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CYC1_STRCO
ID   CYC1_STRCO              Reviewed;         361 AA.
AC   Q9K499;
DT   25-JUL-2006, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2000, sequence version 1.
DT   03-AUG-2022, entry version 111.
DE   RecName: Full=Epi-isozizaene synthase;
DE            Short=EIZS;
DE            EC=4.2.3.37;
DE   AltName: Full=Sesquiterpene cyclase;
DE   AltName: Full=Sesquiterpene synthase;
GN   Name=cyc1; OrderedLocusNames=SCO5222; ORFNames=SC7E4.19;
OS   Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145).
OC   Bacteria; Actinobacteria; Streptomycetales; Streptomycetaceae;
OC   Streptomyces; Streptomyces albidoflavus group.
OX   NCBI_TaxID=100226;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-471 / A3(2) / M145;
RX   PubMed=12000953; DOI=10.1038/417141a;
RA   Bentley S.D., Chater K.F., Cerdeno-Tarraga A.-M., Challis G.L.,
RA   Thomson N.R., James K.D., Harris D.E., Quail M.A., Kieser H., Harper D.,
RA   Bateman A., Brown S., Chandra G., Chen C.W., Collins M., Cronin A.,
RA   Fraser A., Goble A., Hidalgo J., Hornsby T., Howarth S., Huang C.-H.,
RA   Kieser T., Larke L., Murphy L.D., Oliver K., O'Neil S., Rabbinowitsch E.,
RA   Rajandream M.A., Rutherford K.M., Rutter S., Seeger K., Saunders D.,
RA   Sharp S., Squares R., Squares S., Taylor K., Warren T., Wietzorrek A.,
RA   Woodward J.R., Barrell B.G., Parkhill J., Hopwood D.A.;
RT   "Complete genome sequence of the model actinomycete Streptomyces coelicolor
RT   A3(2).";
RL   Nature 417:141-147(2002).
RN   [2]
RP   FUNCTION, COFACTOR, KINETIC PARAMETERS, REACTION MECHANISM, AND REACTION
RP   STEREOCHEMISTRY.
RC   STRAIN=ATCC BAA-471 / A3(2) / M145;
RX   PubMed=16669656; DOI=10.1021/ja061292s;
RA   Lin X., Hopson R., Cane D.E.;
RT   "Genome mining in Streptomyces coelicolor. Molecular cloning and
RT   characterization of a new sesquiterpene synthase.";
RL   J. Am. Chem. Soc. 128:6022-6023(2006).
CC   -!- FUNCTION: Catalyzes the cyclization of farnesyl diphosphate (FPP) to
CC       the sesquiterpene epi-isozizaene. {ECO:0000269|PubMed:16669656}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(2E,6E)-farnesyl diphosphate = (+)-epi-isozizaene +
CC         diphosphate; Xref=Rhea:RHEA:25992, ChEBI:CHEBI:33019,
CC         ChEBI:CHEBI:51458, ChEBI:CHEBI:175763; EC=4.2.3.37;
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC         Evidence={ECO:0000269|PubMed:16669656};
CC       Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
CC         Evidence={ECO:0000269|PubMed:16669656};
CC       Name=Fe(3+); Xref=ChEBI:CHEBI:29034;
CC         Evidence={ECO:0000269|PubMed:16669656};
CC       Note=Binds 3 Mg(2+) ions per subunit. Can also use Mn(2+) and Fe(3+)
CC       but less efficiently, and other divalent cations such as Zn(2+),
CC       Fe(2+), Co(2+), Cu(2+) and Ni(2+) are nearly completely inefficient as
CC       cofactors. {ECO:0000269|PubMed:16669656};
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       Kinetic parameters:
CC         KM=147 nM for FPP {ECO:0000269|PubMed:16669656};
CC   -!- PATHWAY: Sesquiterpene biosynthesis; epi-isozizaene biosynthesis.
CC   -!- DOMAIN: The Asp-Asp-Xaa-Xaa-Asp/Glu (DDXXD/E) motif is important for
CC       the catalytic activity, presumably through binding to Mg(2+).
