ID   ACR2_NEUCR              Reviewed;         595 AA.
AC   P78704; Q7RVH5;
DT   27-APR-2001, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2004, sequence version 3.
DT   25-MAY-2022, entry version 133.
DE   RecName: Full=Acriflavine sensitivity control protein acr-2;
GN   Name=acr-2; ORFNames=NCU05733;
OS   Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 /
OS   FGSC 987).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC   Sordariomycetidae; Sordariales; Sordariaceae; Neurospora.
OX   NCBI_TaxID=367110;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=KH2;
RX   PubMed=8679704; DOI=10.1016/0167-4781(96)00042-5;
RA   Akiyama M., Nakashima H.;
RT   "Molecular cloning of the acr-2 gene which controls acriflavine sensitivity
RT   in Neurospora crassa.";
RL   Biochim. Biophys. Acta 1307:187-192(1996).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987;
RX   PubMed=12712197; DOI=10.1038/nature01554;
RA   Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D.,
RA   Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B.,
RA   Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M.,
RA   Qui D., Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M.,
RA   Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U.,
RA   Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D.,
RA   Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S.,
RA   Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D.,
RA   Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S.,
RA   Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A.,
RA   DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R.,
RA   Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R.,
RA   Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I.,
RA   Sachs M.S., Lander E.S., Nusbaum C., Birren B.W.;
RT   "The genome sequence of the filamentous fungus Neurospora crassa.";
RL   Nature 422:859-868(2003).
CC   -!- FUNCTION: Probable transcriptional regulator.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00227}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=EAA33653.2; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR   EMBL; D45893; BAA08308.1; -; Genomic_DNA.
DR   EMBL; CM002238; EAA33653.2; ALT_INIT; Genomic_DNA.
DR   PIR; S72537; S72537.
DR   RefSeq; XP_962889.2; XM_957796.2.
DR   AlphaFoldDB; P78704; -.
DR   STRING; 5141.EFNCRP00000007654; -.
DR   EnsemblFungi; EAA33653; EAA33653; NCU05733.
DR   GeneID; 3879039; -.
DR   KEGG; ncr:NCU05733; -.
DR   HOGENOM; CLU_020030_3_1_1; -.
DR   OMA; GQNPIRD; -.
DR   Proteomes; UP000001805; Chromosome 3, Linkage Group III.
DR   GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; IBA:GO_Central.
DR   GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IEA:InterPro.
DR   GO; GO:0000976; F:transcription cis-regulatory region binding; IBA:GO_Central.
DR   GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR   GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR   CDD; cd00067; GAL4; 1.
DR   Gene3D; 4.10.240.10; -; 1.
DR   InterPro; IPR021858; Fun_TF.
DR   InterPro; IPR001138; Zn2-C6_fun-type_DNA-bd.
DR   InterPro; IPR036864; Zn2-C6_fun-type_DNA-bd_sf.
DR   Pfam; PF11951; Fungal_trans_2; 1.
DR   Pfam; PF00172; Zn_clus; 1.
DR   SMART; SM00066; GAL4; 1.
DR   SUPFAM; SSF57701; SSF57701; 1.
DR   PROSITE; PS00463; ZN2_CY6_FUNGAL_1; 1.
DR   PROSITE; PS50048; ZN2_CY6_FUNGAL_2; 1.
PE   3: Inferred from homology;
KW   DNA-binding; Metal-binding; Nucleus; Reference proteome; Transcription;
KW   Transcription regulation; Zinc.
FT   CHAIN           1..595
FT                   /note="Acriflavine sensitivity control protein acr-2"
FT                   /id="PRO_0000114930"
FT   DNA_BIND        22..49
FT                   /note="Zn(2)-C6 fungal-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00227"
FT   REGION          69..172
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        69..152
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   VARIANT         303
FT                   /note="N -> K (confers acriflavine-resistance)"
FT   CONFLICT        204
FT                   /note="F -> V (in Ref. 1; BAA08308)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   595 AA;  65326 MW;  16CEE2FE22E93F00 CRC64;
     MALKKMPPKK PYHQKASKTK ACYNCHRKRL RCDKSLPACL KCSINGEECL GYGIVLRWAA
     CNSPTSTITT RTTNKTNFNG TNTTTPRTVK SSTPTQAPTP SDSPRQLDTD VTSSSAPSHT
     CSRSTTTSTT TTRISSPTLE ETIDSFTVET PIDNNVDPLP RAPDDNPDPS SQIIKRPVNL
     IKIPLTDPLL NGLSTKARWY MHHFATIVCR DLVSIDQKER NPFRAIIPLV RKFDYLQSVV
     LATAAMHLST IHKYQGRSLP SESALVDALM LKSRALHLLR AAINDNTLTD KAMILSAIVF
     LVNLDLIDSG RGGWKAHVGA ARRLISSLYL TKAHLDGAIA PLVNAIAADC LTYRIYGSTI
     SGNTSSWSDN TIDDGVVLPY ILQNAEAYSY HCAPPAILQI ILSASQLCSG SSTTLETDGG
     AGRIVTAAAL LHKARNFDVQ TWVYNIKGLP PDDDLEARVS VASAHRAAAC LFVLLSVPET
     GLLEIPLLEP KDLVQEILGH LSCIPDNHVH LKGTVWPTFV VGAETDDLSE RAWCLERLVA
     VWTKNPWTYP WGYVHTAMEM LQEIWRLKDL AAQQGDDGIN WLQRLKATEN SCLIV