ACRBP_HUMAN
ID ACRBP_HUMAN Reviewed; 543 AA.
AC Q8NEB7; Q9BY87;
DT 07-MAR-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 1.
DT 03-AUG-2022, entry version 136.
DE RecName: Full=Acrosin-binding protein;
DE AltName: Full=Acrosin-binding protein, 60 kDa form;
DE AltName: Full=Cancer/testis antigen 23;
DE Short=CT23;
DE AltName: Full=Cancer/testis antigen OY-TES-1 {ECO:0000303|PubMed:11248070};
DE AltName: Full=Proacrosin-binding protein sp32;
DE Contains:
DE RecName: Full=Acrosin-binding protein, mature form;
DE AltName: Full=Acrosin-binding protein, 32 kDa form, mature form;
DE Flags: Precursor;
GN Name=ACRBP {ECO:0000312|EMBL:AAH33010.1};
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1] {ECO:0000305, ECO:0000312|EMBL:BAB39388.1}
RP NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
RC TISSUE=Testis {ECO:0000312|EMBL:BAB39388.1};
RX PubMed=11248070; DOI=10.1073/pnas.041625098;
RA Ono T., Kurashige T., Harada N., Noguchi Y., Saika T., Niikawa N., Aoe M.,
RA Nakamura S., Higashi T., Hiraki A., Wada H., Kumon H., Old L.J.,
RA Nakayama E.;
RT "Identification of proacrosin binding protein sp32 precursor as a human
RT cancer/testis antigen.";
RL Proc. Natl. Acad. Sci. U.S.A. 98:3282-3287(2001).
RN [2] {ECO:0000312|EMBL:AAH33010.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Testis {ECO:0000312|EMBL:AAH33010.1};
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: [Acrosin-binding protein, mature form]: Acrosomal protein
CC that maintains proacrosin (pro-ACR) as an enzymatically inactive
CC zymogen in the acrosome. Involved also in the acrosome formation.
CC {ECO:0000250|UniProtKB:Q3V140}.
CC -!- SUBUNIT: [Acrosin-binding protein, mature form]: Binds proacrosin (pro-
CC ACR). Does not bind the mature form of ACR.
CC {ECO:0000250|UniProtKB:Q29016}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250|UniProtKB:Q29016}.
CC Cytoplasmic vesicle, secretory vesicle, acrosome
CC {ECO:0000250|UniProtKB:Q29016, ECO:0000250|UniProtKB:Q3V140}.
CC -!- TISSUE SPECIFICITY: Expression restricted to testis in normal tissue.
CC Expressed in a wide spectrum of cancers, including bladder, breast,
CC liver, lung and colon cancers. {ECO:0000269|PubMed:11248070}.
CC -!- PTM: Phosphorylated on Tyr residues in capacitated sperm.
CC {ECO:0000250|UniProtKB:Q29016}.
CC -!- PTM: The N-terminus is blocked. {ECO:0000250|UniProtKB:Q29016}.
CC -!- PTM: Synthesized as a 60-kDa precursor, the 32-kDa mature form is post-
CC translationally produced by the removal of the N-terminal half of the
CC precursor during sperm maturation in the testis and/or epididymis.
CC {ECO:0000250|UniProtKB:Q29016}.
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DR EMBL; AB051833; BAB39388.1; -; mRNA.
DR EMBL; BC033010; AAH33010.1; -; mRNA.
DR CCDS; CCDS8554.1; -.
DR RefSeq; NP_115878.2; NM_032489.2.
DR AlphaFoldDB; Q8NEB7; -.
DR BioGRID; 124113; 23.
DR STRING; 9606.ENSP00000229243; -.
DR iPTMnet; Q8NEB7; -.
DR PhosphoSitePlus; Q8NEB7; -.
DR BioMuta; ACRBP; -.
DR DMDM; 74730232; -.
DR MassIVE; Q8NEB7; -.
DR PaxDb; Q8NEB7; -.
DR PeptideAtlas; Q8NEB7; -.
DR PRIDE; Q8NEB7; -.
DR ProteomicsDB; 73146; -.
DR Antibodypedia; 48711; 91 antibodies from 21 providers.
DR DNASU; 84519; -.
DR Ensembl; ENST00000229243.7; ENSP00000229243.2; ENSG00000111644.8.
DR GeneID; 84519; -.
DR KEGG; hsa:84519; -.
DR MANE-Select; ENST00000229243.7; ENSP00000229243.2; NM_032489.3; NP_115878.2.
DR UCSC; uc001qpu.2; human.
DR CTD; 84519; -.
DR DisGeNET; 84519; -.
DR GeneCards; ACRBP; -.
DR HGNC; HGNC:17195; ACRBP.
DR HPA; ENSG00000111644; Tissue enriched (testis).
DR MIM; 608352; gene.
DR neXtProt; NX_Q8NEB7; -.
