ID   CYC21_CLITE             Reviewed;          28 AA.
AC   C0HJS8;
DT   08-JUN-2016, integrated into UniProtKB/Swiss-Prot.
DT   08-JUN-2016, sequence version 1.
DT   25-MAY-2022, entry version 8.
DE   RecName: Full=Cliotide T21 {ECO:0000303|PubMed:27007913};
DE   AltName: Full=Cyclotide cT21 {ECO:0000303|PubMed:27007913};
OS   Clitoria ternatea (Butterfly pea).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC   NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Clitoria.
OX   NCBI_TaxID=43366 {ECO:0000303|PubMed:27007913};
RN   [1] {ECO:0000305}
RP   PROTEIN SEQUENCE, FUNCTION, TISSUE SPECIFICITY, CYCLIZATION, PRESENCE OF
RP   DISULFIDE BONDS, MASS SPECTROMETRY, AND IDENTIFICATION BY MASS
RP   SPECTROMETRY.
RX   PubMed=27007913; DOI=10.1111/febs.13720;
RA   Nguyen K.N., Nguyen G.K., Nguyen P.Q., Ang K.H., Dedon P.C., Tam J.P.;
RT   "Immunostimulating and Gram-negative-specific antibacterial cyclotides from
RT   the butterfly pea Clitoria ternatea.";
RL   FEBS J. 283:2067-2090(2016).
CC   -!- FUNCTION: Probably participates in a plant defense mechanism. Not
CC       active against Gram-negative bacterium E.coli ATCC 700926 or Gram-
CC       positive bacterium S.aureus ATCC 12600 up to a concentration of 100 uM
CC       under low-salt conditions. {ECO:0000269|PubMed:27007913}.
CC   -!- TISSUE SPECIFICITY: Expressed in root nodules but not in seed.
CC       {ECO:0000269|PubMed:27007913}.
CC   -!- DOMAIN: The presence of a 'disulfide through disulfide knot'
CC       structurally defines this protein as a knottin. {ECO:0000305}.
CC   -!- PTM: Contains 3 disulfide bonds. {ECO:0000269|PubMed:27007913}.
CC   -!- PTM: This is a cyclic peptide. {ECO:0000255|PROSITE-ProRule:PRU00395,
CC       ECO:0000269|PubMed:27007913}.
CC   -!- MASS SPECTROMETRY: Mass=3101.297; Method=MALDI;
CC       Evidence={ECO:0000269|PubMed:27007913};
CC   -!- SIMILARITY: Belongs to the cyclotide family.
CC       {ECO:0000303|PubMed:27007913}.
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DR   AlphaFoldDB; C0HJS8; -.
DR   SMR; C0HJS8; -.
DR   GO; GO:0006952; P:defense response; IEA:UniProtKB-KW.
DR   InterPro; IPR005535; Cyclotide.
DR   Pfam; PF03784; Cyclotide; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Disulfide bond; Knottin; Plant defense.
FT   PEPTIDE         1..28
FT                   /note="Cliotide T21"
FT                   /evidence="ECO:0000269|PubMed:27007913"
FT                   /id="PRO_0000436318"
FT   DISULFID        4..17
FT                   /evidence="ECO:0000250|UniProtKB:P58442"
FT   DISULFID        8..19
FT                   /evidence="ECO:0000250|UniProtKB:P58442"
FT   DISULFID        13..25
FT                   /evidence="ECO:0000250|UniProtKB:P58442"
FT   CROSSLNK        1..28
FT                   /note="Cyclopeptide (Asp-Asn)"
FT                   /evidence="ECO:0000269|PubMed:27007913"
SQ   SEQUENCE   28 AA;  3128 MW;  E8028A4CF6E90FD3 CRC64;
     DLQCAETCVH SPCIGPCYCK HGLICYRN