CYB_CAEBR
ID CYB_CAEBR Reviewed; 370 AA.
AC Q8HEC2; B1PE38; B1PE50; B1PE62; B1PED4; B1PEF8; B1PEI2; B1PEK6; B1PEP2;
AC B1PEU0;
DT 21-JUN-2005, integrated into UniProtKB/Swiss-Prot.
DT 13-OCT-2009, sequence version 3.
DT 03-AUG-2022, entry version 88.
DE RecName: Full=Cytochrome b;
DE AltName: Full=Complex III subunit 3;
DE AltName: Full=Complex III subunit III;
DE AltName: Full=Cytochrome b-c1 complex subunit 3;
DE AltName: Full=Ubiquinol-cytochrome-c reductase complex cytochrome b subunit;
GN Name=ctb-1; Synonyms=cob, cytb, Mtcyb;
OS Caenorhabditis briggsae.
OG Mitochondrion.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6238;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND VARIANTS THR-97; LEU-321 AND
RP PHE-342.
RC STRAIN=PB800;
RX PubMed=12644560; DOI=10.1093/molbev/msg044;
RA Denver D.R., Morris K., Thomas W.K.;
RT "Phylogenetics in Caenorhabditis elegans: an analysis of divergence and
RT outcrossing.";
RL Mol. Biol. Evol. 20:393-400(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND VARIANTS SER-54; THR-97; LEU-142;
RP ILE-205; THR-222; PHE-234; LEU-257; VAL-297; TRP-317; LEU-321; PHE-342;
RP ILE-342 AND VAL-353.
RC STRAIN=BW287, ED3032, ED3033, ED3034, ED3035, ED3036, ED3037, ED3083,
RC ED3092, ED3101, EG4181, EG4207A, HK104, HK105, JU403, JU439, JU516, JU725,
RC JU726, JU793, PB800, PB826, and VT847;
RX PubMed=18302772; DOI=10.1186/1471-2148-8-62;
RA Howe D.K., Denver D.R.;
RT "Muller's Ratchet and compensatory mutation in Caenorhabditis briggsae
RT mitochondrial genome evolution.";
RL BMC Evol. Biol. 8:62-62(2008).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AF16;
RX PubMed=14624247; DOI=10.1371/journal.pbio.0000045;
RA Stein L.D., Bao Z., Blasiar D., Blumenthal T., Brent M.R., Chen N.,
RA Chinwalla A., Clarke L., Clee C., Coghlan A., Coulson A., D'Eustachio P.,
RA Fitch D.H.A., Fulton L.A., Fulton R.E., Griffiths-Jones S., Harris T.W.,
RA Hillier L.W., Kamath R., Kuwabara P.E., Mardis E.R., Marra M.A.,
RA Miner T.L., Minx P., Mullikin J.C., Plumb R.W., Rogers J., Schein J.E.,
RA Sohrmann M., Spieth J., Stajich J.E., Wei C., Willey D., Wilson R.K.,
RA Durbin R.M., Waterston R.H.;
RT "The genome sequence of Caenorhabditis briggsae: a platform for comparative
RT genomics.";
RL PLoS Biol. 1:166-192(2003).
CC -!- FUNCTION: Component of the ubiquinol-cytochrome c reductase complex
CC (complex III or cytochrome b-c1 complex) that is part of the
CC mitochondrial respiratory chain. The b-c1 complex mediates electron
CC transfer from ubiquinol to cytochrome c. Contributes to the generation
CC of a proton gradient across the mitochondrial membrane that is then
CC used for ATP synthesis. {ECO:0000250|UniProtKB:P00163}.
CC -!- COFACTOR:
CC Name=heme b; Xref=ChEBI:CHEBI:60344;
CC Evidence={ECO:0000250|UniProtKB:P00163};
CC Note=Binds 2 heme b groups non-covalently.
CC {ECO:0000250|UniProtKB:P00163};
CC -!- SUBUNIT: The main subunits of complex b-c1 are: cytochrome b,
CC cytochrome c1 and the Rieske protein. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane
CC {ECO:0000250|UniProtKB:P00163}; Multi-pass membrane protein
CC {ECO:0000250|UniProtKB:P00163}.
CC -!- MISCELLANEOUS: Heme 1 (or BL or b562) is low-potential and absorbs at
CC about 562 nm, and heme 2 (or BH or b566) is high-potential and absorbs
CC at about 566 nm. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the cytochrome b family. {ECO:0000255|PROSITE-
CC ProRule:PRU00967, ECO:0000255|PROSITE-ProRule:PRU00968}.
CC -!- CAUTION: The protein contains an even number of transmembrane helices,
CC fewer than predicted by bioinformatics tools.
CC {ECO:0000250|UniProtKB:P00163}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAO13511.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AY171104; AAO13511.1; ALT_INIT; Genomic_DNA.
DR EMBL; EU407781; ACB06105.1; -; Genomic_DNA.
