CYB_PIG
ID CYB_PIG Reviewed; 379 AA.
AC P24964; O78725; Q35918; Q36542; Q36550; Q36979; Q37077; Q94PU4; Q9T433;
DT 01-MAR-1992, integrated into UniProtKB/Swiss-Prot.
DT 15-DEC-1998, sequence version 2.
DT 03-AUG-2022, entry version 147.
DE RecName: Full=Cytochrome b;
DE AltName: Full=Complex III subunit 3;
DE AltName: Full=Complex III subunit III;
DE AltName: Full=Cytochrome b-c1 complex subunit 3;
DE AltName: Full=Ubiquinol-cytochrome-c reductase complex cytochrome b subunit;
GN Name=MT-CYB; Synonyms=COB, CYTB, MTCYB;
OS Sus scrofa (Pig).
OG Mitochondrion.
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Suina; Suidae; Sus.
OX NCBI_TaxID=9823;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=1901092; DOI=10.1007/bf02515385;
RA Irwin D.M., Kocher T.D., Wilson A.C.;
RT "Evolution of the cytochrome b gene of mammals.";
RL J. Mol. Evol. 32:128-144(1991).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=9732457; DOI=10.1007/pl00006388;
RA Ursing B.M., Arnason U.;
RT "The complete mitochondrial DNA sequence of the pig (Sus scrofa).";
RL J. Mol. Evol. 47:302-306(1998).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Landrace;
RX PubMed=10433971; DOI=10.1016/s0378-1119(99)00247-4;
RA Lin C.S., Sun Y.L., Liu C.Y., Yang P.C., Chang L.C., Cheng I.C.,
RA Mao S.J.T., Huang M.C.;
RT "Complete nucleotide sequence of pig (Sus scrofa) mitochondrial genome and
RT dating evolutionary divergence within artiodactyla.";
RL Gene 236:107-114(1999).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=Various strains; TISSUE=Liver;
RA Randi E., Lucchini V., Diong C.;
RT "Evolutionary genetics of the suiformes.";
RL J. Mammal. Evol. 3:163-194(1996).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=Various strains;
RA Watanobe T., Okumura N., Ishiguro N., Nakano M., Matsui A., Sahara M.,
RA Komatsu M.;
RT "Genetic relationship and distribution of Japanese wild boar (Sus scrofa
RT leucomystax) and Ryukyu wild boar (Sus scrofa riukiuanus) analyzed by
RT mitochondrial DNA.";
RL Submitted (MAY-1998) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=AWB10, AWB11, Cook islands, EWB1, EWB2, EWB3, H1, H2,
RC Israel Wild Boar 1, L1, L2, LW1, LW2, and Ma;
RX PubMed=10747069; DOI=10.1093/genetics/154.4.1785;
RA Giuffra E., Kijas J.M.H., Amarger V., Carlborg O., Jeon J.-T.,
RA Andersson L.;
RT "The origin of the domestic pig: independent domestication and subsequent
RT introgression.";
RL Genetics 154:1785-1791(2000).
RN [7]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=Swedish wild boar;
RX PubMed=11428467; DOI=10.1007/s002390010158;
RA Kijas J.M.H., Andersson L.;
RT "A phylogenetic study of the origin of the domestic pig estimated from the
RT near-complete mtDNA genome.";
RL J. Mol. Evol. 52:302-308(2001).
RN [8]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=Ssc1, Ssc2, and Ssc3;
RA Randi E., d'Huart J.P., Lucchini V., Aman R.;
RT "Evidence of two genetically deeply divergent species of warthog,
RT Phacochoerus africanus and P. aethiopicus (Artiodactyla: Suiformes) in East
RT Africa.";
RL Submitted (MAY-2001) to the EMBL/GenBank/DDBJ databases.
RN [9]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=Basque, D1, D2, D3, D4, D6, D7, Hungarian Mangalitza, IB1, IB10,
RC IB11, IB13, IB14, IB15, IB16, IB17, IB18, IB19, IB2, IB20, IB21, IB22,
RC IB23, IB24, IB25, IB26, IB4, IB5, IB6, IB7, IB8, IB9, LR1, LR2, P1, P2,
RC SWB1, SWB2, SWB3, SWB4, SWB5, and SWB6;
RX PubMed=14510666; DOI=10.1046/j.1365-2052.2003.01010.x;
RA Alves E., Ovilo C., Rodriguez M.C., Silio L.;
RT "Mitochondrial DNA sequence variation and phylogenetic relationships among
RT Iberian pigs and other domestic and wild pig populations.";
RL Anim. Genet. 34:319-324(2003).
