CYB5R_DROME
ID CYB5R_DROME Reviewed; 436 AA.
AC P19967; Q9VJI2;
DT 01-FEB-1991, integrated into UniProtKB/Swiss-Prot.
DT 21-JUN-2005, sequence version 2.
DT 03-AUG-2022, entry version 158.
DE RecName: Full=Cytochrome b5-related protein;
DE AltName: Full=Protein TU-36B;
GN Name=Cyt-b5-r; ORFNames=CG13279;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=2549511; DOI=10.1093/nar/17.15.6349;
RA Levin R.J., Boychuk P.L., Croniger C.M., Kazzaz J.A., Rozek C.E.;
RT "Structure and expression of a muscle specific gene which is adjacent to
RT the Drosophila myosin heavy-chain gene and can encode a cytochrome b
RT related protein.";
RL Nucleic Acids Res. 17:6349-6367(1989).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Head;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
CC -!- FUNCTION: May play a role in muscle cell metabolism.
CC -!- TISSUE SPECIFICITY: Muscle.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAA33113.1; Type=Frameshift; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; X15008; CAA33113.1; ALT_FRAME; Genomic_DNA.
DR EMBL; AE014134; AAF53567.1; -; Genomic_DNA.
DR EMBL; AY058287; AAL13516.1; -; mRNA.
DR PIR; S05441; S05441.
DR RefSeq; NP_001260514.1; NM_001273585.1.
DR RefSeq; NP_477154.1; NM_057806.5.
DR AlphaFoldDB; P19967; -.
DR SMR; P19967; -.
DR BioGRID; 61015; 4.
DR IntAct; P19967; 1.
DR STRING; 7227.FBpp0304264; -.
DR PaxDb; P19967; -.
DR DNASU; 35008; -.
DR EnsemblMetazoa; FBtr0080932; FBpp0080486; FBgn0000406.
DR EnsemblMetazoa; FBtr0331931; FBpp0304264; FBgn0000406.
DR GeneID; 35008; -.
DR KEGG; dme:Dmel_CG13279; -.
DR CTD; 35008; -.
DR FlyBase; FBgn0000406; Cyt-b5-r.
DR VEuPathDB; VectorBase:FBgn0000406; -.
DR eggNOG; KOG4232; Eukaryota.
DR GeneTree; ENSGT00940000176788; -.
DR HOGENOM; CLU_627470_0_0_1; -.
DR InParanoid; P19967; -.
DR OMA; GPFWIRE; -.
DR OrthoDB; 1060606at2759; -.
DR PhylomeDB; P19967; -.
DR SignaLink; P19967; -.
DR BioGRID-ORCS; 35008; 0 hits in 1 CRISPR screen.
DR ChiTaRS; Cyt-b5-r; fly.
DR GenomeRNAi; 35008; -.
DR PRO; PR:P19967; -.
DR Proteomes; UP000000803; Chromosome 2L.
DR Bgee; FBgn0000406; Expressed in head capsule and 34 other tissues.
DR ExpressionAtlas; P19967; baseline and differential.
DR Genevisible; P19967; DM.
DR GO; GO:0005739; C:mitochondrion; IDA:FlyBase.
DR GO; GO:0020037; F:heme binding; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0016491; F:oxidoreductase activity; IEA:InterPro.
DR GO; GO:0006629; P:lipid metabolic process; IEA:InterPro.
DR Gene3D; 3.10.120.10; -; 1.
DR InterPro; IPR001199; Cyt_B5-like_heme/steroid-bd.
DR InterPro; IPR036400; Cyt_B5-like_heme/steroid_sf.
DR InterPro; IPR018506; Cyt_B5_heme-BS.
DR InterPro; IPR005804; FA_desaturase_dom.
DR InterPro; IPR012171; Fatty_acid_desaturase.
DR Pfam; PF00173; Cyt-b5; 1.
DR Pfam; PF00487; FA_desaturase; 1.
DR PIRSF; PIRSF015921; FA_sphinglp_des; 1.
DR SMART; SM01117; Cyt-b5; 1.
DR SUPFAM; SSF55856; SSF55856; 1.
DR PROSITE; PS00191; CYTOCHROME_B5_1; 1.
DR PROSITE; PS50255; CYTOCHROME_B5_2; 1.
PE 2: Evidence at transcript level;
KW Heme; Iron; Metal-binding; Reference proteome.
FT CHAIN 1..436
FT /note="Cytochrome b5-related protein"
FT /id="PRO_0000166018"
FT DOMAIN 16..100
FT /note="Cytochrome b5 heme-binding"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00279"
FT BINDING 59
FT /ligand="heme"
FT /ligand_id="ChEBI:CHEBI:30413"
FT /ligand_part="Fe"
FT /ligand_part_id="ChEBI:CHEBI:18248"
FT /note="axial binding residue"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00279"
FT BINDING 82
FT /ligand="heme"
FT /ligand_id="ChEBI:CHEBI:30413"
FT /ligand_part="Fe"
FT /ligand_part_id="ChEBI:CHEBI:18248"
FT /note="axial binding residue"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00279"
FT VARIANT 202
FT /note="A -> P"
FT VARIANT 213
FT /note="I -> T"
FT VARIANT 344
FT /note="R -> G"
FT CONFLICT 219..220
FT /note="FD -> LN (in Ref. 1; CAA33113)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 436 AA; 50355 MW; 77C77E49C6218A02 CRC64;
MVIEEWKKSG IATKFPTYRN SALITTHSWQ KGKRQDDGAE GLWRINDGIY DFTSFIDKHP
GGPFWIRETK GTDITEAFEA HHLTTAPEKM IAKYKVRDAA EPRIYTLTLE EGGFYKTLKE
RVREQLKTID KRPKKKSDLI HLGLVVSLYL LGIASAKYNS LLALVLASVA LCWTVIVSHN
YFHRRDNWQM YAFNLGMMNF AAWRVSHALS HHIYPNSYFD LELSMFEPLL CWVPNPHIKS
KLMRYVSWVT EPVAYALAFF IQMGTRIFYS LRHTNILYWH DLLPLTIPIA IYLGTGGSLG
IWICVRQWLA MTSIASFSFC LIGLNAAHHD PEIYHEGDAN REDRDWGLFQ VDTIIDRGDL
KWSQFLVLTH FGDHVLHHLF PTLDHGLLPA LYPVLYQTLD EFKGHLRECN HIEHMIGQHK
QLLRIEPNPR APGAGK
