ID   CYC13_CATRO             Reviewed;         507 AA.
AC   W8JIS9;
DT   10-APR-2019, integrated into UniProtKB/Swiss-Prot.
DT   14-MAY-2014, sequence version 1.
DT   03-AUG-2022, entry version 16.
DE   RecName: Full=Cytochrome P450 81C13 {ECO:0000303|PubMed:24710322};
DE            Short=CrCYP81C13 {ECO:0000303|PubMed:24710322};
DE            EC=1.14.-.- {ECO:0000305};
DE   Flags: Precursor;
GN   Name=CYP81C13 {ECO:0000303|PubMed:24710322};
GN   ORFNames=Caros008992 {ECO:0000305};
OS   Catharanthus roseus (Madagascar periwinkle) (Vinca rosea).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; lamiids; Gentianales; Apocynaceae; Rauvolfioideae; Vinceae;
OC   Catharanthinae; Catharanthus.
OX   NCBI_TaxID=4058;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. Little Bright Eyes;
RX   PubMed=24710322; DOI=10.1038/ncomms4606;
RA   Miettinen K., Dong L., Navrot N., Schneider T., Burlat V., Pollier J.,
RA   Woittiez L., van der Krol S., Lugan R., Ilc T., Verpoorte R.,
RA   Oksman-Caldentey K.M., Martinoia E., Bouwmeester H., Goossens A.,
RA   Memelink J., Werck-Reichhart D.;
RT   "The seco-iridoid pathway from Catharanthus roseus.";
RL   Nat. Commun. 5:3606-3606(2014).
CC   -!- SIMILARITY: Belongs to the cytochrome P450 family. {ECO:0000305}.
CC   -!- WEB RESOURCE: Name=ORCAE database;
CC       URL="https://bioinformatics.psb.ugent.be/orcae/overview/Catro";
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DR   EMBL; KF302071; AHK60838.1; -; mRNA.
DR   AlphaFoldDB; W8JIS9; -.
DR   SMR; W8JIS9; -.
DR   GO; GO:0020037; F:heme binding; IEA:InterPro.
DR   GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR   GO; GO:0004497; F:monooxygenase activity; IEA:UniProtKB-KW.
DR   GO; GO:0016705; F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen; IEA:InterPro.
DR   Gene3D; 1.10.630.10; -; 1.
DR   InterPro; IPR001128; Cyt_P450.
DR   InterPro; IPR017972; Cyt_P450_CS.
DR   InterPro; IPR002401; Cyt_P450_E_grp-I.
DR   InterPro; IPR036396; Cyt_P450_sf.
DR   Pfam; PF00067; p450; 1.
DR   PRINTS; PR00463; EP450I.
DR   PRINTS; PR00385; P450.
DR   SUPFAM; SSF48264; SSF48264; 1.
DR   PROSITE; PS00086; CYTOCHROME_P450; 1.
PE   2: Evidence at transcript level;
KW   Glycoprotein; Heme; Iron; Metal-binding; Monooxygenase; Oxidoreductase;
KW   Signal.
FT   SIGNAL          1..22
FT                   /evidence="ECO:0000255"
FT   CHAIN           23..507
FT                   /note="Cytochrome P450 81C13"
FT                   /id="PRO_0000446411"
FT   BINDING         443
FT                   /ligand="heme"
FT                   /ligand_id="ChEBI:CHEBI:30413"
FT                   /ligand_part="Fe"
FT                   /ligand_part_id="ChEBI:CHEBI:18248"
FT                   /note="axial binding residue"
FT                   /evidence="ECO:0000250|UniProtKB:P04798"
FT   CARBOHYD        347
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT   CARBOHYD        362
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT   CARBOHYD        475
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
SQ   SEQUENCE   507 AA;  58975 MW;  9BF14F1BD755C8C5 CRC64;
     MANFISFFIL FSALFFLFKR LFFPNRKQKL PPGPPSLPIV GHLHHIKTSL YQALARLSNE
     YGPVIFLRFG CRSFVAVSSP SAIEECFTKN DIIFANRPKS MATDRLTFNY IGFAWAPYGQ
     FWRIVRRFTV VEFFSTNCLG KSRPIREEEI QSILRSIFKI TKMKSEKIDL KHWVSVFTFN
     VLMRTMADKW AVDEEDSGVE KGKEIMKELR EIFFTNVAMN VCDFFPIFRW FGYKGIEQRM
     INLHNRRNEF LQGLVDKVRI NKKSGFVRNK ESHRSLIEAF LSHQDQDPEF FTDDLIKSVL
     LIMFVAGTET SAVIIEWAMS LLLSNPDVLN KLRIEIDKQI GNKRLINESD IPKLPYLRCI
     INETMRLFPP VPLLLPHCSS EDCKIAGYDI PKGTVLFVNA WATHRDPKLW DEPEKFIPER
     FENMEVEKEG YKFIPFGIGR RACPGTSMGI RTVSLALGAF IQCFDWKKVE EYELNTSYDY
     RITLQKANQL EASCSPRQDF FPILSQL