ID   ACR_MYCBO               Reviewed;         144 AA.
AC   P0A5B8; A0A1R3Y251; A7VJB3; P30223; X2BJK6;
DT   15-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 2.
DT   25-MAY-2022, entry version 84.
DE   RecName: Full=Alpha-crystallin {ECO:0000303|PubMed:9766227};
DE            Short=Acr;
DE   AltName: Full=14 kDa antigen;
DE   AltName: Full=16 kDa antigen;
DE   AltName: Full=HSP 16.3;
GN   Name=hspX; Synonyms=acr; OrderedLocusNames=BQ2027_MB2057C;
OS   Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=233413;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], SUBCELLULAR LOCATION, AND INDUCTION.
RC   STRAIN=BCG / Tokyo;
RX   PubMed=9766227; DOI=10.1016/s0923-2508(98)80301-x;
RA   Tabira Y., Ohara N., Kitaura H., Matsumoto S., Naito M., Yamada T.;
RT   "The 16-kDa alpha-crystallin-like protein of Mycobacterium bovis BCG is
RT   produced under conditions of oxygen deficiency and is associated with
RT   ribosomes.";
RL   Res. Microbiol. 149:255-264(1998).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-935 / AF2122/97;
RX   PubMed=12788972; DOI=10.1073/pnas.1130426100;
RA   Garnier T., Eiglmeier K., Camus J.-C., Medina N., Mansoor H., Pryor M.,
RA   Duthoy S., Grondin S., Lacroix C., Monsempe C., Simon S., Harris B.,
RA   Atkin R., Doggett J., Mayes R., Keating L., Wheeler P.R., Parkhill J.,
RA   Barrell B.G., Cole S.T., Gordon S.V., Hewinson R.G.;
RT   "The complete genome sequence of Mycobacterium bovis.";
RL   Proc. Natl. Acad. Sci. U.S.A. 100:7877-7882(2003).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND GENOME REANNOTATION.
RC   STRAIN=ATCC BAA-935 / AF2122/97;
RX   PubMed=28385856; DOI=10.1128/genomea.00157-17;
RA   Malone K.M., Farrell D., Stuber T.P., Schubert O.T., Aebersold R.,
RA   Robbe-Austerman S., Gordon S.V.;
RT   "Updated reference genome sequence and annotation of Mycobacterium bovis
RT   AF2122/97.";
RL   Genome Announc. 5:E00157-E00157(2017).
RN   [4]
RP   PROTEIN SEQUENCE OF 2-11, AND SUBCELLULAR LOCATION.
RC   STRAIN=BCG / Tokyo;
RX   PubMed=8359926; DOI=10.1128/iai.61.9.4005-4007.1993;
RA   Tantimavanich S., Nagai S., Nomaguchi H., Kinomoto M., Ohara N., Yamada T.;
RT   "Immunological properties of ribosomal proteins from Mycobacterium bovis
RT   BCG.";
RL   Infect. Immun. 61:4005-4007(1993).
CC   -!- FUNCTION: Acts as a chaperone. {ECO:0000250|UniProtKB:P9WMK1}.
CC   -!- SUBCELLULAR LOCATION: Secreted, cell wall {ECO:0000269|PubMed:9766227}.
CC       Cytoplasm {ECO:0000269|PubMed:8359926, ECO:0000269|PubMed:9766227}.
CC       Note=Associates tightly with 30S ribosome subunits (PubMed:8359926,
CC       PubMed:9766227). Probably on the external side of the cell wall (By
CC       similarity). {ECO:0000250|UniProtKB:P9WMK1, ECO:0000269|PubMed:8359926,
CC       ECO:0000269|PubMed:9766227}.
CC   -!- INDUCTION: Present in cells grown on plates but not in those grown in
CC       shaken liquid culture (at protein level). Induced in the absence of
CC       oxygen (hypoxia) (at protein level). {ECO:0000269|PubMed:9766227}.
CC   -!- SIMILARITY: Belongs to the small heat shock protein (HSP20) family.
CC       {ECO:0000255|PROSITE-ProRule:PRU00285}.
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DR   EMBL; AB005789; BAF79634.1; -; Genomic_DNA.
DR   EMBL; LT708304; SIU00664.1; -; Genomic_DNA.
DR   RefSeq; NP_855707.1; NC_002945.3.
DR   RefSeq; WP_003410189.1; NC_002945.4.
DR   AlphaFoldDB; P0A5B8; -.
DR   SMR; P0A5B8; -.
DR   EnsemblBacteria; SIU00664; SIU00664; BQ2027_MB2057C.
DR   PATRIC; fig|1765.299.peg.2266; -.
DR   OMA; AGYDKGI; -.
DR   Proteomes; UP000001419; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-KW.
DR   Gene3D; 2.60.40.790; -; 1.
DR   InterPro; IPR002068; A-crystallin/Hsp20_dom.
DR   InterPro; IPR008978; HSP20-like_chaperone.
DR   Pfam; PF00011; HSP20; 1.
DR   SUPFAM; SSF49764; SSF49764; 1.
DR   PROSITE; PS01031; SHSP; 1.
PE   1: Evidence at protein level;
KW   Cell wall; Chaperone; Cytoplasm; Direct protein sequencing; Secreted.
FT   INIT_MET        1
FT                   /note="Removed"
FT                   /evidence="ECO:0000269|PubMed:8359926"
FT   CHAIN           2..144
FT                   /note="Alpha-crystallin"
FT                   /id="PRO_0000126010"
FT   DOMAIN          33..143
FT                   /note="sHSP"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00285"
FT   CONFLICT        9..10
FT                   /note="RH -> D (in Ref. 4; AA sequence)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   144 AA;  16227 MW;  A0C96872035CDDF8 CRC64;
     MATTLPVQRH PRSLFPEFSE LFAAFPSFAG LRPTFDTRLM RLEDEMKEGR YEVRAELPGV
     DPDKDVDIMV RDGQLTIKAE RTEQKDFDGR SEFAYGSFVR TVSLPVGADE DDIKATYDKG
     ILTVSVAVSE GKPTEKHIQI RSTN