RN [10]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=Chinese Diannan short-ear, Duroc, and Landrace;
RX PubMed=14557390; DOI=10.1093/jhered/esg077;
RA Yang J., Wang J., Kijas J., Liu B., Han H., Yu M., Yang H., Zhao S., Li K.;
RT "Genetic diversity present within the near-complete mtDNA genome of 17
RT breeds of indigenous Chinese pigs.";
RL J. Hered. 94:381-385(2003).
RN [11]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 288-379.
RC STRAIN=Meishan; TISSUE=Liver;
RX PubMed=7486249; DOI=10.1111/j.1365-2052.1995.tb02666.x;
RA Takeda K., Onishi A., Ishida N., Kawakami K., Komatsu M., Inumaru S.;
RT "SSCP analysis of pig mitochondrial DNA D-loop region polymorphism.";
RL Anim. Genet. 26:321-326(1995).
RN [12]
RP FUNCTION.
RX PubMed=22690928; DOI=10.1021/ja3001908;
RA Hao G.F., Wang F., Li H., Zhu X.L., Yang W.C., Huang L.S., Wu J.W.,
RA Berry E.A., Yang G.F.;
RT "Computational discovery of picomolar Q(o) site inhibitors of cytochrome
RT bc1 complex.";
RL J. Am. Chem. Soc. 134:11168-11176(2012).
CC -!- FUNCTION: Component of the ubiquinol-cytochrome c reductase complex
CC (complex III or cytochrome b-c1 complex) that is part of the
CC mitochondrial respiratory chain. The b-c1 complex mediates electron
CC transfer from ubiquinol to cytochrome c. Contributes to the generation
CC of a proton gradient across the mitochondrial membrane that is then
CC used for ATP synthesis. {ECO:0000269|PubMed:22690928}.
CC -!- COFACTOR:
CC Name=heme b; Xref=ChEBI:CHEBI:60344;
CC Evidence={ECO:0000250|UniProtKB:P00157};
CC Note=Binds 2 heme b groups non-covalently.
CC {ECO:0000250|UniProtKB:P00157};
CC -!- SUBUNIT: The cytochrome bc1 complex contains 11 subunits: 3 respiratory
CC subunits (MT-CYB, CYC1 and UQCRFS1), 2 core proteins (UQCRC1 and
CC UQCRC2) and 6 low-molecular weight proteins (UQCRH/QCR6, UQCRB/QCR7,
CC UQCRQ/QCR8, UQCR10/QCR9, UQCR11/QCR10 and a cleavage product of
CC UQCRFS1). This cytochrome bc1 complex then forms a dimer.
CC {ECO:0000250|UniProtKB:P00157}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane
CC {ECO:0000250|UniProtKB:P00157}; Multi-pass membrane protein
CC {ECO:0000250|UniProtKB:P00157}.
CC -!- MISCELLANEOUS: Heme 1 (or BL or b562) is low-potential and absorbs at
CC about 562 nm, and heme 2 (or BH or b566) is high-potential and absorbs
CC at about 566 nm. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the cytochrome b family. {ECO:0000255|PROSITE-
CC ProRule:PRU00967, ECO:0000255|PROSITE-ProRule:PRU00968}.
CC -!- CAUTION: The full-length protein contains only eight transmembrane
CC helices, not nine as predicted by bioinformatics tools.
CC {ECO:0000250|UniProtKB:P00157}.
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DR EMBL; X56295; CAA39742.1; -; Genomic_DNA.
DR EMBL; AJ002189; CAA05239.1; -; Genomic_DNA.
DR EMBL; AF034253; AAD34197.1; -; Genomic_DNA.
DR EMBL; Z50079; CAA90410.1; -; Genomic_DNA.
DR EMBL; Z50085; CAA90416.1; -; Genomic_DNA.
DR EMBL; Z50086; CAA90417.1; -; Genomic_DNA.
DR EMBL; Z50087; CAA90418.1; -; Genomic_DNA.
DR EMBL; Z50088; CAA90419.1; -; Genomic_DNA.
DR EMBL; Z50089; CAA90420.1; -; Genomic_DNA.
DR EMBL; AB015065; BAA28876.1; -; Genomic_DNA.