DR EMBL; EU407782; ACB06117.1; -; Genomic_DNA.
DR EMBL; EU407783; ACB06129.1; -; Genomic_DNA.
DR EMBL; EU407784; ACB06141.1; -; Genomic_DNA.
DR EMBL; EU407785; ACB06153.1; -; Genomic_DNA.
DR EMBL; EU407786; ACB06165.1; -; Genomic_DNA.
DR EMBL; EU407787; ACB06177.1; -; Genomic_DNA.
DR EMBL; EU407788; ACB06189.1; -; Genomic_DNA.
DR EMBL; EU407789; ACB06201.1; -; Genomic_DNA.
DR EMBL; EU407790; ACB06213.1; -; Genomic_DNA.
DR EMBL; EU407791; ACB06225.1; -; Genomic_DNA.
DR EMBL; EU407792; ACB06237.1; -; Genomic_DNA.
DR EMBL; EU407793; ACB06249.1; -; Genomic_DNA.
DR EMBL; EU407794; ACB06261.1; -; Genomic_DNA.
DR EMBL; EU407795; ACB06273.1; -; Genomic_DNA.
DR EMBL; EU407796; ACB06285.1; -; Genomic_DNA.
DR EMBL; EU407797; ACB06297.1; -; Genomic_DNA.
DR EMBL; EU407798; ACB06309.1; -; Genomic_DNA.
DR EMBL; EU407799; ACB06321.1; -; Genomic_DNA.
DR EMBL; EU407800; ACB06333.1; -; Genomic_DNA.
DR EMBL; EU407801; ACB06345.1; -; Genomic_DNA.
DR EMBL; EU407802; ACB06357.1; -; Genomic_DNA.
DR EMBL; EU407803; ACB06369.1; -; Genomic_DNA.
DR EMBL; AC186293; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR RefSeq; YP_001552233.1; NC_009885.1.
DR AlphaFoldDB; Q8HEC2; -.
DR SMR; Q8HEC2; -.
DR GeneID; 5666637; -.
DR KEGG; cbr:CYTB; -.
DR CTD; 4519; -.
DR InParanoid; Q8HEC2; -.
DR Proteomes; UP000008549; Mitochondrion.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005743; C:mitochondrial inner membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0070469; C:respirasome; IEA:UniProtKB-KW.
DR GO; GO:0009055; F:electron transfer activity; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0022904; P:respiratory electron transport chain; IEA:InterPro.
DR CDD; cd00290; cytochrome_b_C; 1.
DR CDD; cd00284; Cytochrome_b_N; 1.
DR Gene3D; 1.20.810.10; -; 1.
DR InterPro; IPR005798; Cyt_b/b6_C.
DR InterPro; IPR036150; Cyt_b/b6_C_sf.
DR InterPro; IPR005797; Cyt_b/b6_N.
DR InterPro; IPR027387; Cytb/b6-like_sf.
DR InterPro; IPR016174; Di-haem_cyt_TM.
DR Pfam; PF00032; Cytochrom_B_C; 1.
DR Pfam; PF00033; Cytochrome_B; 1.
DR SUPFAM; SSF81342; SSF81342; 1.
DR SUPFAM; SSF81648; SSF81648; 1.
DR PROSITE; PS51003; CYTB_CTER; 1.
DR PROSITE; PS51002; CYTB_NTER; 1.
PE 3: Inferred from homology;
KW Electron transport; Heme; Iron; Membrane; Metal-binding; Mitochondrion;
KW Mitochondrion inner membrane; Reference proteome; Respiratory chain;
KW Transmembrane; Transmembrane helix; Transport; Ubiquinone.
