CYB_DROSU
ID CYB_DROSU Reviewed; 166 AA.
AC P51941;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1996, sequence version 1.
DT 25-MAY-2022, entry version 93.
DE RecName: Full=Cytochrome b;
DE AltName: Full=Complex III subunit 3;
DE AltName: Full=Complex III subunit III;
DE AltName: Full=Cytochrome b-c1 complex subunit 3;
DE AltName: Full=Ubiquinol-cytochrome-c reductase complex cytochrome b subunit;
DE Flags: Fragment;
GN Name=mt:Cyt-b; Synonyms=Cob, cytb;
OS Drosophila subobscura (Fruit fly).
OG Mitochondrion.
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7241;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=7528808; DOI=10.1007/bf00173417;
RA Barrio E., Latorre A., Moya A.;
RT "Phylogeny of the Drosophila obscura species group deduced from
RT mitochondrial DNA sequences.";
RL J. Mol. Evol. 39:478-488(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 105-166.
RC STRAIN=Ssp. TUE 3;
RX PubMed=1454843; DOI=10.1073/pnas.89.23.11528;
RA Volz-Lingenhohl A., Solignac M., Sperlich D.;
RT "Stable heteroplasmy for a large-scale deletion in the coding region of
RT Drosophila subobscura mitochondrial DNA.";
RL Proc. Natl. Acad. Sci. U.S.A. 89:11528-11532(1992).
CC -!- FUNCTION: Component of the ubiquinol-cytochrome c reductase complex
CC (complex III or cytochrome b-c1 complex) that is part of the
CC mitochondrial respiratory chain. The b-c1 complex mediates electron
CC transfer from ubiquinol to cytochrome c. Contributes to the generation
CC of a proton gradient across the mitochondrial membrane that is then
CC used for ATP synthesis. {ECO:0000250|UniProtKB:P00157}.
CC -!- COFACTOR:
CC Name=heme; Xref=ChEBI:CHEBI:30413;
CC Evidence={ECO:0000250|UniProtKB:P00157};
CC Note=Binds 2 heme groups non-covalently.
CC {ECO:0000250|UniProtKB:P00157};
CC -!- SUBUNIT: The main subunits of complex b-c1 are: cytochrome b,
CC cytochrome c1 and the Rieske protein. {ECO:0000250|UniProtKB:P00157}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane
CC {ECO:0000250|UniProtKB:P00163}; Multi-pass membrane protein
CC {ECO:0000250|UniProtKB:P00163}.
CC -!- MISCELLANEOUS: Heme 1 (or BL or b562) is low-potential and absorbs at
CC about 562 nm, and heme 2 (or BH or b566) is high-potential and absorbs
CC at about 566 nm. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the cytochrome b family. {ECO:0000255|PROSITE-
CC ProRule:PRU00967}.
CC -!- CAUTION: The full-length protein contains only eight transmembrane
CC helices, not nine as predicted by bioinformatics tools.
CC {ECO:0000250|UniProtKB:P00157}.
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DR EMBL; U07287; AAA76624.1; -; Genomic_DNA.
DR EMBL; X65130; CAA46259.1; -; Genomic_DNA.
DR AlphaFoldDB; P51941; -.
DR SMR; P51941; -.
DR FlyBase; FBgn0012955; Dsub\mt:Cyt-b.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005743; C:mitochondrial inner membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0070469; C:respirasome; IEA:UniProtKB-KW.
DR GO; GO:0009055; F:electron transfer activity; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR CDD; cd00290; cytochrome_b_C; 1.
DR Gene3D; 1.20.810.10; -; 1.
DR InterPro; IPR005798; Cyt_b/b6_C.
DR InterPro; IPR036150; Cyt_b/b6_C_sf.
DR InterPro; IPR027387; Cytb/b6-like_sf.
DR Pfam; PF00032; Cytochrom_B_C; 1.
DR SUPFAM; SSF81648; SSF81648; 1.
DR PROSITE; PS51003; CYTB_CTER; 1.
PE 3: Inferred from homology;
KW Electron transport; Heme; Iron; Membrane; Metal-binding; Mitochondrion;
KW Mitochondrion inner membrane; Respiratory chain; Transmembrane;
KW Transmembrane helix; Transport; Ubiquinone.
FT CHAIN <1..166
FT /note="Cytochrome b"
FT /id="PRO_0000060897"
FT TRANSMEM 15..35
FT /note="Helical"
FT /evidence="ECO:0000250|UniProtKB:P00157"
FT TRANSMEM 77..97
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00967"
FT TRANSMEM 109..129
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00967"
FT TRANSMEM 136..156
FT /note="Helical"
FT /evidence="ECO:0000250|UniProtKB:P00157"
FT NON_TER 1
SQ SEQUENCE 166 AA; 19198 MW; E1CB117EF04B22A4 CRC64;
NSNIDKIPFH PYFTFKDIVG FIVMIFLLIS LVLINPNLLG DPDNFIPANP LVTPAHIQPE
WYFLFAYAIL RSIPNKLGGV IALVLSIAIL MILPFYNLSK FRGIQFYPIN QILFWIMLVT
VILLTWIGAR PVEEPYVLLG QILTVIYFLY YLINPLVSKW WDNLLN
