CY24A_DICDI
ID CY24A_DICDI Reviewed; 118 AA.
AC Q867X6; Q55GP4;
DT 20-JAN-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2003, sequence version 1.
DT 25-MAY-2022, entry version 76.
DE RecName: Full=Superoxide-generating NADPH oxidase light chain subunit;
DE AltName: Full=Cytochrome b-245 light chain;
DE AltName: Full=p22-phox;
DE Short=p22phox;
GN Name=cybA; ORFNames=DDB_G0267460;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], DEVELOPMENTAL STAGE, AND DISRUPTION
RP PHENOTYPE.
RX PubMed=15950752; DOI=10.1016/j.bbamcr.2005.02.004;
RA Lardy B., Bof M., Aubry L., Paclet M.H., Morel F., Satre M., Klein G.;
RT "NADPH oxidase homologs are required for normal cell differentiation and
RT morphogenesis in Dictyostelium discoideum.";
RL Biochim. Biophys. Acta 1744:199-212(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
CC -!- FUNCTION: Critical component of the membrane-bound oxidase of
CC phagocytes that generates superoxide. {ECO:0000250}.
CC -!- SUBUNIT: Composed of a heavy chain and a light chain. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250|UniProtKB:P13498}.
CC Note=As unassembled monomer may localize to the endoplasmic reticulum.
CC {ECO:0000250|UniProtKB:Q61462}.
CC -!- DEVELOPMENTAL STAGE: Expressed during vegetative stage. Expression
CC decreases during development and disappears from 8 hours.
CC {ECO:0000269|PubMed:15950752}.
CC -!- DISRUPTION PHENOTYPE: Prevents formation of fruiting bodies.
CC {ECO:0000269|PubMed:15950752}.
CC -!- SIMILARITY: Belongs to the p22phox family. {ECO:0000305}.
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DR EMBL; AY221170; AAO62418.1; -; mRNA.
DR EMBL; AY221171; AAO62419.1; -; Genomic_DNA.
DR EMBL; AAFI02000003; EAL73183.1; -; Genomic_DNA.
DR RefSeq; XP_647140.1; XM_642048.1.
DR AlphaFoldDB; Q867X6; -.
DR STRING; 44689.DDB0191314; -.
DR PaxDb; Q867X6; -.
DR EnsemblProtists; EAL73183; EAL73183; DDB_G0267460.
DR GeneID; 8615943; -.
DR KEGG; ddi:DDB_G0267460; -.
DR dictyBase; DDB_G0267460; cybA.
DR eggNOG; ENOG502RHUE; Eukaryota.
DR HOGENOM; CLU_2077485_0_0_1; -.
DR InParanoid; Q867X6; -.
DR OMA; MIGMAAC; -.
DR PhylomeDB; Q867X6; -.
DR Reactome; R-DDI-3299685; Detoxification of Reactive Oxygen Species.
DR Reactome; R-DDI-5668599; RHO GTPases Activate NADPH Oxidases.
DR Reactome; R-DDI-6798695; Neutrophil degranulation.
DR Reactome; R-DDI-9013149; RAC1 GTPase cycle.
DR Reactome; R-DDI-9013423; RAC3 GTPase cycle.
DR PRO; PR:Q867X6; -.
DR Proteomes; UP000002195; Chromosome 1.
DR GO; GO:0043020; C:NADPH oxidase complex; IBA:GO_Central.
DR GO; GO:0020037; F:heme binding; IEA:InterPro.
DR GO; GO:0045087; P:innate immune response; ISS:UniProtKB.
DR GO; GO:0030587; P:sorocarp development; IMP:dictyBase.
DR GO; GO:0042554; P:superoxide anion generation; IBA:GO_Central.
DR InterPro; IPR007732; Cyt_b558_asu.
DR PANTHER; PTHR15168; PTHR15168; 1.
DR Pfam; PF05038; Cytochrom_B558a; 1.
PE 2: Evidence at transcript level;
KW Cell membrane; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..118
FT /note="Superoxide-generating NADPH oxidase light chain
FT subunit"
FT /id="PRO_0000361530"
FT TRANSMEM 9..29
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 36..56
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 62..82
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 83..103
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 118 AA; 13008 MW; 2F07828020A16DBB CRC64;
MGKFKLGNWA AMIGMAACWC LIAGGIMGIW YERRYIAIYS ICVGGVLYPL LYPLSFLGPL
KAIFHQYYVA AALMAGLSVL CYFLVPTMLA AMVMDISAVV FLISAIKGER GDFFDKQD
