CYB_GLOSR
ID CYB_GLOSR Reviewed; 379 AA.
AC Q33487; Q8W7N1; Q8W816; Q8W8G0; Q8W8H9; Q8W901; Q8WGH2; Q8WGH3; Q8WGH4;
AC Q8WGH5; Q8WGH6; Q8WGH7; Q8WGH8; Q8WGH9; Q8WGI0; Q8WGI1; Q8WGI2; Q8WGI3;
AC Q8WGI4; Q8WGI5; Q8WGI6; Q8WGI7; Q8WGI8; Q8WGI9; Q8WGJ0; Q8WGJ1;
DT 15-JUL-1999, integrated into UniProtKB/Swiss-Prot.
DT 23-APR-2003, sequence version 2.
DT 03-AUG-2022, entry version 100.
DE RecName: Full=Cytochrome b;
DE AltName: Full=Complex III subunit 3;
DE AltName: Full=Complex III subunit III;
DE AltName: Full=Cytochrome b-c1 complex subunit 3;
DE AltName: Full=Ubiquinol-cytochrome-c reductase complex cytochrome b subunit;
GN Name=MT-CYB; Synonyms=COB, CYTB, MTCYB;
OS Glossophaga soricina (Pallas' long-tongued bat) (Vespertilio soricinus).
OG Mitochondrion.
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Chiroptera; Microchiroptera; Phyllostomidae;
OC Glossophaginae; Glossophaga.
OX NCBI_TaxID=27638;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA Hoffmann F.G., Baker R.J.;
RT "Systematics of bats of the genus Glossophaga (Chiroptera: Phyllostomidae)
RT and phylogeography in G. soricina based on the cytochrome b gene.";
RL J. Mammal. 82:1092-1101(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-176, AND VARIANTS MET-108 AND
RP MET-158.
RC STRAIN=Isolate LSUMZ 27407; TISSUE=Kidney, and Liver;
RA Sudman P.D., Barkley L.J., Hafner M.S.;
RT "Familial affinity of Tomopeas ravus (Chiroptera) based on protein
RT electrophoretic and cytochrome b sequence data.";
RL J. Mammal. 75:365-377(1994).
CC -!- FUNCTION: Component of the ubiquinol-cytochrome c reductase complex
CC (complex III or cytochrome b-c1 complex) that is part of the
CC mitochondrial respiratory chain. The b-c1 complex mediates electron
CC transfer from ubiquinol to cytochrome c. Contributes to the generation
CC of a proton gradient across the mitochondrial membrane that is then
CC used for ATP synthesis. {ECO:0000250|UniProtKB:P00157}.
CC -!- COFACTOR:
CC Name=heme b; Xref=ChEBI:CHEBI:60344;
CC Evidence={ECO:0000250|UniProtKB:P00157};
CC Note=Binds 2 heme b groups non-covalently.
CC {ECO:0000250|UniProtKB:P00157};
CC -!- SUBUNIT: The cytochrome bc1 complex contains 11 subunits: 3 respiratory
CC subunits (MT-CYB, CYC1 and UQCRFS1), 2 core proteins (UQCRC1 and
CC UQCRC2) and 6 low-molecular weight proteins (UQCRH/QCR6, UQCRB/QCR7,
CC UQCRQ/QCR8, UQCR10/QCR9, UQCR11/QCR10 and a cleavage product of
CC UQCRFS1). This cytochrome bc1 complex then forms a dimer.
CC {ECO:0000250|UniProtKB:P00157}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane
CC {ECO:0000250|UniProtKB:P00157}; Multi-pass membrane protein
CC {ECO:0000250|UniProtKB:P00157}.
CC -!- MISCELLANEOUS: Heme 1 (or BL or b562) is low-potential and absorbs at
CC about 562 nm, and heme 2 (or BH or b566) is high-potential and absorbs
CC at about 566 nm. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the cytochrome b family. {ECO:0000255|PROSITE-
CC ProRule:PRU00967, ECO:0000255|PROSITE-ProRule:PRU00968}.
CC -!- CAUTION: The full-length protein contains only eight transmembrane
CC helices, not nine as predicted by bioinformatics tools.
CC {ECO:0000250|UniProtKB:P00157}.
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DR EMBL; AF382830; AAL32304.1; -; Genomic_DNA.
DR EMBL; AF382831; AAL32305.1; -; Genomic_DNA.
DR EMBL; AF382832; AAL32306.1; -; Genomic_DNA.
