ID   CYC2_KITGR              Reviewed;         311 AA.
AC   Q9AJE3;
DT   05-SEP-2012, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2001, sequence version 1.
DT   25-MAY-2022, entry version 58.
DE   RecName: Full=Terpentetriene synthase;
DE            EC=4.2.3.36;
GN   Name=cyc2;
OS   Kitasatospora griseola (Streptomyces griseolosporeus).
OC   Bacteria; Actinobacteria; Streptomycetales; Streptomycetaceae;
OC   Kitasatospora.
OX   NCBI_TaxID=2064;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], PATHWAY, AND DISRUPTION PHENOTYPE.
RC   STRAIN=MF730-N6;
RX   PubMed=11567009; DOI=10.1128/jb.183.20.6085-6094.2001;
RA   Dairi T., Hamano Y., Kuzuyama T., Itoh N., Furihata K., Seto H.;
RT   "Eubacterial diterpene cyclase genes essential for production of the
RT   isoprenoid antibiotic terpentecin.";
RL   J. Bacteriol. 183:6085-6094(2001).
RN   [2]
RP   FUNCTION, CATALYTIC ACTIVITY, COFACTOR, BIOPHYSICOCHEMICAL PROPERTIES,
RP   PATHWAY, AND SUBUNIT.
RC   STRAIN=MF730-N6;
RX   PubMed=12138123; DOI=10.1074/jbc.m206382200;
RA   Hamano Y., Kuzuyama T., Itoh N., Furihata K., Seto H., Dairi T.;
RT   "Functional analysis of eubacterial diterpene cyclases responsible for
RT   biosynthesis of a diterpene antibiotic, terpentecin.";
RL   J. Biol. Chem. 277:37098-37104(2002).
CC   -!- FUNCTION: Involved in the production of the isoprenoid antibiotic
CC       terpentecin. Converts terpentedienol diphosphate (TDP) into
CC       terpentetriene (TTE). Can also accept geranylgeranyl diphosphate (GGDP)
CC       and farnesyl diphosphate (FDP) as substrates.
CC       {ECO:0000269|PubMed:12138123}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=terpentedienyl diphosphate = diphosphate + terpentetriene;
CC         Xref=Rhea:RHEA:25617, ChEBI:CHEBI:33019, ChEBI:CHEBI:50302,
CC         ChEBI:CHEBI:58821; EC=4.2.3.36;
CC         Evidence={ECO:0000269|PubMed:12138123};
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC         Evidence={ECO:0000269|PubMed:12138123};
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       Kinetic parameters:
CC         KM=7.6 uM for TDP {ECO:0000269|PubMed:12138123};
CC         KM=7.9 uM for GGDP {ECO:0000269|PubMed:12138123};
CC         KM=61.7 uM for FDP {ECO:0000269|PubMed:12138123};
CC         Vmax=114.6 nmol/min/mg enzyme with TDP as substrate
CC         {ECO:0000269|PubMed:12138123};
CC         Vmax=8.8 nmol/min/mg enzyme with GGDP as substrate
CC         {ECO:0000269|PubMed:12138123};
CC         Vmax=15.9 nmol/min/mg enzyme with FDP as substrate
CC         {ECO:0000269|PubMed:12138123};
CC       pH dependence:
CC         Optimum pH is 6.8. {ECO:0000269|PubMed:12138123};
CC       Temperature dependence:
CC         Optimum temperature is 50 degrees Celsius.
CC         {ECO:0000269|PubMed:12138123};
CC   -!- PATHWAY: Antibiotic biosynthesis. {ECO:0000269|PubMed:11567009,
CC       ECO:0000269|PubMed:12138123}.
CC   -!- SUBUNIT: Homodimer. {ECO:0000269|PubMed:12138123}.
CC   -!- DISRUPTION PHENOTYPE: Mutants do not produce terpentecin.
CC       {ECO:0000269|PubMed:11567009}.
CC   -!- SIMILARITY: Belongs to the terpene synthase family. {ECO:0000305}.
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DR   EMBL; AB048795; BAB39207.1; -; Genomic_DNA.
DR   RefSeq; WP_043911627.1; NZ_JXZB01000002.1.
DR   AlphaFoldDB; Q9AJE3; -.
DR   SMR; Q9AJE3; -.
DR   KEGG; ag:BAB39207; -.
DR   BRENDA; 4.2.3.36; 6030.
DR   BRENDA; 4.2.3.B26; 6030.
DR   BRENDA; 4.2.3.B27; 6030.
DR   BRENDA; 4.2.3.B28; 6030.
DR   BRENDA; 4.2.3.B29; 6030.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0052679; F:terpentetriene synthase activity; IDA:UniProtKB.
DR   GO; GO:0017000; P:antibiotic biosynthetic process; IDA:UniProtKB.
DR   Gene3D; 1.10.600.10; -; 1.
DR   InterPro; IPR008949; Isoprenoid_synthase_dom_sf.
DR   SUPFAM; SSF48576; SSF48576; 1.
PE   1: Evidence at protein level;
KW   Antibiotic biosynthesis; Lyase; Magnesium; Metal-binding.
FT   CHAIN           1..311
FT                   /note="Terpentetriene synthase"
FT                   /id="PRO_0000418818"
FT   MOTIF           77..81
FT                   /note="DDXXD motif"
SQ   SEQUENCE   311 AA;  34773 MW;  7D4E9C942B6EB9A2 CRC64;
     MPDAIEFEHE GRRNPNSAEA ESAYSSIIAA LDLQESDYAV ISGHSRIVGA AALVYPDADA
     ETLLAASLWT ACLIVNDDRW DYVQEDGGRL APGEWFDGVT EVVDTWRTAG PRLPDPFFEL
     VRTTMSRLDA ALGAEAADEI GHEIKRAITA MKWEGVWNEY TKKTSLATYL SFRRGYCTMD
     VQVVLDKWIN GGRSFAALRD DPVRRAIDDV VVRFGCLSND YYSWGREKKA VDKSNAVRIL
     MDHAGYDEST ALAHVRDDCV QAITDLDCIE ESIKRSGHLG SHAQELLDYL ACHRPLIYAA
     ATWPTETNRY R