ID   CYC4_AZOVI              Reviewed;         210 AA.
AC   P43302;
DT   01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 2.
DT   03-AUG-2022, entry version 94.
DE   RecName: Full=Cytochrome c4;
DE   Flags: Precursor;
GN   Name=cycA;
OS   Azotobacter vinelandii.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Azotobacter.
OX   NCBI_TaxID=354;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=CA;
RX   PubMed=7619830; DOI=10.1016/0005-2728(95)00043-i;
RA   Ng T.C.N., Laheri A.N., Maier R.J.;
RT   "Cloning, sequencing, and mutagenesis of the cytochrome c4 gene from
RT   Azotobacter vinelandii: characterization of the mutant strain and a
RT   proposed new branch in the respiratory chain.";
RL   Biochim. Biophys. Acta 1230:119-129(1995).
RN   [2]
RP   PROTEIN SEQUENCE OF 21-210.
RC   STRAIN=ATCC 13705 / OP1 / DSM 366 / NCIMB 11614 / LMG 3878 / UW;
RX   PubMed=6089759; DOI=10.1042/bj2220217;
RA   Ambler R.P., Daniel M., Melis K., Stout C.D.;
RT   "The amino acid sequence of the dihaem cytochrome c4 from the bacterium
RT   Azotobacter vinelandii.";
RL   Biochem. J. 222:217-227(1984).
CC   -!- FUNCTION: Diheme, high potential cytochrome c believed to be an
CC       intermediate electron donor to terminal oxidation systems.
CC   -!- SUBCELLULAR LOCATION: Periplasm.
CC   -!- PTM: Binds 2 heme c groups covalently per subunit.
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DR   EMBL; L37290; AAA87314.1; -; Genomic_DNA.
DR   PIR; I39740; I39740.
DR   RefSeq; WP_012698889.1; NZ_FPKM01000002.1.
DR   AlphaFoldDB; P43302; -.
DR   SMR; P43302; -.
DR   OMA; AGCHSPN; -.
DR   GO; GO:0042597; C:periplasmic space; IEA:UniProtKB-SubCell.
DR   GO; GO:0009055; F:electron transfer activity; IEA:InterPro.
DR   GO; GO:0020037; F:heme binding; IEA:InterPro.
DR   GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR   Gene3D; 1.10.760.10; -; 2.
DR   InterPro; IPR009056; Cyt_c-like_dom.
DR   InterPro; IPR036909; Cyt_c-like_dom_sf.
DR   InterPro; IPR024167; Cytochrome_c4-like.
DR   Pfam; PF00034; Cytochrom_C; 2.
DR   PIRSF; PIRSF000005; Cytochrome_c4; 1.
DR   SUPFAM; SSF46626; SSF46626; 2.
DR   PROSITE; PS51007; CYTC; 2.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Electron transport; Heme; Iron; Metal-binding;
KW   Periplasm; Signal; Transport.
FT   SIGNAL          1..20
FT                   /evidence="ECO:0000269|PubMed:6089759"
FT   CHAIN           21..210
FT                   /note="Cytochrome c4"
FT                   /id="PRO_0000006507"
FT   BINDING         34
FT                   /ligand="heme c"
FT                   /ligand_id="ChEBI:CHEBI:61717"
FT                   /ligand_label="1"
FT                   /note="covalent"
FT   BINDING         37
FT                   /ligand="heme c"
FT                   /ligand_id="ChEBI:CHEBI:61717"
FT                   /ligand_label="1"
FT                   /note="covalent"
FT   BINDING         38
FT                   /ligand="heme c"
FT                   /ligand_id="ChEBI:CHEBI:61717"
FT                   /ligand_label="1"
FT                   /ligand_part="Fe"
FT                   /ligand_part_id="ChEBI:CHEBI:18248"
FT                   /note="axial binding residue"
FT   BINDING         86
FT                   /ligand="heme c"
FT                   /ligand_id="ChEBI:CHEBI:61717"
FT                   /ligand_label="1"
FT                   /ligand_part="Fe"
FT                   /ligand_part_id="ChEBI:CHEBI:18248"
FT                   /note="axial binding residue"
FT   BINDING         139
FT                   /ligand="heme c"
FT                   /ligand_id="ChEBI:CHEBI:61717"
FT                   /ligand_label="2"
FT                   /note="covalent"
FT   BINDING         142
FT                   /ligand="heme c"
FT                   /ligand_id="ChEBI:CHEBI:61717"
FT                   /ligand_label="2"
FT                   /note="covalent"
FT   BINDING         143
FT                   /ligand="heme c"
FT                   /ligand_id="ChEBI:CHEBI:61717"
FT                   /ligand_label="2"
FT                   /ligand_part="Fe"
FT                   /ligand_part_id="ChEBI:CHEBI:18248"
FT                   /note="axial binding residue"
FT   BINDING         187
FT                   /ligand="heme c"
FT                   /ligand_id="ChEBI:CHEBI:61717"
FT                   /ligand_label="2"
FT                   /ligand_part="Fe"
FT                   /ligand_part_id="ChEBI:CHEBI:18248"
FT                   /note="axial binding residue"
SQ   SEQUENCE   210 AA;  21687 MW;  4B09C4AA90D1FC74 CRC64;
     MNKALVTLLL TLGITGLAHA AGDAAAGQGK AAVCGACHGP DGNSAAPNFP KLAGQGERYL
     LKQMQDIKAG TKPGAPEGSG RKVLEMTGML DNFSDQDLAD LAAYFTSQKP TVGAADPQLV
     EAGETLYRGG KLADGMPACT GCHSPNGEGN TPAAYPRLSG QHAQYVAKQL TDFREGARTN
     DGDNMIMRSI AAKLSNKDIA AISSYIQGLH