ACSA1_ARATH
ID ACSA1_ARATH Reviewed; 450 AA.
AC Q93ZG3; Q680F0; Q9C6N4;
DT 28-MAR-2018, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 03-AUG-2022, entry version 157.
DE RecName: Full=WD repeat-containing protein ATCSA-1 {ECO:0000305};
DE AltName: Full=Cockayne syndrome WD repeat protein CSA homolog {ECO:0000305};
GN Name=ATCSA-1 {ECO:0000303|PubMed:20128879};
GN OrderedLocusNames=At1g27840 {ECO:0000312|Araport:AT1G27840};
GN ORFNames=F28L5.15 {ECO:0000312|EMBL:AAG50581.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP FUNCTION, INTERACTION WITH DDB1A, SUBCELLULAR LOCATION, TISSUE SPECIFICITY,
RP AND DISRUPTION PHENOTYPE.
RX PubMed=20128879; DOI=10.1111/j.1365-313x.2010.04157.x;
RA Biedermann S., Hellmann H.;
RT "The DDB1a interacting proteins ATCSA-1 and DDB2 are critical factors for
RT UV-B tolerance and genomic integrity in Arabidopsis thaliana.";
RL Plant J. 62:404-415(2010).
CC -!- FUNCTION: Involved in UV-B tolerance and genome integrity. In
CC association with DDB2, is necessary for repair of UV-B-induced DNA
CC lesions. {ECO:0000269|PubMed:20128879}.
CC -!- SUBUNIT: Interacts with DDB1A. {ECO:0000269|PubMed:20128879}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:20128879}.
CC Note=Localizes in a speckled pattern. {ECO:0000269|PubMed:20128879}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q93ZG3-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q93ZG3-2; Sequence=VSP_059390;
CC -!- TISSUE SPECIFICITY: Expressed in roots, leaves, stems, flowers and
CC siliques. {ECO:0000269|PubMed:20128879}.
CC -!- DISRUPTION PHENOTYPE: No visible phenotype under normal growth
CC conditions, but mutant plants exhibit sensitivity to UV-B and decreased
CC DNA repair activity following UV-B treatment.
CC {ECO:0000269|PubMed:20128879}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAG50581.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC079280; AAG50581.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE30881.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE30882.1; -; Genomic_DNA.
DR EMBL; AY057556; AAL09795.1; -; mRNA.
DR EMBL; AY074265; AAL66962.1; -; mRNA.
DR EMBL; AY096624; AAM20274.1; -; mRNA.
DR EMBL; AK175747; BAD43510.1; -; mRNA.
DR EMBL; AK176142; BAD43905.1; -; mRNA.
DR EMBL; AK175917; BAD43680.1; -; mRNA.
DR PIR; E86403; E86403.
DR RefSeq; NP_001031098.1; NM_001036021.1. [Q93ZG3-2]
DR RefSeq; NP_174105.2; NM_102549.5. [Q93ZG3-1]
DR AlphaFoldDB; Q93ZG3; -.
DR SMR; Q93ZG3; -.
DR STRING; 3702.AT1G27840.3; -.
DR PRIDE; Q93ZG3; -.
DR ProteomicsDB; 244353; -. [Q93ZG3-1]
DR EnsemblPlants; AT1G27840.1; AT1G27840.1; AT1G27840. [Q93ZG3-1]
DR EnsemblPlants; AT1G27840.2; AT1G27840.2; AT1G27840. [Q93ZG3-2]
DR GeneID; 839677; -.
DR Gramene; AT1G27840.1; AT1G27840.1; AT1G27840. [Q93ZG3-1]
DR Gramene; AT1G27840.2; AT1G27840.2; AT1G27840. [Q93ZG3-2]
DR KEGG; ath:AT1G27840; -.
DR Araport; AT1G27840; -.
DR OMA; MTAVKAF; -.
DR PhylomeDB; Q93ZG3; -.
DR PRO; PR:Q93ZG3; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q93ZG3; baseline and differential.
DR GO; GO:0031464; C:Cul4A-RING E3 ubiquitin ligase complex; IBA:GO_Central.
DR GO; GO:0000109; C:nucleotide-excision repair complex; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0006281; P:DNA repair; IMP:UniProtKB.
DR GO; GO:0043161; P:proteasome-mediated ubiquitin-dependent protein catabolic process; IBA:GO_Central.
DR GO; GO:0000209; P:protein polyubiquitination; IBA:GO_Central.
DR GO; GO:0010224; P:response to UV-B; IMP:UniProtKB.
DR GO; GO:0006283; P:transcription-coupled nucleotide-excision repair; IBA:GO_Central.
DR Gene3D; 2.130.10.10; -; 1.
DR InterPro; IPR020472; G-protein_beta_WD-40_rep.
DR InterPro; IPR042238; Rad28/ERCC8/Ckn1/ATCSA-1.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR019775; WD40_repeat_CS.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR PANTHER; PTHR46202; PTHR46202; 1.
DR Pfam; PF00400; WD40; 4.
DR PRINTS; PR00320; GPROTEINBRPT.
DR SMART; SM00320; WD40; 5.
DR SUPFAM; SSF50978; SSF50978; 1.
DR PROSITE; PS00678; WD_REPEATS_1; 2.
DR PROSITE; PS50082; WD_REPEATS_2; 4.
DR PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; DNA damage; DNA repair; Nucleus; Reference proteome;
KW Repeat; WD repeat.
FT CHAIN 1..450
FT /note="WD repeat-containing protein ATCSA-1"
FT /id="PRO_0000443536"
FT REPEAT 41..81
FT /note="WD 1"
FT /evidence="ECO:0000255"
FT REPEAT 101..141
FT /note="WD 2"
FT /evidence="ECO:0000255"
FT REPEAT 148..185
FT /note="WD 3"
FT /evidence="ECO:0000255"
FT REPEAT 188..228
FT /note="WD 4"
FT /evidence="ECO:0000255"
FT REPEAT 310..349
FT /note="WD 5"
FT /evidence="ECO:0000255"
FT REPEAT 397..436
FT /note="WD 6"
FT /evidence="ECO:0000255"
FT REGION 269..298
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 269..288
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 81..137
FT /note="Missing (in isoform 2)"
FT /id="VSP_059390"
SQ SEQUENCE 450 AA; 49654 MW; 357DFC34EDEE988C CRC64;
MWEAIKDRET GRIRSNSFAN RFKSRRILSL QLSNRKDFVS PHRGSVNSLQ VDLTEGRYLL
SGAADGSAAV FDVQRATDYE ASGLIAKHKC IFTVDKQHEN GHKYAISSAI WYPIDTGLFI
TGSFDHYLKV WDTNTAQAVV DFKMPGKVYR TAMSSMAMSH TLIAAGTEDV QVRLCDIASG
AFSHTLSGHR DGVMSVEWST SSEWVLYTGG CDGAIRFWDI RRAGCFRVLD QSQTQLGFRP
PILKRTAVGS KLSSVAKSSL GGQNRLKTLQ SKQTGSQSVK GSSSAKASVE KSRQKRIHPG
MLSTLDRATA HYGAVTGLKA TNDGMYLLSA GSDSRIRLWD IESGRNTLVN FETGRIQTNK
GIQLDTSDDP ALVFVPCMKT VKAFGMWSGR TTLMLRGHYE SVNTCCFNSN DQELYTSGSD
RQILVWSPGG TVEDEMVQDE VAEDKDNWSD
