CYB_MAMPR
ID CYB_MAMPR Reviewed; 378 AA.
AC P92658; Q35052; Q35053; Q36723; Q38PR0;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 27-JAN-2003, sequence version 3.
DT 03-AUG-2022, entry version 105.
DE RecName: Full=Cytochrome b;
DE AltName: Full=Complex III subunit 3;
DE AltName: Full=Complex III subunit III;
DE AltName: Full=Cytochrome b-c1 complex subunit 3;
DE AltName: Full=Ubiquinol-cytochrome-c reductase complex cytochrome b subunit;
GN Name=MT-CYB; Synonyms=COB, CYTB, MTCYB;
OS Mammuthus primigenius (Siberian woolly mammoth).
OG Mitochondrion.
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Afrotheria; Proboscidea; Elephantidae; Mammuthus.
OX NCBI_TaxID=37349;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC TISSUE=Muscle;
RX PubMed=9493356; DOI=10.1007/pl00006308;
RA Noro M., Masuda R., Dubrovo I.A., Yoshida M.C., Kato M.;
RT "Molecular phylogenetic inference of the woolly mammoth Mammuthus
RT primigenius, based on complete sequences of mitochondrial cytochrome b and
RT 12S ribosomal RNA genes.";
RL J. Mol. Evol. 46:314-326(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=16362058; DOI=10.1038/nature04432;
RA Krause J., Dear P.H., Pollack J.L., Slatkin M., Spriggs H., Barnes I.,
RA Lister A.M., Ebersberger I., Paeaebo S., Hofreiter M.;
RT "Multiplex amplification of the mammoth mitochondrial genome and the
RT evolution of Elephantidae.";
RL Nature 439:724-727(2006).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC TISSUE=Muscle;
RX PubMed=16448217; DOI=10.1371/journal.pbio.0040073;
RA Rogaev E.I., Moliaka Y.K., Malyarchuk B.A., Kondrashov F.A., Derenko M.V.,
RA Chumakov I., Grigorenko A.P.;
RT "Complete mitochondrial genome and phylogeny of Pleistocene mammoth
RT Mammuthus primigenius.";
RL PLoS Biol. 4:403-410(2006).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-335.
RC TISSUE=Muscle;
RX PubMed=9089080; DOI=10.1007/pl00006160;
RA Ozawa T., Hayashi S., Mikhelson V.M.;
RT "Phylogenetic position of mammoth and Steller's sea cow within Tethytheria
RT demonstrated by mitochondrial DNA sequences.";
RL J. Mol. Evol. 44:406-413(1997).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 34-143.
RA Derenko M., Malyarchuk B., Shields G.F.;
RL Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 32-106.
RC STRAIN=Isolate allele Fairbank, and Isolate allele Lyakhovskiy;
RA Yang H., Golenberg E.M., Shoshani J.;
RL Submitted (AUG-1995) to the EMBL/GenBank/DDBJ databases.
RN [7]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 33-114.
RC TISSUE=Bone;
RX PubMed=8047136; DOI=10.1038/370333b0;
RA Hagelberg E., Thomas M.G., Cook C.E. Jr., Sher A.V., Baryshnikov G.F.,
RA Lister A.M.;
RT "DNA from ancient mammoth bones.";
RL Nature 370:333-334(1994).
CC -!- FUNCTION: Component of the ubiquinol-cytochrome c reductase complex
CC (complex III or cytochrome b-c1 complex) that is part of the
CC mitochondrial respiratory chain. The b-c1 complex mediates electron
CC transfer from ubiquinol to cytochrome c. Contributes to the generation
CC of a proton gradient across the mitochondrial membrane that is then
CC used for ATP synthesis. {ECO:0000250|UniProtKB:P00157}.
CC -!- COFACTOR:
CC Name=heme b; Xref=ChEBI:CHEBI:60344;
CC Evidence={ECO:0000250|UniProtKB:P00157};
CC Note=Binds 2 heme b groups non-covalently.
