CYC8_DICDI
ID CYC8_DICDI Reviewed; 1390 AA.
AC O77033; Q55DQ3;
DT 08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1998, sequence version 1.
DT 25-MAY-2022, entry version 122.
DE RecName: Full=General transcriptional corepressor trfA;
GN Name=trfA; ORFNames=DDB_G0269194;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], AND INDUCTION.
RC STRAIN=AX2;
RX PubMed=9733762; DOI=10.1074/jbc.273.38.24654;
RA Saito J., Kon T., Nagasaki A., Adachi H., Sutoh K.;
RT "Dictyostelium TRFA homologous to yeast Ssn6 is required for normal growth
RT and early development.";
RL J. Biol. Chem. 273:24654-24659(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
CC -!- FUNCTION: Acts as component of the trfA-tupA corepressor complex which
CC is involved in the repression of many genes in a wide variety of
CC physiological processes. May also be involved in the derepression of at
CC least some target genes. The complex is recruited to target genes by
CC interaction with DNA-bound transcriptional repressors. The complex
CC recruits histone deacetylases to produce a repressive chromatin
CC structure, interacts with hypoacetylated N-terminal tails of histones
CC H3 and H4 that have been programmed for repression by the action of
CC histone deacetylases and interferes directly with the transcriptional
CC machinery by associating with the RNA polymerase II mediator complex
CC (By similarity). Required for normal growth and for aggregation in
CC early development. Required for a proper chemotactic response to cAMP.
CC {ECO:0000250}.
CC -!- SUBUNIT: Associates with tupA to form the trfA-tupA corepressor
CC complex. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC -!- INDUCTION: Expressed at the vegetative stage and at an early
CC developmental stage, although at a lower level.
CC {ECO:0000269|PubMed:9733762}.
CC -!- SIMILARITY: Belongs to the CYC8/SSN6 family. {ECO:0000305}.
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DR EMBL; AB009080; BAA33143.1; -; Genomic_DNA.
DR EMBL; AAFI02000005; EAL71947.1; -; Genomic_DNA.
DR PIR; T14004; T14004.
DR RefSeq; XP_646078.1; XM_640986.1.
DR AlphaFoldDB; O77033; -.
DR SMR; O77033; -.
DR STRING; 44689.DDB0191270; -.
DR PaxDb; O77033; -.
DR PRIDE; O77033; -.
DR EnsemblProtists; EAL71947; EAL71947; DDB_G0269194.
DR GeneID; 8617028; -.
DR KEGG; ddi:DDB_G0269194; -.
DR dictyBase; DDB_G0269194; trfA.
DR eggNOG; KOG1124; Eukaryota.
DR HOGENOM; CLU_255023_0_0_1; -.
DR InParanoid; O77033; -.
DR OMA; TSDIWFQ; -.
DR Reactome; R-DDI-2559580; Oxidative Stress Induced Senescence.
DR Reactome; R-DDI-3214842; HDMs demethylate histones.
DR PRO; PR:O77033; -.
DR Proteomes; UP000002195; Chromosome 1.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0031490; F:chromatin DNA binding; IBA:GO_Central.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0010468; P:regulation of gene expression; IBA:GO_Central.
DR GO; GO:0030587; P:sorocarp development; IMP:dictyBase.
DR Gene3D; 1.25.40.10; -; 3.
DR InterPro; IPR011990; TPR-like_helical_dom_sf.
DR InterPro; IPR001440; TPR_1.
DR InterPro; IPR019734; TPR_repeat.
DR Pfam; PF00515; TPR_1; 1.
DR Pfam; PF13181; TPR_8; 5.
DR SMART; SM00028; TPR; 9.
DR SUPFAM; SSF48452; SSF48452; 2.
DR PROSITE; PS50005; TPR; 9.
DR PROSITE; PS50293; TPR_REGION; 1.
PE 2: Evidence at transcript level;
KW Nucleus; Reference proteome; Repeat; Repressor; TPR repeat; Transcription;
KW Transcription regulation.