FT CHAIN 1..370
FT /note="Cytochrome b"
FT /id="PRO_0000060702"
FT TRANSMEM 30..50
FT /note="Helical"
FT /evidence="ECO:0000250|UniProtKB:P00163"
FT TRANSMEM 74..96
FT /note="Helical"
FT /evidence="ECO:0000250|UniProtKB:P00163"
FT TRANSMEM 109..129
FT /note="Helical"
FT /evidence="ECO:0000250|UniProtKB:P00163"
FT TRANSMEM 175..195
FT /note="Helical"
FT /evidence="ECO:0000250|UniProtKB:P00163"
FT TRANSMEM 221..240
FT /note="Helical"
FT /evidence="ECO:0000250|UniProtKB:P00163"
FT TRANSMEM 284..304
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 316..336
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 342..362
FT /note="Helical"
FT /evidence="ECO:0000255"
FT BINDING 80
FT /ligand="heme b"
FT /ligand_id="ChEBI:CHEBI:60344"
FT /ligand_label="b562"
FT /ligand_part="Fe"
FT /ligand_part_id="ChEBI:CHEBI:18248"
FT /note="axial binding residue"
FT /evidence="ECO:0000250|UniProtKB:P00163"
FT BINDING 94
FT /ligand="heme b"
FT /ligand_id="ChEBI:CHEBI:60344"
FT /ligand_label="b566"
FT /ligand_part="Fe"
FT /ligand_part_id="ChEBI:CHEBI:18248"
FT /note="axial binding residue"
FT /evidence="ECO:0000250|UniProtKB:P00163"
FT BINDING 179
FT /ligand="heme b"
FT /ligand_id="ChEBI:CHEBI:60344"
FT /ligand_label="b562"
FT /ligand_part="Fe"
FT /ligand_part_id="ChEBI:CHEBI:18248"
FT /note="axial binding residue"
FT /evidence="ECO:0000250|UniProtKB:P00163"
FT BINDING 193
FT /ligand="heme b"
FT /ligand_id="ChEBI:CHEBI:60344"
FT /ligand_label="b566"
FT /ligand_part="Fe"
FT /ligand_part_id="ChEBI:CHEBI:18248"
FT /note="axial binding residue"
FT /evidence="ECO:0000250|UniProtKB:P00163"
FT BINDING 198
FT /ligand="a ubiquinone"
FT /ligand_id="ChEBI:CHEBI:16389"
FT /evidence="ECO:0000250|UniProtKB:P00157"
FT VARIANT 54
FT /note="P -> S (in strain: JU403, JU439, JU516 and JU793)"
FT /evidence="ECO:0000269|PubMed:18302772"
FT VARIANT 97
FT /note="K -> T (in strain: PB800)"
FT /evidence="ECO:0000269|PubMed:12644560,
FT ECO:0000269|PubMed:18302772"
FT VARIANT 142
FT /note="V -> L (in strain: EG4181)"
FT /evidence="ECO:0000269|PubMed:18302772"
FT VARIANT 205
FT /note="S -> I (in strain: JU403, JU439, JU516 and JU793)"
FT /evidence="ECO:0000269|PubMed:18302772"
FT VARIANT 222
FT /note="I -> T (in strain: ED3092 and ED3101)"
FT /evidence="ECO:0000269|PubMed:18302772"
FT VARIANT 234
FT /note="V -> F (in strain: ED3092 and ED3101)"
FT /evidence="ECO:0000269|PubMed:18302772"
FT VARIANT 257
FT /note="P -> L (in strain: HK104)"
FT /evidence="ECO:0000269|PubMed:18302772"
FT VARIANT 297
FT /note="F -> V (in strain: JU725)"
FT /evidence="ECO:0000269|PubMed:18302772"
FT VARIANT 317
FT /note="L -> W (in strain: JU725)"
FT /evidence="ECO:0000269|PubMed:18302772"
FT VARIANT 321
FT /note="F -> L (in strain: PB800)"
FT /evidence="ECO:0000269|PubMed:12644560,
FT ECO:0000269|PubMed:18302772"
FT VARIANT 342
FT /note="V -> F (in strain: BW287, EG4181, EG4207A, HK104,
FT HK105, JU403, JU439, JU516, JU725, JU793, PB800 and PB826)"
FT /evidence="ECO:0000269|PubMed:12644560,
FT ECO:0000269|PubMed:18302772"
FT VARIANT 342
FT /note="V -> I (in strain: ED3033, ED3034, ED3035, ED3037,
FT ED3092, ED3101 and VT847)"
FT /evidence="ECO:0000269|PubMed:18302772"
FT VARIANT 353
FT /note="G -> V (in strain: PB826)"
FT /evidence="ECO:0000269|PubMed:18302772"
FT CONFLICT 188
FT /note="F -> V (in Ref. 1; AAO13511 and 2; ACB06105/
FT ACB06117/ACB06129/ACB06141/ACB06153/ACB06165/ACB06177/
FT ACB06189/ACB06201/ACB06213/ACB06225/ACB06237/ACB06249/
FT ACB06261/ACB06273/ACB06285/ACB06297/ACB06309/ACB06321/
FT ACB06333/ACB06345/ACB06357/ACB06369)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 370 AA; 42442 MW; 998F4A83D21B036F CRC64;
MKINNSLLNF VNGMLVTLPS SKTLTLSWNF GSMLGMVLVF QIVTGTFLAF YYTPDSLMAF
STVQYIMYEV NFGWIFRIFH FNGASLFFIF LYLHIFKGLF FMSYRLKKVW MSGLTIYLLV
MMEAFMGYVL VWAQMSFWAA VVITSLLSVI PIWGPTIVTW IWSGFGVTGA TLKFFFVLHF
LLPWAILFIV LGHLIFLHST GSTSSLYCHG DYDKVCFSPE YIGKDAYNIV VWLVFIVLSL
IYPFNLGDAE MFIEADPMMS PVHIVPEWYF LFAYAILRAI PNKVLGVIAL LMSIVTFYFF
ALVNNYTSCL TKLNKFLVFL FIISSVILSW LGQCMVEDPF TVLSPLFSVI YFGLAYLLLG
IFMTSKLLFK
