ID   CY081_POSPM             Reviewed;         547 AA.
AC   F1SY77;
DT   02-DEC-2020, integrated into UniProtKB/Swiss-Prot.
DT   31-MAY-2011, sequence version 1.
DT   03-AUG-2022, entry version 29.
DE   RecName: Full=Cytochrome P450 monooxygenase 81 {ECO:0000303|PubMed:21938516};
DE            EC=1.-.-.- {ECO:0000269|PubMed:21938516};
GN   Name=CYP081 {ECO:0000303|PubMed:21938516};
GN   Synonyms=CYP5150D18 {ECO:0000303|PubMed:21938516};
OS   Postia placenta (strain ATCC 44394 / Madison 698-R) (Brown rot fungus)
OS   (Poria monticola).
OC   Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes;
OC   Polyporales; Dacryobolaceae; Postia.
OX   NCBI_TaxID=561896;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], IDENTIFICATION, FUNCTION, AND CATALYTIC
RP   ACTIVITY.
RC   STRAIN=ATCC 44394 / Madison 698-R;
RX   PubMed=21938516; DOI=10.1007/s00203-011-0753-2;
RA   Ide M., Ichinose H., Wariishi H.;
RT   "Molecular identification and functional characterization of cytochrome
RT   P450 monooxygenases from the brown-rot basidiomycete Postia placenta.";
RL   Arch. Microbiol. 194:243-253(2012).
CC   -!- FUNCTION: Cytochrome P450 monooxygenase that is able to use
CC       dehydroabietic acid as a substrate for oxidation.
CC       {ECO:0000269|PubMed:21938516}.
CC   -!- COFACTOR:
CC       Name=heme; Xref=ChEBI:CHEBI:30413;
CC         Evidence={ECO:0000250|UniProtKB:P04798};
CC   -!- PATHWAY: Secondary metabolite biosynthesis. {ECO:0000305}.
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000255}; Multi-pass membrane
CC       protein {ECO:0000255}.
CC   -!- SIMILARITY: Belongs to the cytochrome P450 family. {ECO:0000305}.
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DR   EMBL; AB573288; BAK09421.1; -; mRNA.
DR   AlphaFoldDB; F1SY77; -.
DR   SMR; F1SY77; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0020037; F:heme binding; IEA:InterPro.
DR   GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR   GO; GO:0004497; F:monooxygenase activity; IEA:UniProtKB-KW.
DR   GO; GO:0016705; F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen; IEA:InterPro.
DR   Gene3D; 1.10.630.10; -; 1.
DR   InterPro; IPR001128; Cyt_P450.
DR   InterPro; IPR002401; Cyt_P450_E_grp-I.
DR   InterPro; IPR036396; Cyt_P450_sf.
DR   Pfam; PF00067; p450; 1.
DR   PRINTS; PR00463; EP450I.
DR   PRINTS; PR00385; P450.
DR   SUPFAM; SSF48264; SSF48264; 1.
PE   1: Evidence at protein level;
KW   Glycoprotein; Heme; Iron; Membrane; Metal-binding; Monooxygenase;
KW   Oxidoreductase; Transmembrane; Transmembrane helix.
FT   CHAIN           1..547
FT                   /note="Cytochrome P450 monooxygenase 81"
FT                   /id="PRO_0000451364"
FT   TRANSMEM        6..23
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        106..124
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   BINDING         483
FT                   /ligand="heme"
FT                   /ligand_id="ChEBI:CHEBI:30413"
FT                   /ligand_part="Fe"
FT                   /ligand_part_id="ChEBI:CHEBI:18248"
FT                   /note="axial binding residue"
FT                   /evidence="ECO:0000250|UniProtKB:P04798"
FT   CARBOHYD        503
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT   CARBOHYD        516
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
SQ   SEQUENCE   547 AA;  61636 MW;  08AFBB085B39E0BF CRC64;
     MSTDNIPTQL GIAGAVAVLL FLLRRWSSRS TLRNIPGPPP QSQWTGNLKQ WFARDGADFQ
     RDVSFNYGPV AKIHGFLGRP ILYVADPKAL QTILVKEEQV YQETKAFFAM TYLLFGPGLL
     ATAGEKHRKQ RKLLNPVFSI KHMRHMLPIF YGVLHKVRDA ITMRVSDGPQ EIDMLKWMGR
     TALELIGQGG LGYSFDKLVE DGDNEYGRAL KHLQPTLQRI NVLRRLIPYV YKLGPAWFRR
     MVMHYFPLGQ VRDAKEIVDT MQRCSSEIFA SKKIALARGD EAVMKQVGEG KDIMSILMKA
     NSMASEADRI PEEELVAQMS TFLFAATDTT SNTLARILQQ LAIHPDTQQK LREEILAANA
     EEYMAYDDLD ALPLLDGVCR ETLRVFPGVT NLARTPTQDT ILPLSEPVVG TDGTVMREIL
     VPRGTEILIG IQGSNGRKER WGEDSYEWKP ERWLSPLPKT VTENPVPGVY SNLMTFMAGR
     RACIGFKFSE MEMKVVLAVL LSNFTFELTD KPIQWNISGV RYPTVGKDSN VAQLPLKVGL
     YKKPTLQ