CC   -!- SIMILARITY: Belongs to the terpene synthase family. {ECO:0000305}.
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DR   EMBL; AL939123; CAB94607.1; -; Genomic_DNA.
DR   RefSeq; NP_629369.1; NC_003888.3.
DR   RefSeq; WP_011030119.1; NZ_VNID01000008.1.
DR   PDB; 3KB9; X-ray; 1.60 A; A=2-361.
DR   PDB; 3KBK; X-ray; 1.90 A; A=2-361.
DR   PDB; 3LG5; X-ray; 1.64 A; A=1-361.
DR   PDB; 3LGK; X-ray; 1.89 A; A=2-361.
DR   PDB; 4LTV; X-ray; 2.40 A; A=2-361.
DR   PDB; 4LTZ; X-ray; 2.45 A; A=2-361.
DR   PDB; 4LUU; X-ray; 1.95 A; A=2-361.
DR   PDB; 4LXW; X-ray; 2.09 A; A=2-361.
DR   PDB; 4LZ0; X-ray; 1.75 A; A=2-361.
DR   PDB; 4LZ3; X-ray; 2.10 A; A=2-361.
DR   PDB; 4LZC; X-ray; 2.46 A; A=2-361.
DR   PDB; 6AX9; X-ray; 2.40 A; A=2-361.
DR   PDB; 6AXM; X-ray; 1.80 A; A=2-361.
DR   PDB; 6AXN; X-ray; 1.90 A; A=2-361.
DR   PDB; 6AXO; X-ray; 2.10 A; A=2-361.
DR   PDB; 6AXU; X-ray; 1.82 A; A=2-361.
DR   PDB; 6OFV; X-ray; 1.91 A; A=1-361.
DR   PDB; 7KJ8; X-ray; 1.90 A; A=2-361.
DR   PDB; 7KJ9; X-ray; 2.20 A; A=2-361.
DR   PDB; 7KJD; X-ray; 1.50 A; A=2-361.
DR   PDB; 7KJE; X-ray; 1.60 A; A=2-361.
DR   PDB; 7KJF; X-ray; 1.40 A; A=2-361.
DR   PDB; 7KJG; X-ray; 1.30 A; A=2-361.
DR   PDBsum; 3KB9; -.
DR   PDBsum; 3KBK; -.
DR   PDBsum; 3LG5; -.
DR   PDBsum; 3LGK; -.
DR   PDBsum; 4LTV; -.
DR   PDBsum; 4LTZ; -.
DR   PDBsum; 4LUU; -.
DR   PDBsum; 4LXW; -.
DR   PDBsum; 4LZ0; -.
DR   PDBsum; 4LZ3; -.
DR   PDBsum; 4LZC; -.
DR   PDBsum; 6AX9; -.
DR   PDBsum; 6AXM; -.
DR   PDBsum; 6AXN; -.
DR   PDBsum; 6AXO; -.
DR   PDBsum; 6AXU; -.
DR   PDBsum; 6OFV; -.
DR   PDBsum; 7KJ8; -.
DR   PDBsum; 7KJ9; -.
DR   PDBsum; 7KJD; -.
DR   PDBsum; 7KJE; -.
DR   PDBsum; 7KJF; -.
DR   PDBsum; 7KJG; -.
DR   AlphaFoldDB; Q9K499; -.
DR   SMR; Q9K499; -.
DR   STRING; 100226.SCO5222; -.
DR   GeneID; 1100663; -.
DR   KEGG; sco:SCO5222; -.
DR   PATRIC; fig|100226.15.peg.5306; -.
DR   eggNOG; ENOG5033S8Y; Bacteria.
DR   HOGENOM; CLU_042538_4_1_11; -.
DR   InParanoid; Q9K499; -.
DR   OMA; DLIEYAM; -.
DR   PhylomeDB; Q9K499; -.
DR   BioCyc; MetaCyc:MON-13904; -.
DR   BRENDA; 4.2.3.37; 5998.
DR   SABIO-RK; Q9K499; -.
DR   UniPathway; UPA00195; -.
DR   EvolutionaryTrace; Q9K499; -.