FT CHAIN 1..1390
FT /note="General transcriptional corepressor trfA"
FT /id="PRO_0000327392"
FT REPEAT 171..204
FT /note="TPR 1"
FT REPEAT 206..238
FT /note="TPR 2"
FT REPEAT 239..272
FT /note="TPR 3"
FT REPEAT 275..308
FT /note="TPR 4"
FT REPEAT 312..345
FT /note="TPR 5"
FT REPEAT 349..382
FT /note="TPR 6"
FT REPEAT 384..419
FT /note="TPR 7"
FT REPEAT 420..453
FT /note="TPR 8"
FT REPEAT 454..487
FT /note="TPR 9"
FT REPEAT 489..521
FT /note="TPR 10"
FT REGION 53..143
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 539..596
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 632..938
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 958..1390
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 577..596
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 643..742
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 743..802
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 803..857
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 872..938
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 958..1104
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1114..1131
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1132..1146
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1147..1169
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1190..1204
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1218..1242
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1243..1270
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1271..1294
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1309..1337
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1338..1390
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1390 AA; 160421 MW; BAAB9926656002DE CRC64;
MNVQQTQQQQ AMAAQQQSMV AQQQQQIAQQ QSAIAQQQIA QQQQIAQQQQ IAQQQQQHQQ
HQQHQQQHQQ QQQHQQQHQQ QQHIQQQQQP QQQQPQQQQS QQQQQQHQQQ QQPQQQQPPQ
QQQQQPPQQQ QQPQQQQQQQ QQQQQQPQQQ QQQQQQDQLS GVTGKIRDLN ESIWIHLGGY
AESIGEQDKA LASYENALRH NPFSIKALTQ IASLFRIKEQ YSKAAEYFQR IVTIESKNGE
VWGALGHCYL MMDDLQKAYT AYQQALYHLP NPKDPNLWYG IGILYDRYGS YDHAEEAFTA
VLKMDNKFEK STEIYFRLGV LYKHQGKYDQ SLEYFQHLVK NPPLPLTTSD IWFQIGHVYE
LQKEYHKSKD AYEKVLKDNA THSKVLQQLG WLYHHNPLFT NQEYAINYLM RSIDSDSSDA
QTWYLLGRCY MTQQKYKKAY DAYQQAVYRD GRNPTFWCSI GVLYYQINQY RDALDAYTRA
IRLNPFLSEV WYDLGTLYES CHQHTDSLDA YQRAAELDPH NKHIQSRLAT LRAQVSGKPI
GKDGYDLQNG EHGEHGGKST PMIIEPNSPG TGAMESLGKG GQNNRNGNNN GNNSFVPELA
EINSHLPEDM RNNSSLPSSN ELNSSLIDRK TERGRGEDMH NSHHSQYSNS MSMNNMNNNN
NNMNMNNNMN NNMNNMNNNN NNMNSMNNMN NNNNNINNNM SNNNNNNHNN HQMNQYNNNN
NNSNINNNNN NHHNMNDNVN SKNNDVLDRR YKGILEREKT SPNGDGRDNR DNIRDNRDNR
DSRDGRDNRD GRDSRDRIQE YTREYNNNNN NNNSISSINN NNNNNNNNYN NNNNNNNNNN
NNNNNNGLRA TSPLPSHNDR RSYERDNKER INNNNNNNNN MNNNMNNNMN NMNNNNNNSN
ISRFEHQNNR TSSPFENNSN NNNNNNNNNN NNINYNNIGQ RALSPQSSQH KDRREIILDE
ESDINERSKT RSPSIVKEAE EKRETVIVDK ERSPTPIITE KPDEKQVEKV TDKESSLVEK
VDKENEKESP SSSSSSKEIE KETEKEKEKE KEKEKEVEKE VEKEIENDKE KEKEKEVEKD
VEENKSVEKS SEKPVEKEST TTTTNDEDEE GELSEPTTTT KKDDSSKLPT DEKKLSSVSP
TTTAVEQSRD ETKELEMDTK EDSEKEKKSS TTTTAAASES VKPIDEEKKS PTTTTTTTTN
TTTVEPTHKD KESSKNDDTT TTTTTTTTKS AKSPNSSPTR SDEVVEPHQD ASQEEINKRK
LEDDITSTPS KRLKPDSTPS SATTASTPSE QPESPLKKEN PVGETLSPEI KDSKSSSSSS
SSSSSSTNTG SSSTNSSAKN ERDRDRERER ERERDRERER ERERERERER EKNKQPTRAP
IKPSSRKLER
