CYB5_CHICK
ID CYB5_CHICK Reviewed; 138 AA.
AC P00174;
DT 21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1990, sequence version 4.
DT 03-AUG-2022, entry version 159.
DE RecName: Full=Cytochrome b5;
GN Name=CYB5A; Synonyms=CYB5;
OS Gallus gallus (Chicken).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Archelosauria; Archosauria; Dinosauria; Saurischia; Theropoda;
OC Coelurosauria; Aves; Neognathae; Galloanserae; Galliformes; Phasianidae;
OC Phasianinae; Gallus.
OX NCBI_TaxID=9031;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=2369133; DOI=10.1016/0003-9861(90)90350-8;
RA Zhang H., Somerville C.;
RT "Soluble and membrane-bound forms of cytochrome b5 are the products of a
RT single gene in chicken.";
RL Arch. Biochem. Biophys. 280:412-415(1990).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=3395128; DOI=10.1016/0003-9861(88)90603-0;
RA Zhang H., Somerville C.;
RT "The primary structure of chicken liver cytochrome b5 deduced from the DNA
RT sequence of a cDNA clone.";
RL Arch. Biochem. Biophys. 264:343-347(1988).
RN [3]
RP PROTEIN SEQUENCE OF 14-97.
RX PubMed=4993957; DOI=10.1016/s0021-9258(18)62368-3;
RA Nobrega F.G., Ozols J.;
RT "Amino acid sequences of tryptic peptides of cytochromes b5 from microsomes
RT of human, monkey, porcine, and chicken liver.";
RL J. Biol. Chem. 246:1706-1717(1971).
RN [4]
RP PROTEIN SEQUENCE OF 4-16 AND 89-138.
RX PubMed=2752049; DOI=10.1016/0167-4838(89)90143-x;
RA Ozols J.;
RT "Structure of cytochrome b5 and its topology in the microsomal membrane.";
RL Biochim. Biophys. Acta 997:121-130(1989).
CC -!- FUNCTION: Cytochrome b5 is a membrane-bound hemoprotein functioning as
CC an electron carrier for several membrane-bound oxygenases.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Single-pass
CC membrane protein; Cytoplasmic side. Microsome membrane; Single-pass
CC membrane protein; Cytoplasmic side.
CC -!- SIMILARITY: Belongs to the cytochrome b5 family. {ECO:0000305}.
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DR EMBL; M32293; AAA48740.1; -; mRNA.
DR EMBL; M18539; AAA48733.1; -; mRNA.
DR PIR; A28811; CBCH5.
DR RefSeq; NP_001001748.1; NM_001001748.2.
DR AlphaFoldDB; P00174; -.
DR SMR; P00174; -.
DR STRING; 9031.ENSGALP00000022250; -.
DR PaxDb; P00174; -.
DR Ensembl; ENSGALT00000022289; ENSGALP00000022250; ENSGALG00000013708.
DR GeneID; 414798; -.
DR KEGG; gga:414798; -.
DR CTD; 1528; -.
DR VEuPathDB; HostDB:geneid_414798; -.
DR eggNOG; KOG0537; Eukaryota.
DR GeneTree; ENSGT00940000156770; -.
DR HOGENOM; CLU_102602_3_3_1; -.
DR InParanoid; P00174; -.
DR OMA; RHKIAKP; -.
DR OrthoDB; 1566561at2759; -.
DR PhylomeDB; P00174; -.
DR TreeFam; TF314537; -.
DR Reactome; R-GGA-196836; Vitamin C (ascorbate) metabolism.
DR Reactome; R-GGA-9609523; Insertion of tail-anchored proteins into the endoplasmic reticulum membrane.
DR PRO; PR:P00174; -.
DR Proteomes; UP000000539; Chromosome 2.
DR Bgee; ENSGALG00000013708; Expressed in kidney and 14 other tissues.
DR ExpressionAtlas; P00174; baseline and differential.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IBA:GO_Central.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0043231; C:intracellular membrane-bounded organelle; IBA:GO_Central.
DR GO; GO:0016020; C:membrane; IBA:GO_Central.
DR GO; GO:0020037; F:heme binding; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR Gene3D; 3.10.120.10; -; 1.
DR InterPro; IPR001199; Cyt_B5-like_heme/steroid-bd.
DR InterPro; IPR036400; Cyt_B5-like_heme/steroid_sf.
DR InterPro; IPR018506; Cyt_B5_heme-BS.
DR Pfam; PF00173; Cyt-b5; 1.
DR PRINTS; PR00363; CYTOCHROMEB5.
DR SMART; SM01117; Cyt-b5; 1.
DR SUPFAM; SSF55856; SSF55856; 1.
DR PROSITE; PS00191; CYTOCHROME_B5_1; 1.
DR PROSITE; PS50255; CYTOCHROME_B5_2; 1.
PE 1: Evidence at protein level;
KW Direct protein sequencing; Electron transport; Endoplasmic reticulum; Heme;
KW Iron; Membrane; Metal-binding; Microsome; Reference proteome;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..138
FT /note="Cytochrome b5"
FT /id="PRO_0000166015"
FT TRANSMEM 114..136
FT /note="Helical"
FT /evidence="ECO:0000255"
FT DOMAIN 14..90
FT /note="Cytochrome b5 heme-binding"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00279"
FT BINDING 49
FT /ligand="heme"
FT /ligand_id="ChEBI:CHEBI:30413"
FT /ligand_part="Fe"
FT /ligand_part_id="ChEBI:CHEBI:18248"
FT /note="axial binding residue"
FT BINDING 73
FT /ligand="heme"
FT /ligand_id="ChEBI:CHEBI:30413"
FT /ligand_part="Fe"
FT /ligand_part_id="ChEBI:CHEBI:18248"
FT /note="axial binding residue"
FT CONFLICT 12
FT /note="W -> E (in Ref. 4; AA sequence)"
FT /evidence="ECO:0000305"
FT CONFLICT 27..29
FT /note="NSQ -> ZSB (in Ref. 3; AA sequence)"
FT /evidence="ECO:0000305"
FT CONFLICT 67
FT /note="N -> D (in Ref. 3; AA sequence)"
FT /evidence="ECO:0000305"
FT CONFLICT 124
FT /note="A -> T (in Ref. 4; AA sequence)"
FT /evidence="ECO:0000305"
FT CONFLICT 138
FT /note="E -> EE (in Ref. 4; AA sequence)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 138 AA; 15545 MW; 168F0B87251557A8 CRC64;
MVGSSEAGGE AWRGRYYRLE EVQKHNNSQS TWIIVHHRIY DITKFLDEHP GGEEVLREQA
GGDATENFED VGHSTDARAL SETFIIGELH PDDRPKLQKP AETLITTVQS NSSSWSNWVI
PAIAAIIVAL MYRSYMSE