DR EMBL; AB015066; BAA28877.1; -; Genomic_DNA.
DR EMBL; AB015067; BAA28878.1; -; Genomic_DNA.
DR EMBL; AB015068; BAA28879.1; -; Genomic_DNA.
DR EMBL; AB015071; BAA28882.1; -; Genomic_DNA.
DR EMBL; AB015072; BAA28883.1; -; Genomic_DNA.
DR EMBL; AB015073; BAA28884.1; -; Genomic_DNA.
DR EMBL; AB015074; BAA28885.1; -; Genomic_DNA.
DR EMBL; AB015076; BAA28887.1; -; Genomic_DNA.
DR EMBL; AB015079; BAA28890.1; -; Genomic_DNA.
DR EMBL; AB015080; BAA28891.1; -; Genomic_DNA.
DR EMBL; AB015081; BAA28892.1; -; Genomic_DNA.
DR EMBL; AF136541; AAF62354.1; -; Genomic_DNA.
DR EMBL; AF136542; AAF62355.1; -; Genomic_DNA.
DR EMBL; AF136543; AAF62356.1; -; Genomic_DNA.
DR EMBL; AF136544; AAF62357.1; -; Genomic_DNA.
DR EMBL; AF136545; AAF62358.1; -; Genomic_DNA.
DR EMBL; AF136546; AAF62359.1; -; Genomic_DNA.
DR EMBL; AF136547; AAF62360.1; -; Genomic_DNA.
DR EMBL; AF136548; AAF62362.1; -; Genomic_DNA.
DR EMBL; AF136549; AAF62361.1; -; Genomic_DNA.
DR EMBL; AF136550; AAF62363.1; -; Genomic_DNA.
DR EMBL; AF136551; AAF62364.1; -; Genomic_DNA.
DR EMBL; AF136552; AAF62365.1; -; Genomic_DNA.
DR EMBL; AF163099; AAF62368.1; -; Genomic_DNA.
DR EMBL; AF163100; AAF62369.1; -; Genomic_DNA.
DR EMBL; AF304203; AAG28228.1; -; Genomic_DNA.
DR EMBL; AJ314556; CAC85257.1; -; Genomic_DNA.
DR EMBL; AJ314557; CAC85258.1; -; Genomic_DNA.
DR EMBL; AJ314558; CAC85259.1; -; Genomic_DNA.
DR EMBL; AY237484; AAO85585.1; -; Genomic_DNA.
DR EMBL; AY237485; AAO85586.1; -; Genomic_DNA.
DR EMBL; AY237487; AAO85588.1; -; Genomic_DNA.
DR EMBL; AY237488; AAO85589.1; -; Genomic_DNA.
DR EMBL; AY237489; AAO85590.1; -; Genomic_DNA.
DR EMBL; AY237490; AAO85591.1; -; Genomic_DNA.
DR EMBL; AY237491; AAO85592.1; -; Genomic_DNA.
DR EMBL; AY237492; AAO85593.1; -; Genomic_DNA.
DR EMBL; AY237493; AAO85594.1; -; Genomic_DNA.
DR EMBL; AY237494; AAO85595.1; -; Genomic_DNA.
DR EMBL; AY237496; AAO85597.1; -; Genomic_DNA.
DR EMBL; AY237497; AAO85598.1; -; Genomic_DNA.
DR EMBL; AY237498; AAO85599.1; -; Genomic_DNA.
DR EMBL; AY237499; AAO85600.1; -; Genomic_DNA.
DR EMBL; AY237500; AAO85601.1; -; Genomic_DNA.
DR EMBL; AY237501; AAO85602.1; -; Genomic_DNA.
DR EMBL; AY237502; AAO85603.1; -; Genomic_DNA.
DR EMBL; AY237503; AAO85604.1; -; Genomic_DNA.
DR EMBL; AY237504; AAO85605.1; -; Genomic_DNA.
DR EMBL; AY237505; AAO85606.1; -; Genomic_DNA.
DR EMBL; AY237506; AAO85607.1; -; Genomic_DNA.
DR EMBL; AY237507; AAO85608.1; -; Genomic_DNA.
DR EMBL; AY237508; AAO85609.1; -; Genomic_DNA.
DR EMBL; AY237509; AAO85610.1; -; Genomic_DNA.
DR EMBL; AY237510; AAO85611.1; -; Genomic_DNA.
DR EMBL; AY237511; AAO85612.1; -; Genomic_DNA.
