ID   CYAD_BORP1              Reviewed;         440 AA.
AC   J7QCA7; P11091;
DT   06-MAR-2013, integrated into UniProtKB/Swiss-Prot.
DT   31-OCT-2012, sequence version 1.
DT   25-MAY-2022, entry version 37.
DE   RecName: Full=Protein CyaD;
GN   Name=cyaD; OrderedLocusNames=BN118_0470;
OS   Bordetella pertussis (strain ATCC 9797 / DSM 5571 / NCTC 10739 / 18323).
OC   Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC   Alcaligenaceae; Bordetella.
OX   NCBI_TaxID=568706;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RC   STRAIN=ATCC 9797 / DSM 5571 / NCTC 10739 / 18323;
RX   PubMed=2905265; DOI=10.1002/j.1460-2075.1988.tb03288.x;
RA   Glaser P., Sakamoto H., Bellalou J., Ullmann A., Danchin A.;
RT   "Secretion of cyclolysin, the calmodulin-sensitive adenylate cyclase-
RT   haemolysin bifunctional protein of Bordetella pertussis.";
RL   EMBO J. 7:3997-4004(1988).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 9797 / DSM 5571 / NCTC 10739 / 18323;
RX   PubMed=23051057; DOI=10.1186/1471-2164-13-545;
RA   Park J., Zhang Y., Buboltz A.M., Zhang X., Schuster S.C., Ahuja U., Liu M.,
RA   Miller J.F., Sebaihia M., Bentley S.D., Parkhill J., Harvill E.T.;
RT   "Comparative genomics of the classical Bordetella subspecies: the evolution
RT   and exchange of virulence-associated diversity amongst closely related
RT   pathogens.";
RL   BMC Genomics 13:545-545(2012).
CC   -!- FUNCTION: CyaD is necessary for transport of calmodulin-sensitive
CC       adenylate cyclase-hemolysin (cyclolysin). {ECO:0000269|PubMed:2905265}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000305}; Single-pass
CC       membrane protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the membrane fusion protein (MFP) (TC 8.A.1)
CC       family. {ECO:0000305}.
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DR   EMBL; X14199; CAA32413.1; -; Genomic_DNA.
DR   EMBL; HE965805; CCJ61895.1; -; Genomic_DNA.
DR   PIR; S02387; BVBRCD.
DR   RefSeq; WP_010929997.1; NC_018518.1.
DR   AlphaFoldDB; J7QCA7; -.
DR   SMR; J7QCA7; -.
DR   STRING; 568706.BN118_0470; -.
DR   EnsemblBacteria; CCJ61895; CCJ61895; BN118_0470.
DR   GeneID; 45387799; -.
DR   KEGG; bper:BN118_0470; -.
DR   eggNOG; COG0845; Bacteria.
DR   HOGENOM; CLU_023976_0_1_4; -.
DR   OMA; EIKPGMP; -.
DR   Proteomes; UP000005250; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0044179; P:hemolysis in another organism; IEA:UniProtKB-KW.
DR   GO; GO:0009306; P:protein secretion; IEA:InterPro.
DR   InterPro; IPR039562; MFP_biotin_lipoyl_2.
DR   InterPro; IPR006144; Secretion_HlyD_CS.
DR   InterPro; IPR010129; T1SS_HlyD.
DR   Pfam; PF13533; Biotin_lipoyl_2; 1.
DR   TIGRFAMs; TIGR01843; type_I_hlyD; 1.
DR   PROSITE; PS00543; HLYD_FAMILY; 1.
PE   3: Inferred from homology;
KW   Cell inner membrane; Cell membrane; Cytolysis; Hemolysis; Membrane;
KW   Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..440
FT                   /note="Protein CyaD"
FT                   /id="PRO_0000421306"
FT   TOPO_DOM        1..55
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        56..75
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        76..440
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000255"
FT   CONFLICT        16
FT                   /note="L -> V (in Ref. 1; CAA32413)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        205..206
FT                   /note="EL -> DV (in Ref. 1; CAA32413)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   440 AA;  48054 MW;  27B773B66BD00FF3 CRC64;
     MRRALRELAA RHGRVLAASW RQRHRRPAGW FDPVETEFLP SALSLQERPI SPTARWLARI
     LMALAAGALV WSVVGKTEIV VHAAGKVVPV GQSKIIAASE TGRVARVLVA DNSRVAAGDV
     LLRLDAGVTE AEERKWRVQA AQARQDEARS RAMIRALDTG RAPVLAELPA DPGMMAAQSY
     LDSQYADYQA QLRSIEAAIA TYRRELGLVT QIAHAHRGLR RDGDVSQQAY LEKEQARMTL
     EGRLRQSEAQ RAALQTQTRR QAFETLVLAR KLAAQAEQEI ARTSAQRSRL VLTAPVDGVV
     QQLVALTEGT AVAATQPLMM VVPSGAGIQV QAQLDSKDIG FVRAGAPATV KVGAYDYTKY
     GTLEGKVLYV SPDTVVDDRQ QHSYRVTIAL AHPALEVDGK PRLLKEGMAV QADIRTGSRR
     LIEYLLSPVA RHAGESLGER