CC {ECO:0000250|UniProtKB:P00157};
CC -!- SUBUNIT: The cytochrome bc1 complex contains 11 subunits: 3 respiratory
CC subunits (MT-CYB, CYC1 and UQCRFS1), 2 core proteins (UQCRC1 and
CC UQCRC2) and 6 low-molecular weight proteins (UQCRH/QCR6, UQCRB/QCR7,
CC UQCRQ/QCR8, UQCR10/QCR9, UQCR11/QCR10 and a cleavage product of
CC UQCRFS1). This cytochrome bc1 complex then forms a dimer.
CC {ECO:0000250|UniProtKB:P00157}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane
CC {ECO:0000250|UniProtKB:P00157}; Multi-pass membrane protein
CC {ECO:0000250|UniProtKB:P00157}.
CC -!- MISCELLANEOUS: Heme 1 (or BL or b562) is low-potential and absorbs at
CC about 562 nm, and heme 2 (or BH or b566) is high-potential and absorbs
CC at about 566 nm. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the cytochrome b family. {ECO:0000255|PROSITE-
CC ProRule:PRU00967, ECO:0000255|PROSITE-ProRule:PRU00968}.
CC -!- CAUTION: The full-length protein contains only eight transmembrane
CC helices, not nine as predicted by bioinformatics tools.
CC {ECO:0000250|UniProtKB:P00157}.
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DR EMBL; D50842; BAA25008.1; -; Genomic_DNA.
DR EMBL; DQ188829; ABA29796.1; -; Genomic_DNA.
DR EMBL; DQ316067; ABC17890.1; -; Genomic_DNA.
DR EMBL; D83047; BAA20277.1; -; Genomic_DNA.
DR EMBL; U79411; AAB38284.1; -; Genomic_DNA.
DR EMBL; U23738; AAA73786.1; -; Genomic_DNA.
DR EMBL; U23739; AAA73787.1; -; Genomic_DNA.
DR EMBL; S72502; AAD14109.1; -; Genomic_DNA.
DR RefSeq; YP_398766.1; NC_007596.2.
DR AlphaFoldDB; P92658; -.
DR SMR; P92658; -.
DR GeneID; 3773153; -.
DR CTD; 4519; -.
DR GO; GO:0005743; C:mitochondrial inner membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0045275; C:respiratory chain complex III; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0008121; F:ubiquinol-cytochrome-c reductase activity; IEA:InterPro.
DR GO; GO:0022904; P:respiratory electron transport chain; IEA:InterPro.
DR CDD; cd00290; cytochrome_b_C; 1.
DR CDD; cd00284; Cytochrome_b_N; 1.
DR Gene3D; 1.20.810.10; -; 1.
DR InterPro; IPR005798; Cyt_b/b6_C.
DR InterPro; IPR036150; Cyt_b/b6_C_sf.
DR InterPro; IPR005797; Cyt_b/b6_N.
DR InterPro; IPR027387; Cytb/b6-like_sf.
DR InterPro; IPR030689; Cytochrome_b.
DR InterPro; IPR016174; Di-haem_cyt_TM.
DR Pfam; PF00032; Cytochrom_B_C; 1.
DR Pfam; PF00033; Cytochrome_B; 1.
DR PIRSF; PIRSF038885; COB; 1.
DR SUPFAM; SSF81342; SSF81342; 1.
DR SUPFAM; SSF81648; SSF81648; 1.
DR PROSITE; PS51003; CYTB_CTER; 1.
DR PROSITE; PS51002; CYTB_NTER; 1.
PE 3: Inferred from homology;
KW Electron transport; Extinct organism protein; Heme; Iron; Membrane;
KW Metal-binding; Mitochondrion; Mitochondrion inner membrane;
KW Respiratory chain; Transmembrane; Transmembrane helix; Transport;
KW Ubiquinone.