DR EMBL; AY237512; AAO85613.1; -; Genomic_DNA.
DR EMBL; AY237513; AAO85614.1; -; Genomic_DNA.
DR EMBL; AY237514; AAO85615.1; -; Genomic_DNA.
DR EMBL; AY237515; AAO85616.1; -; Genomic_DNA.
DR EMBL; AY237517; AAO85618.1; -; Genomic_DNA.
DR EMBL; AY237518; AAO85619.1; -; Genomic_DNA.
DR EMBL; AY237519; AAO85620.1; -; Genomic_DNA.
DR EMBL; AY237520; AAO85621.1; -; Genomic_DNA.
DR EMBL; AY237522; AAO85623.1; -; Genomic_DNA.
DR EMBL; AY237523; AAO85624.1; -; Genomic_DNA.
DR EMBL; AY237526; AAO85627.1; -; Genomic_DNA.
DR EMBL; AY237527; AAO85628.1; -; Genomic_DNA.
DR EMBL; AY237528; AAO85629.1; -; Genomic_DNA.
DR EMBL; AY237529; AAO85630.1; -; Genomic_DNA.
DR EMBL; AY237533; AAO85634.1; -; Genomic_DNA.
DR EMBL; AY237534; AAO85635.1; -; Genomic_DNA.
DR EMBL; AF486858; AAQ06031.1; -; Genomic_DNA.
DR EMBL; AF486866; AAQ06135.1; -; Genomic_DNA.
DR EMBL; AF486869; AAQ06174.1; -; Genomic_DNA.
DR EMBL; D17739; BAA04592.1; -; Genomic_DNA.
DR PIR; T10984; S17418.
DR RefSeq; NP_008646.1; NC_000845.1.
DR AlphaFoldDB; P24964; -.
DR SMR; P24964; -.
DR STRING; 9823.ENSSSCP00000019147; -.
DR BindingDB; P24964; -.
DR ChEMBL; CHEMBL2366425; -.
DR PaxDb; P24964; -.
DR PeptideAtlas; P24964; -.
DR GeneID; 808513; -.
DR KEGG; ssc:808513; -.
DR CTD; 4519; -.
DR eggNOG; KOG4663; Eukaryota.
DR InParanoid; P24964; -.
DR OrthoDB; 1125966at2759; -.
DR ChiTaRS; CYTB; pig.
DR PRO; PR:P24964; -.
DR Proteomes; UP000008227; Mitochondrion.
DR Proteomes; UP000314985; Mitochondrion.
DR GO; GO:0005743; C:mitochondrial inner membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0045275; C:respiratory chain complex III; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0008121; F:ubiquinol-cytochrome-c reductase activity; IDA:UniProtKB.
DR GO; GO:0006122; P:mitochondrial electron transport, ubiquinol to cytochrome c; IDA:UniProtKB.
DR CDD; cd00290; cytochrome_b_C; 1.
DR CDD; cd00284; Cytochrome_b_N; 1.
DR Gene3D; 1.20.810.10; -; 1.
DR InterPro; IPR005798; Cyt_b/b6_C.
DR InterPro; IPR036150; Cyt_b/b6_C_sf.
DR InterPro; IPR005797; Cyt_b/b6_N.
DR InterPro; IPR027387; Cytb/b6-like_sf.
DR InterPro; IPR030689; Cytochrome_b.
DR InterPro; IPR016174; Di-haem_cyt_TM.
DR Pfam; PF00032; Cytochrom_B_C; 1.
DR Pfam; PF00033; Cytochrome_B; 1.
DR PIRSF; PIRSF038885; COB; 1.
DR SUPFAM; SSF81342; SSF81342; 1.
DR SUPFAM; SSF81648; SSF81648; 1.
DR PROSITE; PS51003; CYTB_CTER; 1.
DR PROSITE; PS51002; CYTB_NTER; 1.
PE 3: Inferred from homology;
KW Electron transport; Heme; Iron; Membrane; Metal-binding; Mitochondrion;
KW Mitochondrion inner membrane; Reference proteome; Respiratory chain;
KW Transmembrane; Transmembrane helix; Transport; Ubiquinone.