DR   Proteomes; UP000001973; Chromosome.
DR   GO; GO:0052680; F:epi-isozizaene synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.600.10; -; 1.
DR   InterPro; IPR008949; Isoprenoid_synthase_dom_sf.
DR   InterPro; IPR034686; Terpene_cyclase-like_2.
DR   SFLD; SFLDG01020; Terpene_Cyclase_Like_2; 1.
DR   SUPFAM; SSF48576; SSF48576; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Lyase; Magnesium; Metal-binding; Reference proteome.
FT   CHAIN           1..361
FT                   /note="Epi-isozizaene synthase"
FT                   /id="PRO_0000247894"
FT   MOTIF           99..103
FT                   /note="DDXXD motif"
FT   BINDING         99
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_label="1"
FT   BINDING         99
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_label="2"
FT   BINDING         103
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_label="1"
FT   BINDING         103
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_label="2"
FT   BINDING         240
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_label="3"
FT   BINDING         244
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_label="3"
FT   BINDING         248
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_label="3"
FT   TURN            19..21
FT                   /evidence="ECO:0007829|PDB:7KJG"
FT   HELIX           24..29
FT                   /evidence="ECO:0007829|PDB:7KJG"
FT   HELIX           38..51
FT                   /evidence="ECO:0007829|PDB:7KJG"
FT   HELIX           57..67
FT                   /evidence="ECO:0007829|PDB:7KJG"
FT   HELIX           69..74
FT                   /evidence="ECO:0007829|PDB:7KJG"
FT   HELIX           82..107
FT                   /evidence="ECO:0007829|PDB:7KJG"
FT   HELIX           111..126
FT                   /evidence="ECO:0007829|PDB:7KJG"
FT   HELIX           129..131
FT                   /evidence="ECO:0007829|PDB:7KJG"
FT   HELIX           137..149
FT                   /evidence="ECO:0007829|PDB:7KJG"
FT   HELIX           155..181
FT                   /evidence="ECO:0007829|PDB:7KJG"
FT   HELIX           187..197
FT                   /evidence="ECO:0007829|PDB:7KJG"
FT   HELIX           200..211
FT                   /evidence="ECO:0007829|PDB:7KJG"
FT   HELIX           217..220
FT                   /evidence="ECO:0007829|PDB:7KJG"
FT   HELIX           223..250
FT                   /evidence="ECO:0007829|PDB:7KJG"
FT   STRAND          251..254
FT                   /evidence="ECO:0007829|PDB:3LGK"
FT   HELIX           257..265
FT                   /evidence="ECO:0007829|PDB:7KJG"
FT   HELIX           269..301
FT                   /evidence="ECO:0007829|PDB:7KJG"
FT   STRAND          303..305
FT                   /evidence="ECO:0007829|PDB:7KJD"
FT   HELIX           306..335
FT                   /evidence="ECO:0007829|PDB:7KJG"
FT   HELIX           337..339
FT                   /evidence="ECO:0007829|PDB:7KJG"
FT   HELIX           341..343
FT                   /evidence="ECO:0007829|PDB:7KJG"
SQ   SEQUENCE   361 AA;  41388 MW;  227B08D792F36BB7 CRC64;
     MHAFPHGTTA TPTAIAVPPS LRLPVIEAAF PRQLHPYWPK LQETTRTWLL EKRLMPADKV
     EEYADGLCYT DLMAGYYLGA PDEVLQAIAD YSAWFFVWDD RHDRDIVHGR AGAWRRLRGL
     LHTALDSPGD HLHHEDTLVA GFADSVRRLY AFLPATWNAR FARHFHTVIE AYDREFHNRT
     RGIVPGVEEY LELRRLTFAH WIWTDLLEPS SGCELPDAVR KHPAYRRAAL LSQEFAAWYN
     DLCSLPKEIA GDEVHNLGIS LITHHSLTLE EAIGEVRRRV EECITEFLAV ERDALRFADE
     LADGTVRGKE LSGAVRANVG NMRNWFSSVY WFHHESGRYM VDSWDDRSTP PYVNNEAAGE
     K
 
 
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