ID   ACRA_ECO57              Reviewed;         397 AA.
AC   P0AE07; P31223;
DT   06-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT   06-DEC-2005, sequence version 1.
DT   03-AUG-2022, entry version 100.
DE   RecName: Full=Multidrug efflux pump subunit AcrA;
DE   AltName: Full=AcrAB-TolC multidrug efflux pump subunit AcrA;
DE   AltName: Full=Acriflavine resistance protein A;
DE   Flags: Precursor;
GN   Name=acrA; OrderedLocusNames=Z0578, ECs0516;
OS   Escherichia coli O157:H7.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83334;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=O157:H7 / EDL933 / ATCC 700927 / EHEC;
RX   PubMed=11206551; DOI=10.1038/35054089;
RA   Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J.,
RA   Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G.,
RA   Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J.,
RA   Davis N.W., Lim A., Dimalanta E.T., Potamousis K., Apodaca J.,
RA   Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A.,
RA   Blattner F.R.;
RT   "Genome sequence of enterohaemorrhagic Escherichia coli O157:H7.";
RL   Nature 409:529-533(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=O157:H7 / Sakai / RIMD 0509952 / EHEC;
RX   PubMed=11258796; DOI=10.1093/dnares/8.1.11;
RA   Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K.,
RA   Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T.,
RA   Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T., Kuhara S.,
RA   Shiba T., Hattori M., Shinagawa H.;
RT   "Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and
RT   genomic comparison with a laboratory strain K-12.";
RL   DNA Res. 8:11-22(2001).
CC   -!- FUNCTION: AcrA-AcrB-AcrZ-TolC is a drug efflux protein complex with
CC       broad substrate specificity that uses the proton motive force to export
CC       substrates. This subunit may act as an adapter protein that links AcrB
CC       and TolC stably together (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Monomeric in solution. Homotrimeric; interacts independently
CC       with AcrB and TolC as well as AcrZ. Part of the AcrA-AcrB-TolC efflux
CC       pump (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000250}; Lipid-anchor
CC       {ECO:0000255|PROSITE-ProRule:PRU00303}.
CC   -!- SIMILARITY: Belongs to the membrane fusion protein (MFP) (TC 8.A.1)
CC       family. {ECO:0000305}.
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DR   EMBL; AE005174; AAG54812.1; -; Genomic_DNA.
DR   EMBL; BA000007; BAB33939.1; -; Genomic_DNA.
DR   PIR; D90693; D90693.
DR   PIR; H85543; H85543.
DR   RefSeq; NP_308543.1; NC_002695.1.
DR   RefSeq; WP_001295324.1; NZ_SWKA01000005.1.
DR   AlphaFoldDB; P0AE07; -.
DR   SMR; P0AE07; -.
DR   STRING; 155864.EDL933_0538; -.
DR   EnsemblBacteria; AAG54812; AAG54812; Z0578.
DR   EnsemblBacteria; BAB33939; BAB33939; ECs_0516.
DR   GeneID; 66671235; -.
DR   GeneID; 914620; -.
DR   KEGG; ece:Z0578; -.
DR   KEGG; ecs:ECs_0516; -.
DR   PATRIC; fig|386585.9.peg.621; -.
DR   eggNOG; COG0845; Bacteria.
DR   HOGENOM; CLU_018816_2_1_6; -.
DR   Proteomes; UP000000558; Chromosome.
DR   Proteomes; UP000002519; Chromosome.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0022857; F:transmembrane transporter activity; IEA:InterPro.
DR   GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR   InterPro; IPR043602; CusB_dom_1.
DR   InterPro; IPR032317; HlyD_D23.
DR   InterPro; IPR006143; RND_pump_MFP.
DR   Pfam; PF00529; CusB_dom_1; 1.
DR   Pfam; PF16576; HlyD_D23; 1.
DR   TIGRFAMs; TIGR01730; RND_mfp; 1.
DR   PROSITE; PS51257; PROKAR_LIPOPROTEIN; 1.
PE   3: Inferred from homology;
KW   Antibiotic resistance; Cell inner membrane; Cell membrane; Coiled coil;
KW   Lipoprotein; Membrane; Palmitate; Reference proteome; Signal; Transport.
FT   SIGNAL          1..24
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT   CHAIN           25..397
FT                   /note="Multidrug efflux pump subunit AcrA"
FT                   /id="PRO_0000043378"
FT   REGION          377..397
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COILED          98..172
FT                   /evidence="ECO:0000250"
FT   LIPID           25
FT                   /note="N-palmitoyl cysteine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT   LIPID           25
FT                   /note="S-diacylglycerol cysteine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
SQ   SEQUENCE   397 AA;  42197 MW;  5B81DD5BC2B0A077 CRC64;
     MNKNRGFTPL AVVLMLSGSL ALTGCDDKQA QQGGQQMPAV GVVTVKTEPL QITTELPGRT
     SAYRIAEVRP QVSGIILKRN FKEGSDIEAG VSLYQIDPAT YQATYDSAKG DLAKAQAAAN
     IAQLTVNRYQ KLLGTQYISK QEYDQALADA QQANAAVTAA KAAVETARIN LAYTKVTSPI
     SGRIGKSNVT EGALVQNGQA TALATVQQLD PIYVDVTQSS NDFLRLKQEL ANGTLKQENG
     KAKVSLITSD GIKFPQDGTL EFSDVTVDQT TGSITLRAIF PNPDHTLLPG MFVRARLEEG
     LNPNAILVPQ QGVTRTPRGD ATVLVVGADD KVETRPIVAS QAIGDKWLVT EGLKAGDRVV
     ISGLQKVRPG VQVKAQEVTA DNNQQAASGA QPEQSKS