FT CHAIN 1..379
FT /note="Cytochrome b"
FT /id="PRO_0000061397"
FT TRANSMEM 33..53
FT /note="Helical"
FT /evidence="ECO:0000250|UniProtKB:P00157"
FT TRANSMEM 77..98
FT /note="Helical"
FT /evidence="ECO:0000250|UniProtKB:P00157"
FT TRANSMEM 113..133
FT /note="Helical"
FT /evidence="ECO:0000250|UniProtKB:P00157"
FT TRANSMEM 178..198
FT /note="Helical"
FT /evidence="ECO:0000250|UniProtKB:P00157"
FT TRANSMEM 226..246
FT /note="Helical"
FT /evidence="ECO:0000250|UniProtKB:P00157"
FT TRANSMEM 288..308
FT /note="Helical"
FT /evidence="ECO:0000250|UniProtKB:P00157"
FT TRANSMEM 320..340
FT /note="Helical"
FT /evidence="ECO:0000250|UniProtKB:P00157"
FT TRANSMEM 347..367
FT /note="Helical"
FT /evidence="ECO:0000250|UniProtKB:P00157"
FT BINDING 83
FT /ligand="heme b"
FT /ligand_id="ChEBI:CHEBI:60344"
FT /ligand_label="b562"
FT /ligand_part="Fe"
FT /ligand_part_id="ChEBI:CHEBI:18248"
FT /note="axial binding residue"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00968"
FT BINDING 97
FT /ligand="heme b"
FT /ligand_id="ChEBI:CHEBI:60344"
FT /ligand_label="b566"
FT /ligand_part="Fe"
FT /ligand_part_id="ChEBI:CHEBI:18248"
FT /note="axial binding residue"
FT /evidence="ECO:0000250|UniProtKB:P00157"
FT BINDING 182
FT /ligand="heme b"
FT /ligand_id="ChEBI:CHEBI:60344"
FT /ligand_label="b562"
FT /ligand_part="Fe"
FT /ligand_part_id="ChEBI:CHEBI:18248"
FT /note="axial binding residue"
FT /evidence="ECO:0000250|UniProtKB:P00157"
FT BINDING 196
FT /ligand="heme b"
FT /ligand_id="ChEBI:CHEBI:60344"
FT /ligand_label="b566"
FT /ligand_part="Fe"
FT /ligand_part_id="ChEBI:CHEBI:18248"
FT /note="axial binding residue"
FT /evidence="ECO:0000250|UniProtKB:P00157"
FT BINDING 201
FT /ligand="a ubiquinone"
FT /ligand_id="ChEBI:CHEBI:16389"
FT /evidence="ECO:0000250|UniProtKB:P00157"
FT VARIANT 123
FT /note="V -> I (in strain: Ryukyu wild boar 6)"
FT VARIANT 184
FT /note="I -> T (in strain: Ryukyu wild boar 6)"
FT VARIANT 215
FT /note="M -> I (in strain: Japanese wild boar 4, Japanese
FT wild boar 11 and AWB10)"
FT VARIANT 295
FT /note="V -> M (in strain: Bulgarian 2 and Meishan)"
FT VARIANT 314
FT /note="G -> S (in strain: Asian domestic pig, Landrace,
FT Chinese Diannan short-ear, Large white 1, Japanese wild
FT boar, P2, Ryukyu wild boar, Satsumae 28, Seishan, Ssc2,
FT AWB10, AWB11, EWB3 and LW1)"
FT VARIANT 368
FT /note="I -> V (in strain: IB9, IB14, IB16, Sardinian wild
FT boar 2 and Ssc3)"
FT CONFLICT 198
FT /note="L -> M (in Ref. 1; CAA39742)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 379 AA; 42782 MW; 32F4428CF9CD848E CRC64;
MTNIRKSHPL MKIINNAFID LPAPSNISSW WNFGSLLGIC LILQILTGLF LAMHYTSDTT
TAFSSVTHIC RDVNYGWVIR YLHANGASMF FICLFIHVGR GLYYGSYMFL ETWNIGVVLL
FTVMATAFMG YVLPWGQMSF WGATVITNLL SAIPYIGTDL VEWIWGGFSV DKATLTRFFA
FHFILPFIIT ALAAVHLLFL HETGSNNPTG ISSDMDKIPF HPYYTIKDIL GALFMMLILL
ILVLFSPDLL GDPDNYTPAN PLNTPPHIKP EWYFLFAYAI LRSIPNKLGG VLALVASILI
LILMPMLHTS KQRGMMFRPL SQCLFWMLVA DLITLTWIGG QPVEHPFIII GQLASILYFL
IILVLMPITS IIENNLLKW