DR EMBL; AF382833; AAL32307.1; -; Genomic_DNA.
DR EMBL; AF382834; AAL32308.1; -; Genomic_DNA.
DR EMBL; AF382835; AAL32309.1; -; Genomic_DNA.
DR EMBL; AF382836; AAL32310.1; -; Genomic_DNA.
DR EMBL; AF382837; AAL32311.1; -; Genomic_DNA.
DR EMBL; AF382838; AAL32312.1; -; Genomic_DNA.
DR EMBL; AF382839; AAL32313.1; -; Genomic_DNA.
DR EMBL; AF382840; AAL32314.1; -; Genomic_DNA.
DR EMBL; AF382841; AAL32315.1; -; Genomic_DNA.
DR EMBL; AF382842; AAL32316.1; -; Genomic_DNA.
DR EMBL; AF382843; AAL32317.1; -; Genomic_DNA.
DR EMBL; AF382844; AAL32318.1; -; Genomic_DNA.
DR EMBL; AF382845; AAL32319.1; -; Genomic_DNA.
DR EMBL; AF382846; AAL32320.1; -; Genomic_DNA.
DR EMBL; AF382847; AAL32321.1; -; Genomic_DNA.
DR EMBL; AF382848; AAL32322.1; -; Genomic_DNA.
DR EMBL; AF382849; AAL32323.1; -; Genomic_DNA.
DR EMBL; AF382850; AAL32324.1; -; Genomic_DNA.
DR EMBL; AF382851; AAL32325.1; -; Genomic_DNA.
DR EMBL; AF382852; AAL32326.1; -; Genomic_DNA.
DR EMBL; AF382853; AAL32327.1; -; Genomic_DNA.
DR EMBL; AF382854; AAL32328.1; -; Genomic_DNA.
DR EMBL; AF382855; AAL32329.1; -; Genomic_DNA.
DR EMBL; AF382856; AAL32330.1; -; Genomic_DNA.
DR EMBL; AF382857; AAL32331.1; -; Genomic_DNA.
DR EMBL; AF382858; AAL32332.1; -; Genomic_DNA.
DR EMBL; AF382859; AAL32333.1; -; Genomic_DNA.
DR EMBL; AF382860; AAL32334.1; -; Genomic_DNA.
DR EMBL; AF382861; AAL32335.1; -; Genomic_DNA.
DR EMBL; AF382862; AAL32336.1; -; Genomic_DNA.
DR EMBL; AF382863; AAL32337.1; -; Genomic_DNA.
DR EMBL; AF382864; AAL32338.1; -; Genomic_DNA.
DR EMBL; AF382865; AAL32339.1; -; Genomic_DNA.
DR EMBL; AF382866; AAL32340.1; -; Genomic_DNA.
DR EMBL; AF382867; AAL32341.1; -; Genomic_DNA.
DR EMBL; L19721; AAA17767.1; -; Genomic_DNA.
DR AlphaFoldDB; Q33487; -.
DR SMR; Q33487; -.
DR GO; GO:0005743; C:mitochondrial inner membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0045275; C:respiratory chain complex III; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0008121; F:ubiquinol-cytochrome-c reductase activity; IEA:InterPro.
DR GO; GO:0022904; P:respiratory electron transport chain; IEA:InterPro.
DR CDD; cd00290; cytochrome_b_C; 1.
DR CDD; cd00284; Cytochrome_b_N; 1.
DR Gene3D; 1.20.810.10; -; 1.
DR InterPro; IPR005798; Cyt_b/b6_C.
DR InterPro; IPR036150; Cyt_b/b6_C_sf.
DR InterPro; IPR005797; Cyt_b/b6_N.
DR InterPro; IPR027387; Cytb/b6-like_sf.
DR InterPro; IPR030689; Cytochrome_b.
DR InterPro; IPR016174; Di-haem_cyt_TM.
DR Pfam; PF00032; Cytochrom_B_C; 1.
DR Pfam; PF00033; Cytochrome_B; 1.
DR PIRSF; PIRSF038885; COB; 1.
DR SUPFAM; SSF81342; SSF81342; 1.
DR SUPFAM; SSF81648; SSF81648; 1.
DR PROSITE; PS51003; CYTB_CTER; 1.
DR PROSITE; PS51002; CYTB_NTER; 1.
