ID   CYAE_BORP1              Reviewed;         474 AA.
AC   J7RET5; P11092;
DT   06-MAR-2013, integrated into UniProtKB/Swiss-Prot.
DT   31-OCT-2012, sequence version 1.
DT   25-MAY-2022, entry version 35.
DE   RecName: Full=Protein CyaE;
DE   Flags: Precursor;
GN   Name=cyaE; OrderedLocusNames=BN118_0471;
OS   Bordetella pertussis (strain ATCC 9797 / DSM 5571 / NCTC 10739 / 18323).
OC   Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC   Alcaligenaceae; Bordetella.
OX   NCBI_TaxID=568706;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RC   STRAIN=ATCC 9797 / DSM 5571 / NCTC 10739 / 18323;
RX   PubMed=2905265; DOI=10.1002/j.1460-2075.1988.tb03288.x;
RA   Glaser P., Sakamoto H., Bellalou J., Ullmann A., Danchin A.;
RT   "Secretion of cyclolysin, the calmodulin-sensitive adenylate cyclase-
RT   haemolysin bifunctional protein of Bordetella pertussis.";
RL   EMBO J. 7:3997-4004(1988).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 9797 / DSM 5571 / NCTC 10739 / 18323;
RX   PubMed=23051057; DOI=10.1186/1471-2164-13-545;
RA   Park J., Zhang Y., Buboltz A.M., Zhang X., Schuster S.C., Ahuja U., Liu M.,
RA   Miller J.F., Sebaihia M., Bentley S.D., Parkhill J., Harvill E.T.;
RT   "Comparative genomics of the classical Bordetella subspecies: the evolution
RT   and exchange of virulence-associated diversity amongst closely related
RT   pathogens.";
RL   BMC Genomics 13:545-545(2012).
CC   -!- FUNCTION: CyaE is necessary for transport of calmodulin-sensitive
CC       adenylate cyclase-hemolysin (cyclolysin). {ECO:0000269|PubMed:2905265}.
CC   -!- SUBCELLULAR LOCATION: Cell outer membrane {ECO:0000250}; Peripheral
CC       membrane protein {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the outer membrane factor (OMF) (TC 1.B.17)
CC       family. {ECO:0000305}.
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DR   EMBL; X14199; CAA32414.1; -; Genomic_DNA.
DR   EMBL; HE965805; CCJ61896.1; -; Genomic_DNA.
DR   PIR; S02388; BVBRCE.
DR   RefSeq; WP_010929998.1; NC_018518.1.
DR   AlphaFoldDB; J7RET5; -.
DR   SMR; J7RET5; -.
DR   STRING; 568706.BN118_0471; -.
DR   EnsemblBacteria; CCJ61896; CCJ61896; BN118_0471.
DR   GeneID; 45387800; -.
DR   KEGG; bper:BN118_0471; -.
DR   eggNOG; COG1538; Bacteria.
DR   HOGENOM; CLU_012817_10_2_4; -.
DR   OMA; SQTIWDS; -.
DR   Proteomes; UP000005250; Chromosome.
DR   GO; GO:0009279; C:cell outer membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0015562; F:efflux transmembrane transporter activity; IEA:InterPro.
DR   GO; GO:0044179; P:hemolysis in another organism; IEA:UniProtKB-KW.
DR   InterPro; IPR028351; CyaE.
DR   InterPro; IPR003423; OMP_efflux.
DR   Pfam; PF02321; OEP; 2.
DR   PIRSF; PIRSF001892; CyaE; 1.
PE   3: Inferred from homology;
KW   Cell outer membrane; Cytolysis; Hemolysis; Membrane; Signal; Transmembrane;
KW   Transmembrane beta strand; Transport.
FT   SIGNAL          1..31
FT                   /evidence="ECO:0000255"
FT   CHAIN           32..474
FT                   /note="Protein CyaE"
FT                   /id="PRO_0000421307"
SQ   SEQUENCE   474 AA;  50205 MW;  29A4F21D377FC957 CRC64;
     MAAVQVRRRG RALALALWAG FALSVGGGVR ARDGLATPPA FEGQAAPAVS WPCPPPADRL
     DDLALLEAID LALCHSPALR QGWARIKARS AEVGLARAPY YPSVALSAGR SAQRRSTGLG
     EDGVRNNVMA VTLAWRLFDS GARSAALRAA QAQLDEAAQA YGAVLQDKLA EVVGAYYEAA
     TARQALHTAV EDTEIARRSA SIAARRARAG LDSHGDVLHA QAALERARLA QAQAEGAQAR
     ALAGLAQVLG VDPATPIVLA PGPLAPQRIE DRELAQWLRD ARQRHPAIKA AQAGLAAATA
     QVDVARATDM PTVDLSLGHY RNSSENVSVF AGSSRSVSAS LNLRIPLFDG FARRHRIQGA
     RAEVQRQEAL LDQARLATGA AVARAYADLR AARASHEASL RWLKAARAAY ESDLRRYEAG
     VGGVAELLRA QSDWLSARQR HVLYAAQLRT RALALLAAAG ELGRSTIDGD PPKE