ID   CYAY_ECO57              Reviewed;         106 AA.
AC   Q8XAP0;
DT   27-MAR-2002, integrated into UniProtKB/Swiss-Prot.
DT   27-MAR-2002, sequence version 1.
DT   03-AUG-2022, entry version 110.
DE   RecName: Full=Iron-sulfur cluster assembly protein CyaY {ECO:0000255|HAMAP-Rule:MF_00142};
GN   Name=cyaY {ECO:0000255|HAMAP-Rule:MF_00142};
GN   OrderedLocusNames=Z5323, ECs4738;
OS   Escherichia coli O157:H7.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83334;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=O157:H7 / EDL933 / ATCC 700927 / EHEC;
RX   PubMed=11206551; DOI=10.1038/35054089;
RA   Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J.,
RA   Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G.,
RA   Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J.,
RA   Davis N.W., Lim A., Dimalanta E.T., Potamousis K., Apodaca J.,
RA   Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A.,
RA   Blattner F.R.;
RT   "Genome sequence of enterohaemorrhagic Escherichia coli O157:H7.";
RL   Nature 409:529-533(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=O157:H7 / Sakai / RIMD 0509952 / EHEC;
RX   PubMed=11258796; DOI=10.1093/dnares/8.1.11;
RA   Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K.,
RA   Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T.,
RA   Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T., Kuhara S.,
RA   Shiba T., Hattori M., Shinagawa H.;
RT   "Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and
RT   genomic comparison with a laboratory strain K-12.";
RL   DNA Res. 8:11-22(2001).
RN   [3]
RP   INTERACTION WITH ISCS AND ISCU, AND SUBUNIT.
RC   STRAIN=O157:H7 / EDL933 / ATCC 700927 / EHEC;
RX   PubMed=20404999; DOI=10.1371/journal.pbio.1000354;
RA   Shi R., Proteau A., Villarroya M., Moukadiri I., Zhang L., Trempe J.F.,
RA   Matte A., Armengod M.E., Cygler M.;
RT   "Structural basis for Fe-S cluster assembly and tRNA thiolation mediated by
RT   IscS protein-protein interactions.";
RL   PLoS Biol. 8:E1000354-E1000354(2010).
CC   -!- FUNCTION: Involved in iron-sulfur (Fe-S) cluster assembly. May act as a
CC       regulator of Fe-S biogenesis. {ECO:0000255|HAMAP-Rule:MF_00142}.
CC   -!- SUBUNIT: Interacts with IscS. Certain pairs of proteins can bind
CC       simultaneously to IscS; IscS-IscU-CyaY complexes can be isolated in
CC       vitro, but (IscS-TusA-CyaY) complexes cannot.
CC       {ECO:0000269|PubMed:20404999}.
CC   -!- INTERACTION:
CC       Q8XAP0; P0A6B9: iscS; NbExp=2; IntAct=EBI-15850020, EBI-9011195;
CC   -!- SIMILARITY: Belongs to the frataxin family. {ECO:0000255|HAMAP-
CC       Rule:MF_00142}.
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DR   EMBL; AE005174; AAG58999.1; -; Genomic_DNA.
DR   EMBL; BA000007; BAB38160.1; -; Genomic_DNA.
DR   PIR; A91221; A91221.
DR   PIR; C86067; C86067.
DR   RefSeq; NP_312764.1; NC_002695.1.
DR   RefSeq; WP_000999944.1; NZ_SWKA01000005.1.
DR   AlphaFoldDB; Q8XAP0; -.
DR   SMR; Q8XAP0; -.
DR   DIP; DIP-58577N; -.
DR   IntAct; Q8XAP0; 2.
DR   STRING; 155864.EDL933_5125; -.
DR   EnsemblBacteria; AAG58999; AAG58999; Z5323.
DR   EnsemblBacteria; BAB38160; BAB38160; ECs_4738.
DR   GeneID; 915190; -.
DR   KEGG; ece:Z5323; -.
DR   KEGG; ecs:ECs_4738; -.
DR   PATRIC; fig|386585.9.peg.4943; -.
DR   eggNOG; COG1965; Bacteria.
DR   HOGENOM; CLU_080880_3_0_6; -.
DR   OMA; EPMHEIW; -.
DR   Proteomes; UP000000558; Chromosome.
DR   Proteomes; UP000002519; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProt.
DR   GO; GO:0008199; F:ferric iron binding; IEA:InterPro.
DR   GO; GO:0016226; P:iron-sulfur cluster assembly; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.30.920.10; -; 1.
DR   HAMAP; MF_00142; CyaY; 1.
DR   InterPro; IPR002908; Frataxin/CyaY.
DR   InterPro; IPR036524; Frataxin/CyaY_sf.
DR   InterPro; IPR020895; Frataxin_CS.
DR   PANTHER; PTHR16821; PTHR16821; 1.
DR   Pfam; PF01491; Frataxin_Cyay; 1.
DR   SMART; SM01219; Frataxin_Cyay; 1.
DR   SUPFAM; SSF55387; SSF55387; 1.
DR   TIGRFAMs; TIGR03421; FeS_CyaY; 1.
DR   PROSITE; PS01344; FRATAXIN_1; 1.
DR   PROSITE; PS50810; FRATAXIN_2; 1.
PE   1: Evidence at protein level;
KW   Iron; Metal-binding; Reference proteome.
FT   CHAIN           1..106
FT                   /note="Iron-sulfur cluster assembly protein CyaY"
FT                   /id="PRO_0000193938"
SQ   SEQUENCE   106 AA;  12201 MW;  03EA6985BA48F711 CRC64;
     MNDSEFHRLA DQLWLTIEER LDDWDGDSDI DCEINGGVLT ITFENGSKII INRQEPLHQV
     WLATKQGGYH FDLKGDEWIC DRSGETFRDL LEQAATQQAG ETVSFR