PE 3: Inferred from homology;
KW Electron transport; Heme; Iron; Membrane; Metal-binding; Mitochondrion;
KW Mitochondrion inner membrane; Respiratory chain; Transmembrane;
KW Transmembrane helix; Transport; Ubiquinone.
FT CHAIN 1..379
FT /note="Cytochrome b"
FT /id="PRO_0000061008"
FT TRANSMEM 33..53
FT /note="Helical"
FT /evidence="ECO:0000250|UniProtKB:P00157"
FT TRANSMEM 77..98
FT /note="Helical"
FT /evidence="ECO:0000250|UniProtKB:P00157"
FT TRANSMEM 113..133
FT /note="Helical"
FT /evidence="ECO:0000250|UniProtKB:P00157"
FT TRANSMEM 178..198
FT /note="Helical"
FT /evidence="ECO:0000250|UniProtKB:P00157"
FT TRANSMEM 226..246
FT /note="Helical"
FT /evidence="ECO:0000250|UniProtKB:P00157"
FT TRANSMEM 288..308
FT /note="Helical"
FT /evidence="ECO:0000250|UniProtKB:P00157"
FT TRANSMEM 320..340
FT /note="Helical"
FT /evidence="ECO:0000250|UniProtKB:P00157"
FT TRANSMEM 347..367
FT /note="Helical"
FT /evidence="ECO:0000250|UniProtKB:P00157"
FT BINDING 83
FT /ligand="heme b"
FT /ligand_id="ChEBI:CHEBI:60344"
FT /ligand_label="b562"
FT /ligand_part="Fe"
FT /ligand_part_id="ChEBI:CHEBI:18248"
FT /note="axial binding residue"
FT /evidence="ECO:0000250|UniProtKB:P00157"
FT BINDING 97
FT /ligand="heme b"
FT /ligand_id="ChEBI:CHEBI:60344"
FT /ligand_label="b566"
FT /ligand_part="Fe"
FT /ligand_part_id="ChEBI:CHEBI:18248"
FT /note="axial binding residue"
FT /evidence="ECO:0000250|UniProtKB:P00157"
FT BINDING 182
FT /ligand="heme b"
FT /ligand_id="ChEBI:CHEBI:60344"
FT /ligand_label="b562"
FT /ligand_part="Fe"
FT /ligand_part_id="ChEBI:CHEBI:18248"
FT /note="axial binding residue"
FT /evidence="ECO:0000250|UniProtKB:P00157"
FT BINDING 196
FT /ligand="heme b"
FT /ligand_id="ChEBI:CHEBI:60344"
FT /ligand_label="b566"
FT /ligand_part="Fe"
FT /ligand_part_id="ChEBI:CHEBI:18248"
FT /note="axial binding residue"
FT /evidence="ECO:0000250|UniProtKB:P00157"
FT BINDING 201
FT /ligand="a ubiquinone"
FT /ligand_id="ChEBI:CHEBI:16389"
FT /evidence="ECO:0000250|UniProtKB:P00157"
FT VARIANT 14
FT /note="V -> I (in strain: Isolate FMNH 128675 and Isolate
FT FMNH 128718)"
FT VARIANT 23
FT /note="A -> T (in strain: Isolate FMNH 128713, Isolate TK
FT 4728, Isolate TK 86578 and Isolate TK 86579)"
FT VARIANT 26
FT /note="S -> N (in strain: Isolate TK 9251 and Isolate TK
FT 11040)"
FT VARIANT 39
FT /note="V -> A (in strain: Isolate MVZ 185575)"
FT VARIANT 39
FT /note="V -> M (in strain: Isolate FMNH 128718)"
FT VARIANT 78
FT /note="V -> M (in strain: Isolate TK 4728 and Isolate TK
FT 9251)"
FT VARIANT 108
FT /note="T -> M (in strain: Isolate FMNH 128676, Isolate FMNH
FT 128713, Isolate FMNH 128718 and Isolate TK 43208)"
FT /evidence="ECO:0000269|Ref.2"
FT VARIANT 110
FT /note="S -> L (in strain: Isolate TK 4728)"
FT VARIANT 119
FT /note="L -> M (in strain: Isolate TK 13173)"
FT VARIANT 121
FT /note="F -> L (in strain: Isolate MVZ 185583)"
FT VARIANT 132
FT /note="V -> D (in strain: Isolate TK 15194)"
FT VARIANT 132
FT /note="V -> I (in strain: Isolate MVZ 185566)"
