyglutamate"
FT                   /evidence="ECO:0000269|PubMed:15966739"
FT   MOD_RES         37
FT                   /note="4-carboxyglutamate"
FT                   /evidence="ECO:0000269|PubMed:15966739"
FT   MOD_RES         40
FT                   /note="4-carboxyglutamate"
FT                   /evidence="ECO:0000269|PubMed:15966739"
FT   MOD_RES         41
FT                   /note="4-carboxyglutamate"
FT                   /evidence="ECO:0000269|PubMed:15966739"
FT   MOD_RES         72
FT                   /note="Asparagine amide"
FT                   /evidence="ECO:0000269|PubMed:15966739"
SQ   SEQUENCE   82 AA;  9199 MW;  DB3852240E9BDFB9 CRC64;
     MSGHTSVSFL LLSIVALGMV ATVICSCDSE FSSEFCERPE ESCSCSTHTC CHWARRDQCM
     KPQRCISAQK GNGRRRLIHM QK