ID   CY550_BRADU             Reviewed;         136 AA.
AC   Q45233;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   03-AUG-2022, entry version 134.
DE   RecName: Full=Cytochrome c-550;
DE   AltName: Full=Cytochrome c550;
DE   Flags: Precursor;
GN   Name=cycA; OrderedLocusNames=blr7544;
OS   Bradyrhizobium diazoefficiens (strain JCM 10833 / BCRC 13528 / IAM 13628 /
OS   NBRC 14792 / USDA 110).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Bradyrhizobiaceae; Bradyrhizobium.
OX   NCBI_TaxID=224911;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 66-92 AND
RP   107-130.
RC   STRAIN=CC705 / UW505, and USDA 110spc4;
RX   PubMed=7721713; DOI=10.1128/jb.177.8.2214-2217.1995;
RA   Bott M., Thoeny-Meyer L., Loferer H., Rossbach S., Tully R.E., Keister D.,
RA   Appleby C.A., Hennecke H.;
RT   "Bradyrhizobium japonicum cytochrome c550 is required for nitrate
RT   respiration but not for symbiotic nitrogen fixation.";
RL   J. Bacteriol. 177:2214-2217(1995).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=JCM 10833 / BCRC 13528 / IAM 13628 / NBRC 14792 / USDA 110;
RX   PubMed=12597275; DOI=10.1093/dnares/9.6.189;
RA   Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S.,
RA   Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M.,
RA   Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S.;
RT   "Complete genomic sequence of nitrogen-fixing symbiotic bacterium
RT   Bradyrhizobium japonicum USDA110.";
RL   DNA Res. 9:189-197(2002).
CC   -!- FUNCTION: Plays a role in bacteroid respiration under conditions of
CC       oxygen limitation. Required for electron-transfer during
CC       denitrification.
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       Redox potential:
CC         E(0) is about +280 mV.;
CC   -!- SUBCELLULAR LOCATION: Periplasm.
CC   -!- PTM: Binds 1 heme c group covalently per subunit.
CC   -!- PTM: The N-terminus is blocked.
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DR   EMBL; L39642; AAA74907.1; -; Genomic_DNA.
DR   EMBL; BA000040; BAC52809.1; -; Genomic_DNA.
DR   PIR; A56280; A56280.
DR   RefSeq; NP_774184.1; NC_004463.1.
DR   RefSeq; WP_011090276.1; NZ_CP011360.1.
DR   AlphaFoldDB; Q45233; -.
DR   SMR; Q45233; -.
DR   STRING; 224911.27355827; -.
DR   EnsemblBacteria; BAC52809; BAC52809; BAC52809.
DR   GeneID; 64027300; -.
DR   KEGG; bja:blr7544; -.
DR   PATRIC; fig|224911.44.peg.7647; -.
DR   eggNOG; COG3474; Bacteria.
DR   HOGENOM; CLU_060944_2_1_5; -.
DR   InParanoid; Q45233; -.
DR   OMA; YSDAMKN; -.
DR   PhylomeDB; Q45233; -.
DR   Proteomes; UP000002526; Chromosome.
DR   GO; GO:0042597; C:periplasmic space; IEA:UniProtKB-SubCell.
DR   GO; GO:0009055; F:electron transfer activity; IEA:InterPro.
DR   GO; GO:0020037; F:heme binding; IEA:InterPro.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.760.10; -; 1.
DR   InterPro; IPR009056; Cyt_c-like_dom.
DR   InterPro; IPR036909; Cyt_c-like_dom_sf.
DR   InterPro; IPR002327; Cyt_c_1A/1B.
DR   PANTHER; PTHR11961; PTHR11961; 1.
DR   Pfam; PF00034; Cytochrom_C; 1.
DR   PRINTS; PR00604; CYTCHRMECIAB.
DR   SUPFAM; SSF46626; SSF46626; 1.
DR   PROSITE; PS51007; CYTC; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Electron transport; Heme; Iron; Metal-binding;
KW   Periplasm; Reference proteome; Signal; Transport.
FT   SIGNAL          1..28
FT                   /evidence="ECO:0000255"
FT   CHAIN           29..136
FT                   /note="Cytochrome c-550"
FT                   /id="PRO_0000006522"
FT   BINDING         41
FT                   /ligand="heme c"
FT                   /ligand_id="ChEBI:CHEBI:61717"
FT                   /note="covalent"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00433"
FT   BINDING         44
FT                   /ligand="heme c"
FT                   /ligand_id="ChEBI:CHEBI:61717"
FT                   /note="covalent"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00433"
FT   BINDING         45
FT                   /ligand="heme c"
FT                   /ligand_id="ChEBI:CHEBI:61717"
FT                   /ligand_part="Fe"
FT                   /ligand_part_id="ChEBI:CHEBI:18248"
FT                   /note="axial binding residue"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00433"
FT   BINDING         107
FT                   /ligand="heme c"
FT                   /ligand_id="ChEBI:CHEBI:61717"
FT                   /ligand_part="Fe"
FT                   /ligand_part_id="ChEBI:CHEBI:18248"
FT                   /note="axial binding residue"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00433"
FT   CONFLICT        70..72
FT                   /note="APD -> VEG (in Ref. 1; AA sequence)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        76
FT                   /note="S -> T (in Ref. 1; AA sequence)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        90
FT                   /note="T -> V (in Ref. 1; AA sequence)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        123..127
FT                   /note="TFVSQ -> AYVAD (in Ref. 1; AA sequence)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   136 AA;  14432 MW;  DF889E566245E162 CRC64;
     MTKLTFGALV ALAMTAAAST AMSSKAMAQD AAAGKTSFNK CLACHAIGEG AKNKVGPELN
     GLNGRKSGTA PDYSYSDANK NSGITWDEAT FKEYIKDPKA KIPGTKMAFA GIKNETEINN
     LWTFVSQFDK DGKIKQ