DR OpenTargets; ENSG00000111644; -.
DR PharmGKB; PA24452; -.
DR VEuPathDB; HostDB:ENSG00000111644; -.
DR eggNOG; ENOG502R6TS; Eukaryota.
DR GeneTree; ENSGT00390000000826; -.
DR HOGENOM; CLU_534141_0_0_1; -.
DR InParanoid; Q8NEB7; -.
DR OMA; CPLCAFC; -.
DR OrthoDB; 961418at2759; -.
DR PhylomeDB; Q8NEB7; -.
DR TreeFam; TF336597; -.
DR PathwayCommons; Q8NEB7; -.
DR BioGRID-ORCS; 84519; 20 hits in 1078 CRISPR screens.
DR ChiTaRS; ACRBP; human.
DR GenomeRNAi; 84519; -.
DR Pharos; Q8NEB7; Tbio.
DR PRO; PR:Q8NEB7; -.
DR Proteomes; UP000005640; Chromosome 12.
DR RNAct; Q8NEB7; protein.
DR Bgee; ENSG00000111644; Expressed in left testis and 116 other tissues.
DR ExpressionAtlas; Q8NEB7; baseline and differential.
DR Genevisible; Q8NEB7; HS.
DR GO; GO:0002080; C:acrosomal membrane; ISS:UniProtKB.
DR GO; GO:0001669; C:acrosomal vesicle; ISS:UniProtKB.
DR GO; GO:0005576; C:extracellular region; ISS:UniProtKB.
DR GO; GO:0005634; C:nucleus; HDA:UniProtKB.
DR GO; GO:0001675; P:acrosome assembly; ISS:UniProtKB.
DR GO; GO:0009566; P:fertilization; ISS:UniProtKB.
DR GO; GO:0007286; P:spermatid development; ISS:UniProtKB.
DR InterPro; IPR036058; Kazal_dom_sf.
DR InterPro; IPR009865; Proacrosin-bd.
DR PANTHER; PTHR21362; PTHR21362; 1.
DR Pfam; PF07222; PBP_sp32; 1.
DR SUPFAM; SSF100895; SSF100895; 1.
PE 2: Evidence at transcript level;
KW Cytoplasmic vesicle; Phosphoprotein; Reference proteome; Secreted; Signal.
FT SIGNAL 1..25
FT /evidence="ECO:0000255"
FT CHAIN 26..543
FT /note="Acrosin-binding protein"
FT /id="PRO_0000227516"
FT PROPEP 26..273
FT /note="Removed in mature form"
FT /evidence="ECO:0000250|UniProtKB:Q29016"
FT /id="PRO_0000449367"
FT CHAIN 274..543
FT /note="Acrosin-binding protein, mature form"
FT /id="PRO_0000449368"
FT REGION 26..106
FT /note="Pro-ACR binding"
FT /evidence="ECO:0000250|UniProtKB:Q3V140"
FT REGION 185..272
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 319..427
FT /note="Pro-ACR binding"
FT /evidence="ECO:0000250|UniProtKB:Q3V140"
FT COMPBIAS 195..219
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 242..271
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VARIANT 336
FT /note="T -> A (in dbSNP:rs3741923)"
FT /id="VAR_050633"
FT CONFLICT 32
FT /note="S -> P (in Ref. 1; BAB39388)"
FT /evidence="ECO:0000305"
FT CONFLICT 316
FT /note="L -> F (in Ref. 1; BAB39388)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 543 AA; 61359 MW; 44D0F23583522DD9 CRC64;
MRKPAAGFLP SLLKVLLLPL APAAAQDSTQ ASTPGSPLSP TEYERFFALL TPTWKAETTC
RLRATHGCRN PTLVQLDQYE NHGLVPDGAV CSNLPYASWF ESFCQFTHYR CSNHVYYAKR
VLCSQPVSIL SPNTLKEIEA SAEVSPTTMT SPISPHFTVT ERQTFQPWPE RLSNNVEELL
QSSLSLGGQE QAPEHKQEQG VEHRQEPTQE HKQEEGQKQE EQEEEQEEEG KQEEGQGTKE
GREAVSQLQT DSEPKFHSES LSSNPSSFAP RVREVESTPM IMENIQELIR SAQEIDEMNE
IYDENSYWRN QNPGSLLQLP HTEALLVLCY SIVENTCIIT PTAKAWKYME EEILGFGKSV
CDSLGRRHMS TCALCDFCSL KLEQCHSEAS LQRQQCDTSH KTPFVSPLLA SQSLSIGNQV
GSPESGRFYG LDLYGGLHMD FWCARLATKG CEDVRVSGWL QTEFLSFQDG DFPTKICDTD
YIQYPNYCSF KSQQCLMRNR NRKVSRMRCL QNETYSALSP GKSEDVVLRW SQEFSTLTLG
QFG
