ID   CYAB_LEIDO              Reviewed;        1331 AA.
AC   Q25263;
DT   15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   03-AUG-2022, entry version 92.
DE   RecName: Full=Receptor-type adenylate cyclase B;
DE            EC=4.6.1.1;
DE   AltName: Full=ATP pyrophosphate-lyase;
DE   AltName: Full=Adenylyl cyclase;
GN   Name=RAC-B;
OS   Leishmania donovani.
OC   Eukaryota; Discoba; Euglenozoa; Kinetoplastea; Metakinetoplastina;
OC   Trypanosomatida; Trypanosomatidae; Leishmaniinae; Leishmania.
OX   NCBI_TaxID=5661;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=MHOM/SD/62/1S;
RX   PubMed=7615561; DOI=10.1074/jbc.270.29.17551;
RA   Sanchez M.A., Zeoli D., Klamo E.M., Kavanaugh M.P., Landfear S.M.;
RT   "A family of putative receptor-adenylate cyclases from Leishmania
RT   donovani.";
RL   J. Biol. Chem. 270:17551-17558(1995).
CC   -!- FUNCTION: Could act as a receptor for an unknown ligand.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP = 3',5'-cyclic AMP + diphosphate; Xref=Rhea:RHEA:15389,
CC         ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:58165; EC=4.6.1.1;
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC       Note=Binds 1 Mg(2+) ion per subunit. {ECO:0000250};
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000305}.
CC   -!- DEVELOPMENTAL STAGE: Expressed in the insect stage (promastigote) but
CC       not in the mammalian host stage of the parasite life cycle.
CC   -!- SIMILARITY: Belongs to the adenylyl cyclase class-3 family.
CC       {ECO:0000305}.
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DR   EMBL; U17043; AAA74999.1; -; Genomic_DNA.
DR   PIR; T18310; T18310.
DR   AlphaFoldDB; Q25263; -.
DR   SMR; Q25263; -.
DR   VEuPathDB; TriTrypDB:LdBPK_170140.1; -.
DR   VEuPathDB; TriTrypDB:LdBPK_363330.1; -.
DR   VEuPathDB; TriTrypDB:LdCL_170007400; -.
DR   VEuPathDB; TriTrypDB:LDHU3_17.0400; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0004016; F:adenylate cyclase activity; IEA:UniProtKB-EC.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0006171; P:cAMP biosynthetic process; IEA:UniProtKB-KW.
DR   GO; GO:0035556; P:intracellular signal transduction; IEA:InterPro.
DR   Gene3D; 3.30.70.1230; -; 1.
DR   InterPro; IPR001054; A/G_cyclase.
DR   InterPro; IPR029787; Nucleotide_cyclase.
DR   InterPro; IPR028082; Peripla_BP_I.
DR   Pfam; PF00211; Guanylate_cyc; 1.
DR   SMART; SM00044; CYCc; 1.
DR   SUPFAM; SSF53822; SSF53822; 1.
DR   SUPFAM; SSF55073; SSF55073; 1.
DR   PROSITE; PS50125; GUANYLATE_CYCLASE_2; 1.
PE   2: Evidence at transcript level;
KW   ATP-binding; cAMP biosynthesis; Glycoprotein; Lyase; Magnesium; Membrane;
KW   Metal-binding; Nucleotide-binding; Receptor; Transmembrane;
KW   Transmembrane helix.
FT   CHAIN           1..1331
FT                   /note="Receptor-type adenylate cyclase B"
FT                   /id="PRO_0000195735"
FT   TOPO_DOM        1..33
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        34..54
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        55..898
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        899..919
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        920..1331
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          940..1094
FT                   /note="Guanylate cyclase"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00099"
FT   BINDING         945
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000250"
FT   BINDING         988
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000250"
FT   CARBOHYD        255
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        429
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        558
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        574
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        657
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   1331 AA;  144163 MW;  5FC3AA22DC1E5072 CRC64;
     MYADATHPRR ACWCGAGGVS GCVRQRHAYR CSRLLAGVLL IVGALTLTLA VSTVPAAWAA
     GAVASSDEPV YLLNAMYSLS DYNAKHAKAL WLGIDSALHA VGYTAARGRP IKIIEPDPTD
     DLSDIVAVVL KALKDYPTLL GVIGPYSDTR LGAVLISPEI QNSGLMFLGP FTGSSSMRAW
     NENLYFMRAE PRLEIMAMVK HIANTFRARR TAFMYLTGEQ YGSFEHKSLV ELMTSLSLDP
     PAVYSASYST STAVNMTAFD AMADTRPQVI IIWGIPAGQV EELLKVVLTD PRTSSAYIMP
     SFALQQMTFQ VYYDLAMAGK LTPVDGQIIS SATSFPLTEP ASVHLRVFRA QMGEYMVKTG
     RVDASLWADE AKAVQQYGPW EHEASSSDSA AYVNNFFNEH PCVTQLMIAG WISGSLIAQT
     LAEENRIANR TAYRQYMFSQ QRYIVGEDFV LGDYGGPCNG VAEFLGAVCY CNQGGHSAVL
     SRLDRAVWTV ITESGVSFTQ KNCYSDGTTL PRPLNFLTLI FAEHPLLAQV GLTFKTSIST
     LVAYLQYNAS PVNAATVNVT DTTPQALHDA VTTNYTTDVV VGVTVKGMNV DGYLVPSPIH
     PRPHLVELLR NYVYLMPTLE QQMFVLYAKL SAVRGVTSID SAVHMILHGY ASDEVANITA
     VLRKSAATFN YDNPTVTAVP STKTVGSALA HGQINFVLAV TAADVADIVD FLVEEKTSIV
     VIVFDDLVIQ YPTLVTALKS KPASVQARVI TFTNLPLWSD TSESAHAASK LLTVFHDALP
     DPSQHTPGFL SAVLTGSFCA SMRRLADSVH STSLTDMVYR EGSVTTFAEP FGRFQWGCTT
     TPTDRFCVYH NYGAQGIVML SVQRMLDPTV PQLSSPMTPT MDYRPRQRSH ALTPAQRGGA
     IAGIALLTVI LLAVAGLALY CCMDNRNNDA APKDGDEPVT LLFTDIESST ALWAALPQLM
     SDAIAAHHRV IRQLVKKYGC YEVKTIGDSF MIACRSAHSA VSLACEIQTK LLKHDWGTEA
     LDRAYREFEL ARVDTLDDYE PPTARLSEEE YAALWCGLRV RVGIHTGLTD IRYDEVTRGY
     DYYGDTSNMA ARTEAVANGG QVVATEAAWW ALSNDERAGI AHTAMGPQGL RGVPFAVEMF
     QLNAVPGRRH AALRTEIEAI LPDDTATDTA SSAAGALLSS VGTINGPAAG IAFVLASCFA
     PYPVAQRVRE LQPLLSKWGV GAPPRSRLVS EEDYCQGLMN RLAIRIATVS QARCPVGNNG
     AAVDLDVQHA GTAEVMNPLL GEGSFISDGA RARHSGLTAV PPSAEPSAMR MRRVGRKVPE
     RPTVCNVRGA H