FT CHAIN 1..378
FT /note="Cytochrome b"
FT /id="PRO_0000061150"
FT TRANSMEM 33..53
FT /note="Helical"
FT /evidence="ECO:0000250|UniProtKB:P00157"
FT TRANSMEM 77..98
FT /note="Helical"
FT /evidence="ECO:0000250|UniProtKB:P00157"
FT TRANSMEM 113..133
FT /note="Helical"
FT /evidence="ECO:0000250|UniProtKB:P00157"
FT TRANSMEM 178..198
FT /note="Helical"
FT /evidence="ECO:0000250|UniProtKB:P00157"
FT TRANSMEM 226..246
FT /note="Helical"
FT /evidence="ECO:0000250|UniProtKB:P00157"
FT TRANSMEM 288..308
FT /note="Helical"
FT /evidence="ECO:0000250|UniProtKB:P00157"
FT TRANSMEM 320..340
FT /note="Helical"
FT /evidence="ECO:0000250|UniProtKB:P00157"
FT TRANSMEM 347..367
FT /note="Helical"
FT /evidence="ECO:0000250|UniProtKB:P00157"
FT BINDING 83
FT /ligand="heme b"
FT /ligand_id="ChEBI:CHEBI:60344"
FT /ligand_label="b562"
FT /ligand_part="Fe"
FT /ligand_part_id="ChEBI:CHEBI:18248"
FT /note="axial binding residue"
FT /evidence="ECO:0000250|UniProtKB:P00157"
FT BINDING 97
FT /ligand="heme b"
FT /ligand_id="ChEBI:CHEBI:60344"
FT /ligand_label="b566"
FT /ligand_part="Fe"
FT /ligand_part_id="ChEBI:CHEBI:18248"
FT /note="axial binding residue"
FT /evidence="ECO:0000250|UniProtKB:P00157"
FT BINDING 182
FT /ligand="heme b"
FT /ligand_id="ChEBI:CHEBI:60344"
FT /ligand_label="b562"
FT /ligand_part="Fe"
FT /ligand_part_id="ChEBI:CHEBI:18248"
FT /note="axial binding residue"
FT /evidence="ECO:0000250|UniProtKB:P00157"
FT BINDING 196
FT /ligand="heme b"
FT /ligand_id="ChEBI:CHEBI:60344"
FT /ligand_label="b566"
FT /ligand_part="Fe"
FT /ligand_part_id="ChEBI:CHEBI:18248"
FT /note="axial binding residue"
FT /evidence="ECO:0000250|UniProtKB:P00157"
FT BINDING 201
FT /ligand="a ubiquinone"
FT /ligand_id="ChEBI:CHEBI:16389"
FT /evidence="ECO:0000250|UniProtKB:P00157"
FT CONFLICT 95..96
FT /note="Missing (in Ref. 7; AAD14109)"
FT /evidence="ECO:0000305"
FT CONFLICT 218
FT /note="I -> V (in Ref. 4; BAA20277)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 378 AA; 42777 MW; 786EDA681CDB0958 CRC64;
MTHIRKSHPL LKILNKSFID LPTPSNISTW WNFGSLLGAC LITQILTGLF LAMHYTPDTM
TAFSSMSHIC RDVNYGWIIR QLHSNGASIF FLCLYTHIGR NIYYGSYLYS ETWNTGIMLL
LITMATAFMG YVLPWGQMSF WGATVITNLF SAIPYIGTDL VEWIWGGFSV DKATLNRFFA
LHFILPFTMI ALAGVHLTFL HETGSNNPLG LTSDSDKIPF HPYYTIKDFL GLLILILFLL
LLALLSPDML GDPDNYMPAD PLNTPLHIKP EWYFLFAYAI LRSVPNKLGG VLALLLSILI
LGIMPLLHTS KHRSMMLRPL SQVLFWTLAT DLLMLTWIGS QPVEYPYIII GQMASILYFS
IILAFLPIAG MIENYLIK