FT VARIANT 156
FT /note="I -> N (in strain: Isolate TK 15194)"
FT VARIANT 158
FT /note="T -> M (in strain: Isolate FMNH 128676, Isolate FMNH
FT 128713, Isolate FMNH 128718, Isolate MVZ 185583, Isolate TK
FT 4728, Isolate TK 9251, Isolate TK 11040, Isolate TK 13173,
FT Isolate TK 13594, Isolate TK 14159, Isolate TK 34707,
FT Isolate TK 41573, Isolate TK 43138, Isolate TK 43178,
FT Isolate TK 43189, Isolate TK 43208, Isolate USNM578997,
FT Isolate USNM579009 and Isolate USNM579010)"
FT /evidence="ECO:0000269|Ref.2"
FT VARIANT 162
FT /note="Q -> H (in strain: Isolate TK 22631)"
FT VARIANT 176
FT /note="T -> K (in strain: Isolate TK 15194)"
FT VARIANT 185
FT /note="F -> L (in strain: Isolate TK 4728 and Isolate TK
FT 86578)"
FT VARIANT 215
FT /note="S -> P (in strain: Isolate TK 41573, Isolate TK
FT 86578, Isolate USNM578997, Isolate USNM579009 and Isolate
FT USNM579010)"
FT VARIANT 236
FT /note="L -> P (in strain: Isolate FMNH 128676)"
FT VARIANT 249
FT /note="L -> I (in strain: Isolate TK 41573)"
FT VARIANT 257
FT /note="M -> V (in strain: Isolate FMNH 128676)"
FT VARIANT 273
FT /note="Y -> F (in strain: Isolate TK 15194)"
FT VARIANT 303
FT /note="I -> V (in strain: Isolate TK 15194, Isolate TK
FT 22602, Isolate TK 22631, Isolate TK 25071, Isolate TK
FT 56869, Isolate USNM578997, Isolate USNM579009 and Isolate
FT USNM579010)"
FT VARIANT 331
FT /note="I -> F (in strain: Isolate FMNH 128676)"
FT VARIANT 338
FT /note="I -> V (in strain: Isolate FMNH 128718)"
FT VARIANT 356
FT /note="V -> I (in strain: Isolate TK 15154, Isolate TK
FT 15194, Isolate TK 17544, Isolate TK 25071 and Isolate TK
FT 25212)"
FT VARIANT 360
FT /note="L -> S (in strain: Isolate TK 86578)"
FT VARIANT 362..363
FT /note="IL -> TQ (in strain: Isolate FMNH 128676)"
FT VARIANT 364
FT /note="V -> I (in strain: Isolate TK 17544)"
FT VARIANT 369
FT /note="V -> I (in strain: Isolate TK 43189)"
FT VARIANT 375
FT /note="Y -> H (in strain: Isolate FMNH 128676, Isolate FMNH
FT 128713, Isolate FMNH 128718, Isolate TK 4728, Isolate TK
FT 9251, Isolate TK 11040, Isolate TK 13173, Isolate TK 13594,
FT Isolate TK 14159, Isolate TK 34707, Isolate TK 41573,
FT Isolate TK 43138, Isolate TK 43178, Isolate TK 43189,
FT Isolate TK 43208, Isolate USNM578997, Isolate USNM579009
FT and Isolate USNM579010)"
FT CONFLICT 135
FT /note="W -> C (in Ref. 2; AAA17767)"
FT /evidence="ECO:0000305"
FT CONFLICT 168..169
FT /note="FS -> SL (in Ref. 2; AAA17767)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 379 AA; 42770 MW; 206040C666641D34 CRC64;
MTNIRKTHPL LKIVNSSFVD LPAPSSLSSW WNFGSLLGVC LAVQILTGLF LAMHYTSDTA
TAFNSVTHIC RDVNYGWVLR YLHANGASMF FICLYLHVGR GLYYGSYTYS ETWNIGILLL
FAVMATAFMG YVLPWGQMSF WGATVITNLL SAIPYIGTDL VQWIWGGFSV DKATLTRFFA
FHFLFPFIVA ALVMVHLLFL HETGSNNPTG IPSDSDMIPF HPYYTIKDIL GFLIMLTALS
ALVLFSPDLL GDPDNYMPAN PLNTPPHIKP EWYFLFAYAI LRSIPNKLGG VLALVLSILI
LAIVPMLHTS KQRSMMFRPL SQCLFWFLVA ILFTLTWIGG QPVEYPYIII GQMASVLYFL
TILVFMPLVS IMENYLLKW
